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The map label for this gene is nicK [H]

Identifier: 116872194

GI number: 116872194

Start: 803827

End: 805128

Strand: Direct

Name: nicK [H]

Synonym: lwe0774

Alternate gene names: 116872194

Gene position: 803827-805128 (Clockwise)

Preceding gene: 116872193

Following gene: 116872195

Centisome position: 28.56

GC content: 33.33

Gene sequence:

>1302_bases
ATGAAAGGAGAACAAATAAAAAAATTACGCCAACGAATAGGGATGAACCAAACGACTTTGGCCGAAAAGCTTGAAATATC
TAAAGGATATTTATCAAAAATAGAGAACAATCAAAAGAAGCTTAATTCGTCTATTATTAATAAACTGCAATCAATTTTCC
ATTTGACTGGTGGAATTTTGCCTATGGAAGCTAAAATAGATTTTTTACGAATTCGTTTAAAAATTGATTGCCCTACTCAA
GTGATTACAAATATATTAAAAATGGACCCCAATGTTTTTGGATACAAATCTTATGGGTTCAACCATTATACAGAAACATA
TTTTTTTAGTGAAATATTTATTTTCTCAAACCCCAATGATATTAATATGGGTGTCATGATTGAGTTAAGAGGTCAAGGGT
GCAGAGAGTATGAACTGATTTTGGAAGAGAGAAAAGAAAACTGGAATGATTTCTTTTGGAGACTGTATCAAGATGACCTT
TTTGGTAAAGGGGTTTTGATAGATACCAAAATTACTCGGATTGATATTGCATTAGATGAAATGATCTCGTGTTTATATAA
AAATTATGATTTACATGAGTTAAAGCGAAAATGTGAACAAGGATTAGTGGATACTACATTTCGCAATTTTGATTATAGAG
GTGGATTTTCTTTTAGAAAAGGAGATCGTGTGAACAAAGGACTATCACTATATTTTGGCTCTAGGCAATCTCCATATTAT
CTTAATTTCTATGAAAAAGATTATGAGCTGGCAAAAAAAGAAGGGCTATCTATTGAAATGGTACATCAAAAATATGGTGT
TAAAAATAGATACGAAATCCGCCTTGCAAACGAAAAAGCCTATCTTTTTGTGGAATATCTATTGTCAACTGGCGAAACCA
TAGAGTGGGTTGCTAGAGAGCTAATTGATACCTCTTTAAAAGTTTTTGATTCTGATAATCAAGGAAATCGTATCAGTTAC
AGCGCCAATTGGCGAATGGTCATTGAAAGTATGCAAGAATTGAAGCTGACCATGAAAGGAGAGAAACCTAATTATGAAAA
ATCATTACGTTGGCTCTCCAATTATCTTGCCCCAACGTTAAAGAAAATTTGGATTATGGATCAAACGTTTGGTACGGATG
AACTAATGACACGTATCCAACAAGCAGAACTGAAAGAAAAGGATCAAGAGGAATTGGTAAAGCTAACCACAACGATTAAA
GAGTTATTAGTACAAGAAGAGGAAGAAGCAGTATCCTCTAAAACTGTATCTGTTACGCAACAAGAAGTGGAGCAGCTGTT
AGCCCAATTTTTATTTGAGTGA

Upstream 100 bases:

>100_bases
AGGAAGTTCTGCTGAAAAGTAGAGCTTCTTTTTTTTGAAATTTGAGTGGTTTCGAATGAACAATACTTTAAATAAAATAT
GTAGTATATAAGGAGGATAA

Downstream 100 bases:

>100_bases
GAGGTGAATGAAATGATTGAGTTATATATTGAAAACCCTAAAGCACGTAAAGGGCGTTGGTTTGAGCTACCTATTCTGTG
GGAAGTTGTACAAGAAAAGT

Product: Cro/CI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKGEQIKKLRQRIGMNQTTLAEKLEISKGYLSKIENNQKKLNSSIINKLQSIFHLTGGILPMEAKIDFLRIRLKIDCPTQ
VITNILKMDPNVFGYKSYGFNHYTETYFFSEIFIFSNPNDINMGVMIELRGQGCREYELILEERKENWNDFFWRLYQDDL
FGKGVLIDTKITRIDIALDEMISCLYKNYDLHELKRKCEQGLVDTTFRNFDYRGGFSFRKGDRVNKGLSLYFGSRQSPYY
LNFYEKDYELAKKEGLSIEMVHQKYGVKNRYEIRLANEKAYLFVEYLLSTGETIEWVARELIDTSLKVFDSDNQGNRISY
SANWRMVIESMQELKLTMKGEKPNYEKSLRWLSNYLAPTLKKIWIMDQTFGTDELMTRIQQAELKEKDQEELVKLTTTIK
ELLVQEEEEAVSSKTVSVTQQEVEQLLAQFLFE

