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The map label for this gene is ackA2 [H]

Identifier: 116872542

GI number: 116872542

Start: 1137942

End: 1139135

Strand: Direct

Name: ackA2 [H]

Synonym: lwe1126

Alternate gene names: 116872542

Gene position: 1137942-1139135 (Clockwise)

Preceding gene: 116872541

Following gene: 116872543

Centisome position: 40.44

GC content: 37.6

Gene sequence:

>1194_bases
ATGCACAAAATTATGGCGATAAACGCAGGGAGTTCTTCACTGAAATTCCAAATTTTTACGATGCCAGGAGAAGAAGTTTT
AGTTAAGGGCTTAATTGAAAGAATCGGATTACCAGATGCCATTTTCAACATGTCCTTTCAAAATGAAAAAATCAAAGAAA
CGCGATCAATTAATAACCACGGAGAAGCTGTCGAAATTTTACTAGAGCAATTGAAGGCTCATCAAGTAATTAATGATTTA
AATGAAATTACTGGTGTCGGGCATCGCGTGGCTCATGGTGGGGAAGCTTTTGTTACATCGTGTATTGTGACAGATGAAGT
AGTGAAAGGCATTGAAGATGTAACAAGTCTTGCTCCGTTACACAATCCAGCTAATATTATCGGTATTAAAACTTTCCGTA
AACTGTTGCCAAATGCTGTTTCTGTGGCTGTATTTGATACGGCTTATCACCAAACCATCCCTGAAGAAAACTTTTTATAT
GCGCTTCCTTATGAACTTTATGAAAAACATCATATCAGAAAATACGGTTTTCATGGAACAAGTCATAAATATGTTGCTGG
AAAAGCAGCTGAAGTTCTCAAAAAACCTTTAGAAGAATTAAAAATTATTTCTTGTCATTTAGGTAATGGTGCAAGTGTTT
GTGCGATTGAAGCTGGTAAATCAGTGAATACATCCATGGGCTTTACGCCAAACGCTGGCTTGATGATGGGAACACGTTCT
GGTACAATTGACGCGACAATCATCCCGTATTTGGTCGATGAACTTGGCTATAGCTTAGACGAAGTAATGCATATGATGTC
TAGTCAATCTGGCGTTCTTGGGGTATCAGGTAGTTCAAGTGATTTTAGAGATATTGAGATTGCTGCGGAAGAAGGAGATT
CCCGTGCATTACTTACGCTTCGAATGTTTACTGGCCAAATCTGTAATTATATTGGGGCTTATGCCGCGGCAATGAACGGT
TGCGACGCGTTATTATTTACAGCCGGAGTTGGTGAAAATTCTTCGTTAATCCGTAAAATGGTTACAGAACAGTTAAGTTA
CCTTGGTGTAACGTGTAATGTGACAAAAAATAATGCGGGTGATATGATAATTAGCGATGACAATGAAGCAGTCAAAGTAT
GTATTATTCCAACAAATGAAGAACTAATGATTGCTCGTGATGTAGAAAAATATACAAAACAAACAATAAGTTAA

Upstream 100 bases:

>100_bases
CTTGCCATCGGACTGTATAACATTTTATTATAAAAAATAAGATGGAGTAAACAATGATTAGATTGTTTGCTCCTTTAAAA
ATGAGAAAGGACTGAGCTAT

Downstream 100 bases:

>100_bases
GGAGCGATTTGATGTGAAAATAACGACGGCAATGCACGGTGGCAACTATAATGAACTAGCAAAACAACATGGACTAACCA
AAGAAATGGTACTTGATTTT

Product: acetate kinase 2

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKETRSINNHGEAVEILLEQLKAHQVINDL
NEITGVGHRVAHGGEAFVTSCIVTDEVVKGIEDVTSLAPLHNPANIIGIKTFRKLLPNAVSVAVFDTAYHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLKKPLEELKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSQSGVLGVSGSSSDFRDIEIAAEEGDSRALLTLRMFTGQICNYIGAYAAAMNG
CDALLFTAGVGENSSLIRKMVTEQLSYLGVTCNVTKNNAGDMIISDDNEAVKVCIIPTNEELMIARDVEKYTKQTIS

Sequences:

>Translated_397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKETRSINNHGEAVEILLEQLKAHQVINDL
NEITGVGHRVAHGGEAFVTSCIVTDEVVKGIEDVTSLAPLHNPANIIGIKTFRKLLPNAVSVAVFDTAYHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLKKPLEELKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSQSGVLGVSGSSSDFRDIEIAAEEGDSRALLTLRMFTGQICNYIGAYAAAMNG
CDALLFTAGVGENSSLIRKMVTEQLSYLGVTCNVTKNNAGDMIISDDNEAVKVCIIPTNEELMIARDVEKYTKQTIS
>Mature_397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKETRSINNHGEAVEILLEQLKAHQVINDL
NEITGVGHRVAHGGEAFVTSCIVTDEVVKGIEDVTSLAPLHNPANIIGIKTFRKLLPNAVSVAVFDTAYHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLKKPLEELKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSQSGVLGVSGSSSDFRDIEIAAEEGDSRALLTLRMFTGQICNYIGAYAAAMNG
CDALLFTAGVGENSSLIRKMVTEQLSYLGVTCNVTKNNAGDMIISDDNEAVKVCIIPTNEELMIARDVEKYTKQTIS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=43.859649122807, Blast_Score=317, Evalue=6e-88,
Organism=Escherichia coli, GI145693184, Length=388, Percent_Identity=42.0103092783505, Blast_Score=317, Evalue=1e-87,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43139; Mature: 43139

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKETRSINNH
CCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCCC
GEAVEILLEQLKAHQVINDLNEITGVGHRVAHGGEAFVTSCIVTDEVVKGIEDVTSLAPL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPANIIGIKTFRKLLPNAVSVAVFDTAYHQTIPEENFLYALPYELYEKHHIRKYGFHGT
CCCCCEEHHHHHHHHCCCCEEEEEEEHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCC
SHKYVAGKAAEVLKKPLEELKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
CCCEECCHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GTIDATIIPYLVDELGYSLDEVMHMMSSQSGVLGVSGSSSDFRDIEIAAEEGDSRALLTL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEH
RMFTGQICNYIGAYAAAMNGCDALLFTAGVGENSSLIRKMVTEQLSYLGVTCNVTKNNAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCC
DMIISDDNEAVKVCIIPTNEELMIARDVEKYTKQTIS
CEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKETRSINNH
CCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCCC
GEAVEILLEQLKAHQVINDLNEITGVGHRVAHGGEAFVTSCIVTDEVVKGIEDVTSLAPL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPANIIGIKTFRKLLPNAVSVAVFDTAYHQTIPEENFLYALPYELYEKHHIRKYGFHGT
CCCCCEEHHHHHHHHCCCCEEEEEEEHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCC
SHKYVAGKAAEVLKKPLEELKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
CCCEECCHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GTIDATIIPYLVDELGYSLDEVMHMMSSQSGVLGVSGSSSDFRDIEIAAEEGDSRALLTL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEH
RMFTGQICNYIGAYAAAMNGCDALLFTAGVGENSSLIRKMVTEQLSYLGVTCNVTKNNAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCC
DMIISDDNEAVKVCIIPTNEELMIARDVEKYTKQTIS
CEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA