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The map label for this gene is potB [H]

Identifier: 116872184

GI number: 116872184

Start: 792055

End: 792864

Strand: Direct

Name: potB [H]

Synonym: lwe0764

Alternate gene names: 116872184

Gene position: 792055-792864 (Clockwise)

Preceding gene: 116872183

Following gene: 116872185

Centisome position: 28.15

GC content: 34.57

Gene sequence:

>810_bases
ATGAATAGACGCACCCGTACAGTTTATCTTGTTCCTTATGTGCTTTGGATTGTACTTTTTGTTGTTGCACCGATTTTATT
AATTGTGTATTATTCTTTTTTTGATGTCGATGGCAATTTTACTGTAGATAATTATATTCACTTTTTTACCCCTGTATACT
TAAAAATGACAGCTAGTTCATTCTGGTATGCGTTTTTAATTACAGTTTTTACATTATTAATTTCCTACCCAACAGCTTAT
CTTTTAACAAAACTGAAACATAAACAATTGTGGTTATTACTCATTATTTTGCCGACTTGGATCAATTTATTGCTTAAAGC
GTATGCCTTTATTGGGATTTTCGGGACGTATGGAGCAGCAAATCAGTTTCTAGAAATACTCGGAATTGGTTCGAAACAGA
TTTTATTCACGGATTTTAGTTTCTTATTTGTATCGACTTATATTTTTATTCCGTTTATGATTTTACCGATTTTTAATGCA
ATTGAGGAAATTAATCCTACGTTAATTCAGGCATCGCGCGATTTAGGTGCATCTAGTTTAACTACATTTAGAAGAGTGAT
ATTCCCGCTCACTGCTGACGGCGTGAAATCAGGGTGCCAGGCTGTTTTTATTCCAGCATTATCCCTCTTTATGATTACTC
GATTAATTGCGGGAAACCGTGTCATTACACTTGGAACAGCGATTGAAGAACATTTCCTCGTGACACAAGACTGGGGAATG
GGTTCGACAATAGGGGTATTTTTAATTATTGCGATGATTTTAATTATGTTTTTAACAGGTTCCAAAAAGAAAAGAGGTGC
GCGTAAATGA

Upstream 100 bases:

>100_bases
TTGGCTTAGCGTTTGAACCAGAGGCAATTCATGTTATGCGTCTTGGGGAAACGGAGGAAGAATTCGATAAACGACTTGAT
AGCTATGATGAGGTGCAGTA

Downstream 100 bases:

>100_bases
AGAAAAGTAAATGGGGTACGATTTATTTAGTGCTTGTTTTTGTGATTTTATATGCGCCGATTTTCTATTTGATTTTTTAT
TCGTTTAACAAAGGTGGAAC

Product: spermidine/putrescine ABC transporter permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNRRTRTVYLVPYVLWIVLFVVAPILLIVYYSFFDVDGNFTVDNYIHFFTPVYLKMTASSFWYAFLITVFTLLISYPTAY
LLTKLKHKQLWLLLIILPTWINLLLKAYAFIGIFGTYGAANQFLEILGIGSKQILFTDFSFLFVSTYIFIPFMILPIFNA
IEEINPTLIQASRDLGASSLTTFRRVIFPLTADGVKSGCQAVFIPALSLFMITRLIAGNRVITLGTAIEEHFLVTQDWGM
GSTIGVFLIIAMILIMFLTGSKKKRGARK

Sequences:

>Translated_269_residues
MNRRTRTVYLVPYVLWIVLFVVAPILLIVYYSFFDVDGNFTVDNYIHFFTPVYLKMTASSFWYAFLITVFTLLISYPTAY
LLTKLKHKQLWLLLIILPTWINLLLKAYAFIGIFGTYGAANQFLEILGIGSKQILFTDFSFLFVSTYIFIPFMILPIFNA
IEEINPTLIQASRDLGASSLTTFRRVIFPLTADGVKSGCQAVFIPALSLFMITRLIAGNRVITLGTAIEEHFLVTQDWGM
GSTIGVFLIIAMILIMFLTGSKKKRGARK
>Mature_269_residues
MNRRTRTVYLVPYVLWIVLFVVAPILLIVYYSFFDVDGNFTVDNYIHFFTPVYLKMTASSFWYAFLITVFTLLISYPTAY
LLTKLKHKQLWLLLIILPTWINLLLKAYAFIGIFGTYGAANQFLEILGIGSKQILFTDFSFLFVSTYIFIPFMILPIFNA
IEEINPTLIQASRDLGASSLTTFRRVIFPLTADGVKSGCQAVFIPALSLFMITRLIAGNRVITLGTAIEEHFLVTQDWGM
GSTIGVFLIIAMILIMFLTGSKKKRGARK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=252, Percent_Identity=37.3015873015873, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=33.6134453781513, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787713, Length=264, Percent_Identity=28.030303030303, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30572; Mature: 30572

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRTRTVYLVPYVLWIVLFVVAPILLIVYYSFFDVDGNFTVDNYIHFFTPVYLKMTASS
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHH
FWYAFLITVFTLLISYPTAYLLTKLKHKQLWLLLIILPTWINLLLKAYAFIGIFGTYGAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NQFLEILGIGSKQILFTDFSFLFVSTYIFIPFMILPIFNAIEEINPTLIQASRDLGASSL
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
TTFRRVIFPLTADGVKSGCQAVFIPALSLFMITRLIAGNRVITLGTAIEEHFLVTQDWGM
HHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHEEEEECCCC
GSTIGVFLIIAMILIMFLTGSKKKRGARK
CCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNRRTRTVYLVPYVLWIVLFVVAPILLIVYYSFFDVDGNFTVDNYIHFFTPVYLKMTASS
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHH
FWYAFLITVFTLLISYPTAYLLTKLKHKQLWLLLIILPTWINLLLKAYAFIGIFGTYGAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NQFLEILGIGSKQILFTDFSFLFVSTYIFIPFMILPIFNAIEEINPTLIQASRDLGASSL
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
TTFRRVIFPLTADGVKSGCQAVFIPALSLFMITRLIAGNRVITLGTAIEEHFLVTQDWGM
HHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHEEEEECCCC
GSTIGVFLIIAMILIMFLTGSKKKRGARK
CCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]