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The map label for this gene is cbiP [H]

Identifier: 116872581

GI number: 116872581

Start: 1172132

End: 1173667

Strand: Direct

Name: cbiP [H]

Synonym: lwe1165

Alternate gene names: 116872581

Gene position: 1172132-1173667 (Clockwise)

Preceding gene: 116872580

Following gene: 116872582

Centisome position: 41.65

GC content: 37.83

Gene sequence:

>1536_bases
GTGGTAGAACAAATAATGATTCAAGGTACCGCCTCTGATGCAGGTAAAAGTATATTAGTTGCAGGATTATGTCGCCTATT
CAAAAATAAGGGCAAGCGGGTCGTTCCATTTAAATCGCAAAATATGTCGCTTAATTCTTTTATTACTGCAACTGGAGATG
AAATGGGTCGTGCACAAGTTTTTCAAGCAGAAGCAGCTGGCGTTTTTCCAGATGTTCGAATGAATCCTGTTTTACTCAAA
CCGACAAATGACCGCCAGTCACAAGTGGTTTTTATGGGTTCGATTTTAGATAATATGGATGCAGTTACCTATCATGACTT
CAAGCAAACACTTATTTCAAAAATCCAGGCAGTTTATCAGAGTTTAGCGGATGAAAATGATATTATTGTACTAGAAGGCG
CTGGAAGCCCAGCCGAAATCAATTTAAACGATCGTGATATCGTCAATATGGGCATGGCAAAAATGGTCGATGCGCCAGTT
GTTTTAGTAGCTGATATTGATAAAGGTGGTGTGTTTGCTTCAATTTATGGCACAATAATGCTCTTAAATGAAGAAGAGAG
CGCCAGAATTAAGGGCGTTATCATTAATAAATTCCGCGGGGATGTAGCTCTTTTACAACCCGGAATTGATATGATAGAAG
AACTCACTAAAGTCCCAGTTATCGGTGTGATACCATACGCCAATCTAAAGCTAGAAGAAGAAGATAGTGTCTCACTTAGC
GGAAAAAATTACGTGCCAAATAGTTCGGCATTACTAGATATCGCGATTATTTGTTTACCGCGGATTTCTAATTTCACTGA
TTTTCATGTTCTGGAAATTCAACCTGACATTAGTTTACGCTATATACGAAATCTAGCGGAGTTCGGAAATCCTGATTTAG
TCATTATCCCAGGTAGTAAAAATACGTTAGAAGATATGGCTTTTCTGGAAAAATCAGGCTTAAAAAAAGCCATTCAACAC
TATGCTGAAAAAGCTGGAAAAGTAATTGGTATCTGCGGTGGATATCAAATGCTTGGTAAAAAAATGCTTGATCCAAATCA
AGTGGAAAGTGAACAAATAGAAATTTCAGGACTTGGCTTACTTGATACCGAAACCATTTTCCTAAATCAAAAACGCACGA
CGCAAATTACTGGCGTGACACTTTCTGGTGAACCAGTGGAAGGATACGAGATCCATATGGGGCAGACAAAGCGAGGCGAG
AGCACTAGCCCATTTTGCGAAATTAAAGCAGTAAATGGCAACCAAGAAACCCACCAAGATGGCGCCGTTTCCACAAACAA
AAACATCATTGGCACTTATATTCACGGCATTTTTGATAATGATATTTTCTTAGGAAATCTATTCAACGAACTACTAACAC
TCAAAAATAAAACCGTTTATCCACATGAAATCATCCAATTAAAAGAACATAAAGAACAAGAATATGACAAACTAGCGGCC
CTTTTAGAAGAAAATATTCAAATGGACCAACTAGAAAAAATTATGAAAGGAGAAAAAATATGCGTATCTACACAAAAACC
GGTGATAAAGGAATGA

Upstream 100 bases:

>100_bases
TAGTGCAGCCTTGGCTCATCAAATTACATCAACAAGCTGGTTATCCGCTTTTTAAAAAAGAAGCCGATTTTTTCGCGCAC
ACGAGGAAGGTGACTATGTA

Downstream 100 bases:

>100_bases
CAAGAATTATCGGGGGCAGTAAAGTAAGTAAAGACAATGTTCGAATCGATGCATATGGCACATTAGATGAGCTGAACTCG
CTGATTGGTTTCACAATTAC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEQIMIQGTASDAGKSILVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVVFMGSILDNMDAVTYHDFKQTLISKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEESARIKGVIINKFRGDVALLQPGIDMIEELTKVPVIGVIPYANLKLEEEDSVSLS
GKNYVPNSSALLDIAIICLPRISNFTDFHVLEIQPDISLRYIRNLAEFGNPDLVIIPGSKNTLEDMAFLEKSGLKKAIQH
YAEKAGKVIGICGGYQMLGKKMLDPNQVESEQIEISGLGLLDTETIFLNQKRTTQITGVTLSGEPVEGYEIHMGQTKRGE
STSPFCEIKAVNGNQETHQDGAVSTNKNIIGTYIHGIFDNDIFLGNLFNELLTLKNKTVYPHEIIQLKEHKEQEYDKLAA
LLEENIQMDQLEKIMKGEKICVSTQKPVIKE