Sequences:

>Translated_433_residues
MKGEQIKKLRQRIGMNQTTLAEKLEISKGYLSKIENNQKKLNSSIINKLQSIFHLTGGILPMEAKIDFLRIRLKIDCPTQ
VITNILKMDPNVFGYKSYGFNHYTETYFFSEIFIFSNPNDINMGVMIELRGQGCREYELILEERKENWNDFFWRLYQDDL
FGKGVLIDTKITRIDIALDEMISCLYKNYDLHELKRKCEQGLVDTTFRNFDYRGGFSFRKGDRVNKGLSLYFGSRQSPYY
LNFYEKDYELAKKEGLSIEMVHQKYGVKNRYEIRLANEKAYLFVEYLLSTGETIEWVARELIDTSLKVFDSDNQGNRISY
SANWRMVIESMQELKLTMKGEKPNYEKSLRWLSNYLAPTLKKIWIMDQTFGTDELMTRIQQAELKEKDQEELVKLTTTIK
ELLVQEEEEAVSSKTVSVTQQEVEQLLAQFLFE
>Mature_433_residues
MKGEQIKKLRQRIGMNQTTLAEKLEISKGYLSKIENNQKKLNSSIINKLQSIFHLTGGILPMEAKIDFLRIRLKIDCPTQ
VITNILKMDPNVFGYKSYGFNHYTETYFFSEIFIFSNPNDINMGVMIELRGQGCREYELILEERKENWNDFFWRLYQDDL
FGKGVLIDTKITRIDIALDEMISCLYKNYDLHELKRKCEQGLVDTTFRNFDYRGGFSFRKGDRVNKGLSLYFGSRQSPYY
LNFYEKDYELAKKEGLSIEMVHQKYGVKNRYEIRLANEKAYLFVEYLLSTGETIEWVARELIDTSLKVFDSDNQGNRISY
SANWRMVIESMQELKLTMKGEKPNYEKSLRWLSNYLAPTLKKIWIMDQTFGTDELMTRIQQAELKEKDQEELVKLTTTIK
ELLVQEEEEAVSSKTVSVTQQEVEQLLAQFLFE

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 51047; Mature: 51047

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEQIKKLRQRIGMNQTTLAEKLEISKGYLSKIENNQKKLNSSIINKLQSIFHLTGGIL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
PMEAKIDFLRIRLKIDCPTQVITNILKMDPNVFGYKSYGFNHYTETYFFSEIFIFSNPND
EEECCEEEEEEEEEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHEEEEEEEEEECCCC
INMGVMIELRGQGCREYELILEERKENWNDFFWRLYQDDLFGKGVLIDTKITRIDIALDE
CCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECEEEEEHHHHHH
MISCLYKNYDLHELKRKCEQGLVDTTFRNFDYRGGFSFRKGDRVNKGLSLYFGSRQSPYY
HHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEE
LNFYEKDYELAKKEGLSIEMVHQKYGVKNRYEIRLANEKAYLFVEYLLSTGETIEWVARE
EEEEHHHHHHHHHCCCEEEEHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHH
LIDTSLKVFDSDNQGNRISYSANWRMVIESMQELKLTMKGEKPNYEKSLRWLSNYLAPTL
HHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHH
KKIWIMDQTFGTDELMTRIQQAELKEKDQEELVKLTTTIKELLVQEEEEAVSSKTVSVTQ
HHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEVEQLLAQFLFE
HHHHHHHHHHHCC
>Mature Secondary Structure
MKGEQIKKLRQRIGMNQTTLAEKLEISKGYLSKIENNQKKLNSSIINKLQSIFHLTGGIL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
PMEAKIDFLRIRLKIDCPTQVITNILKMDPNVFGYKSYGFNHYTETYFFSEIFIFSNPND
EEECCEEEEEEEEEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHEEEEEEEEEECCCC
INMGVMIELRGQGCREYELILEERKENWNDFFWRLYQDDLFGKGVLIDTKITRIDIALDE
CCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECEEEEEHHHHHH
MISCLYKNYDLHELKRKCEQGLVDTTFRNFDYRGGFSFRKGDRVNKGLSLYFGSRQSPYY
HHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEE
LNFYEKDYELAKKEGLSIEMVHQKYGVKNRYEIRLANEKAYLFVEYLLSTGETIEWVARE
EEEEHHHHHHHHHCCCEEEEHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHH
LIDTSLKVFDSDNQGNRISYSANWRMVIESMQELKLTMKGEKPNYEKSLRWLSNYLAPTL
HHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHH
KKIWIMDQTFGTDELMTRIQQAELKEKDQEELVKLTTTIKELLVQEEEEAVSSKTVSVTQ
HHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEVEQLLAQFLFE
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]