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The map label for this gene is azoR2 [H]

Identifier: 116872165

GI number: 116872165

Start: 768926

End: 769561

Strand: Direct

Name: azoR2 [H]

Synonym: lwe0745

Alternate gene names: 116872165

Gene position: 768926-769561 (Clockwise)

Preceding gene: 116872158

Following gene: 116872166

Centisome position: 27.32

GC content: 37.11

Gene sequence:

>636_bases
ATGTCAAAAGTATTATTTATCAAAGCGTCACCGCTTCCTAATGAAGTATCAAGAAGTTCACAAGTAGCAGCGACTTTTAT
GGAAGAATATAAAGCGAAAAATCCTTCTGATACAGTAGAAGAATTGGTTTTATATAATACAGAAGTACCTTTACTCGATT
TAGAATTAATGACAGCTGGAAGAGAGCTACAAGCTGGTAAAGCCTTCGCAGATTTAGACCCAGATGTACAAAAAAGATTA
AATGCGTATAATGCGCTTACAGATCAATTCCTAGCAGCAGACAAATTTGTTTTTGTTTTCCCACTTTGGAACCTTGGAAT
TCCACCGCTTTTAAAGGCATATGTGGATACATTTGTTATTGCAGGTAAATCTTTCCGTTATACAGAACATGGACCAGAAG
CACTTTTAAAAGGTAAAAAAGCGATATTGATTCATGGTAGCGGTGGTATTTATTCAGCTGGAGATGCAAGTTCATTCACT
CACGGAGAACCGTATTTACGTACTATTTTACAATTTATTGGTATTGAAGTAGTACCGTCCATCTTTGTAGAAGGGATTGA
CCACAATCCTAGCAAAGAAGCGGAAATCGTTGCAGCTGCTAAAACGGTAGCGCGTGAAAGTGCTACAGATTTTTAA

Upstream 100 bases:

>100_bases
ATGAAAATGTCAATGATTATTTAATAAAAATCAAATTGACTACCTTTCTATATAGTACTATTATATAGAAGGAACAAAAA
AAACTGATGAGGTGTTCATA

Downstream 100 bases:

>100_bases
AAAGTTTGTGAAAAGTTAGTTTGTAGTTTTAAATGTTAAGATAGTACTATCAACTGTCTAGACCAATAAAAACATATAAA
AAGATTGCTAATCCCTTTAT

Product: acyl-carrier protein phosphodiesterase, putative

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSKVLFIKASPLPNEVSRSSQVAATFMEEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFADLDPDVQKRL
NAYNALTDQFLAADKFVFVFPLWNLGIPPLLKAYVDTFVIAGKSFRYTEHGPEALLKGKKAILIHGSGGIYSAGDASSFT
HGEPYLRTILQFIGIEVVPSIFVEGIDHNPSKEAEIVAAAKTVARESATDF

Sequences:

>Translated_211_residues
MSKVLFIKASPLPNEVSRSSQVAATFMEEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFADLDPDVQKRL
NAYNALTDQFLAADKFVFVFPLWNLGIPPLLKAYVDTFVIAGKSFRYTEHGPEALLKGKKAILIHGSGGIYSAGDASSFT
HGEPYLRTILQFIGIEVVPSIFVEGIDHNPSKEAEIVAAAKTVARESATDF
>Mature_210_residues
SKVLFIKASPLPNEVSRSSQVAATFMEEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFADLDPDVQKRLN
AYNALTDQFLAADKFVFVFPLWNLGIPPLLKAYVDTFVIAGKSFRYTEHGPEALLKGKKAILIHGSGGIYSAGDASSFTH
GEPYLRTILQFIGIEVVPSIFVEGIDHNPSKEAEIVAAAKTVARESATDF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=201, Percent_Identity=39.3034825870647, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 23083; Mature: 22951

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVLFIKASPLPNEVSRSSQVAATFMEEYKAKNPSDTVEELVLYNTEVPLLDLELMTAG
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHCC
RELQAGKAFADLDPDVQKRLNAYNALTDQFLAADKFVFVFPLWNLGIPPLLKAYVDTFVI
CHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCHHHHHHHHHHHHH
AGKSFRYTEHGPEALLKGKKAILIHGSGGIYSAGDASSFTHGEPYLRTILQFIGIEVVPS
CCCCCCCCCCCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IFVEGIDHNPSKEAEIVAAAKTVARESATDF
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKVLFIKASPLPNEVSRSSQVAATFMEEYKAKNPSDTVEELVLYNTEVPLLDLELMTAG
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHCC
RELQAGKAFADLDPDVQKRLNAYNALTDQFLAADKFVFVFPLWNLGIPPLLKAYVDTFVI
CHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCHHHHHHHHHHHHH
AGKSFRYTEHGPEALLKGKKAILIHGSGGIYSAGDASSFTHGEPYLRTILQFIGIEVVPS
CCCCCCCCCCCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IFVEGIDHNPSKEAEIVAAAKTVARESATDF
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]