Sequences:

>Translated_511_residues
MVEQIMIQGTASDAGKSILVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVVFMGSILDNMDAVTYHDFKQTLISKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEESARIKGVIINKFRGDVALLQPGIDMIEELTKVPVIGVIPYANLKLEEEDSVSLS
GKNYVPNSSALLDIAIICLPRISNFTDFHVLEIQPDISLRYIRNLAEFGNPDLVIIPGSKNTLEDMAFLEKSGLKKAIQH
YAEKAGKVIGICGGYQMLGKKMLDPNQVESEQIEISGLGLLDTETIFLNQKRTTQITGVTLSGEPVEGYEIHMGQTKRGE
STSPFCEIKAVNGNQETHQDGAVSTNKNIIGTYIHGIFDNDIFLGNLFNELLTLKNKTVYPHEIIQLKEHKEQEYDKLAA
LLEENIQMDQLEKIMKGEKICVSTQKPVIKE
>Mature_511_residues
MVEQIMIQGTASDAGKSILVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVVFMGSILDNMDAVTYHDFKQTLISKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEESARIKGVIINKFRGDVALLQPGIDMIEELTKVPVIGVIPYANLKLEEEDSVSLS
GKNYVPNSSALLDIAIICLPRISNFTDFHVLEIQPDISLRYIRNLAEFGNPDLVIIPGSKNTLEDMAFLEKSGLKKAIQH
YAEKAGKVIGICGGYQMLGKKMLDPNQVESEQIEISGLGLLDTETIFLNQKRTTQITGVTLSGEPVEGYEIHMGQTKRGE
STSPFCEIKAVNGNQETHQDGAVSTNKNIIGTYIHGIFDNDIFLGNLFNELLTLKNKTVYPHEIIQLKEHKEQEYDKLAA
LLEENIQMDQLEKIMKGEKICVSTQKPVIKE

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 56341; Mature: 56341

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQIMIQGTASDAGKSILVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQV
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCEE
FQAEAAGVFPDVRMNPVLLKPTNDRQSQVVFMGSILDNMDAVTYHDFKQTLISKIQAVYQ
EEHHCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
SLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPVVLVADIDKGGVFASIYGTIM
HHCCCCCEEEEECCCCCCEEECCCCHHHHHCHHHHCCCCEEEEEECCCCCEEEEEEEEEE
LLNEEESARIKGVIINKFRGDVALLQPGIDMIEELTKVPVIGVIPYANLKLEEEDSVSLS
EEECCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHCCEEEEECCCCEEECCCCCEEEC
GKNYVPNSSALLDIAIICLPRISNFTDFHVLEIQPDISLRYIRNLAEFGNPDLVIIPGSK
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCC
NTLEDMAFLEKSGLKKAIQHYAEKAGKVIGICGGYQMLGKKMLDPNQVESEQIEISGLGL
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCE
LDTETIFLNQKRTTQITGVTLSGEPVEGYEIHMGQTKRGESTSPFCEIKAVNGNQETHQD
EECEEEEEECCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GAVSTNKNIIGTYIHGIFDNDIFLGNLFNELLTLKNKTVYPHEIIQLKEHKEQEYDKLAA
CCCCCCCCEEEEEEEEEECCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLEENIQMDQLEKIMKGEKICVSTQKPVIKE
HHHHCCCHHHHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MVEQIMIQGTASDAGKSILVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQV
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCEE
FQAEAAGVFPDVRMNPVLLKPTNDRQSQVVFMGSILDNMDAVTYHDFKQTLISKIQAVYQ
EEHHCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
SLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPVVLVADIDKGGVFASIYGTIM
HHCCCCCEEEEECCCCCCEEECCCCHHHHHCHHHHCCCCEEEEEECCCCCEEEEEEEEEE
LLNEEESARIKGVIINKFRGDVALLQPGIDMIEELTKVPVIGVIPYANLKLEEEDSVSLS
EEECCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHCCEEEEECCCCEEECCCCCEEEC
GKNYVPNSSALLDIAIICLPRISNFTDFHVLEIQPDISLRYIRNLAEFGNPDLVIIPGSK
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCC
NTLEDMAFLEKSGLKKAIQHYAEKAGKVIGICGGYQMLGKKMLDPNQVESEQIEISGLGL
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCE
LDTETIFLNQKRTTQITGVTLSGEPVEGYEIHMGQTKRGESTSPFCEIKAVNGNQETHQD
EECEEEEEECCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GAVSTNKNIIGTYIHGIFDNDIFLGNLFNELLTLKNKTVYPHEIIQLKEHKEQEYDKLAA
CCCCCCCCEEEEEEEEEECCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLEENIQMDQLEKIMKGEKICVSTQKPVIKE
HHHHCCCHHHHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]