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The map label for this gene is miaA

Identifier: 116872725

GI number: 116872725

Start: 1301292

End: 1302209

Strand: Direct

Name: miaA

Synonym: lwe1309

Alternate gene names: 116872725

Gene position: 1301292-1302209 (Clockwise)

Preceding gene: 116872724

Following gene: 116872726

Centisome position: 46.24

GC content: 37.25

Gene sequence:

>918_bases
TTGAGCAAGATTCCTGTCATCGTCATCGTTGGCCCAACTGCTGTCGGCAAAACAAGTCTTAGTATTGAATTAGCGAAAAA
GTTGGATGGAGAAATCATTAGTGGCGACTCTATGCAAGTATATCGTGGGTTAGATATCGGTACAGCAAAAATCACATCAG
AAGAAATGTGCGGTATAAAACATCACCTTATTGATGTCACAGATGCGTCTGTGCCATTCACTGCAGCGAAATTCCAATCA
GAAACAATGGCTTTGATTGAATCCATACATAGCCGCGGAAAACTTCCTATTATAGTTGGCGGGACGGGTCTTTACATTCA
GTCTGTTTTTTATGATTATGATTTTGGGAATTCAAGCGAGGATAAGGCGTTCCGAGCAAAATTAGATTCTTTAGACAAAG
TAACTTTATGGAAAATGCTAGAACAACAAGACCCTGAAAGCGCAAAACTTATTCATGAAAACAATAAAAGGCGCGTTATT
AGAGCGTTGGAAGTGATACACCTAACCGGAAAACCTTTTTCCGAATATCAAGTTCATCATAAATTAAATGATATGTATAA
GCCTCTTTTTTTAGGACTTGATTTAGATAGGGCATTACTTTATGAAAGAATCAATCAGCGCGTAGATATAATGTTTGAAC
AAGGACTAGTTTCAGAAGCAAAAAAACTATACGATGAGAACCTAGCTGATGTACCAGCCATTCGTGGTATAGGTTACAAA
GAATTATTTACCTATTTTGAGGGCAATAGTACACTGGAAGAAGCGAAAGAATTAATTCAAAAAAACTCGCGTCATTTCGC
GAAAAGACAGTTAACTTGGTTTAGGAATAGGATGGAAATTAACTGGATTCAAGCTGGTACCAGTTCAACTGAAACCGAAG
CAATGAATAAAGTGAAGACCTTTTTAGCATCTAAATAA

Upstream 100 bases:

>100_bases
TTTTGAGATTTGCGCTTTTTTTATGAAAAAAAGCATTTTCGCTATTTTCAAGAACCCATTTTCATGGTACATTGATTACA
GACGAAAAGGAGGTTTTTTT

Downstream 100 bases:

>100_bases
TACTTTTCTGTTTGGTAGTAAGAAATAAAGGGTATTTGAAATTAGCCAACCAATTGCACTATGGAGTATATTGGGGTTTG
GGAAATTTTTTAGTATAAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITSEEMCGIKHHLIDVTDASVPFTAAKFQS
ETMALIESIHSRGKLPIIVGGTGLYIQSVFYDYDFGNSSEDKAFRAKLDSLDKVTLWKMLEQQDPESAKLIHENNKRRVI
RALEVIHLTGKPFSEYQVHHKLNDMYKPLFLGLDLDRALLYERINQRVDIMFEQGLVSEAKKLYDENLADVPAIRGIGYK
ELFTYFEGNSTLEEAKELIQKNSRHFAKRQLTWFRNRMEINWIQAGTSSTETEAMNKVKTFLASK

Sequences:

>Translated_305_residues
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITSEEMCGIKHHLIDVTDASVPFTAAKFQS
ETMALIESIHSRGKLPIIVGGTGLYIQSVFYDYDFGNSSEDKAFRAKLDSLDKVTLWKMLEQQDPESAKLIHENNKRRVI
RALEVIHLTGKPFSEYQVHHKLNDMYKPLFLGLDLDRALLYERINQRVDIMFEQGLVSEAKKLYDENLADVPAIRGIGYK
ELFTYFEGNSTLEEAKELIQKNSRHFAKRQLTWFRNRMEINWIQAGTSSTETEAMNKVKTFLASK
>Mature_304_residues
SKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITSEEMCGIKHHLIDVTDASVPFTAAKFQSE
TMALIESIHSRGKLPIIVGGTGLYIQSVFYDYDFGNSSEDKAFRAKLDSLDKVTLWKMLEQQDPESAKLIHENNKRRVIR
ALEVIHLTGKPFSEYQVHHKLNDMYKPLFLGLDLDRALLYERINQRVDIMFEQGLVSEAKKLYDENLADVPAIRGIGYKE
LFTYFEGNSTLEEAKELIQKNSRHFAKRQLTWFRNRMEINWIQAGTSSTETEAMNKVKTFLASK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=34.2948717948718, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=35.3741496598639, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=31.8032786885246, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=38.8316151202749, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=32.8358208955224, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LISW6 (A0AI95)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_849506.1
- ProteinModelPortal:   A0AI95
- SMR:   A0AI95
- STRING:   A0AI95
- GeneID:   4465330
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe1309
- NMPDR:   fig|386043.6.peg.1268
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A0AI95
- ProtClustDB:   PRK00091
- BioCyc:   LWEL386043:LWE1309-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34568; Mature: 34437

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITSEEMCGIK
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCEECCHHHHHCHH
HHLIDVTDASVPFTAAKFQSETMALIESIHSRGKLPIIVGGTGLYIQSVFYDYDFGNSSE
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHEECCCCCCCC
DKAFRAKLDSLDKVTLWKMLEQQDPESAKLIHENNKRRVIRALEVIHLTGKPFSEYQVHH
CHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHH
KLNDMYKPLFLGLDLDRALLYERINQRVDIMFEQGLVSEAKKLYDENLADVPAIRGIGYK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHH
ELFTYFEGNSTLEEAKELIQKNSRHFAKRQLTWFRNRMEINWIQAGTSSTETEAMNKVKT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
FLASK
HHCCC
>Mature Secondary Structure 
SKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITSEEMCGIK
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCEECCHHHHHCHH
HHLIDVTDASVPFTAAKFQSETMALIESIHSRGKLPIIVGGTGLYIQSVFYDYDFGNSSE
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHEECCCCCCCC
DKAFRAKLDSLDKVTLWKMLEQQDPESAKLIHENNKRRVIRALEVIHLTGKPFSEYQVHH
CHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHH
KLNDMYKPLFLGLDLDRALLYERINQRVDIMFEQGLVSEAKKLYDENLADVPAIRGIGYK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHH
ELFTYFEGNSTLEEAKELIQKNSRHFAKRQLTWFRNRMEINWIQAGTSSTETEAMNKVKT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
FLASK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA