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The map label for this gene is ydaP [H]

Identifier: 116872111

GI number: 116872111

Start: 715801

End: 717531

Strand: Direct

Name: ydaP [H]

Synonym: lwe0691

Alternate gene names: 116872111

Gene position: 715801-717531 (Clockwise)

Preceding gene: 116872109

Following gene: 116872112

Centisome position: 25.44

GC content: 39.05

Gene sequence:

>1731_bases
ATGAAAAAAGTAAAAGCAAGTGAAACACTCGTACAAACACTGAAAAATTGGGGTATTGACCACGTTTACGGCTTACCCGG
CGATTCGATTGATACAGTAGTCGACGCTTTAAGAAAAGAACAAGAGGATATTGAATTTATCCATGTTCGACACGAAGAAG
TAGCCGCCTTATCTGCTGCAGCATATGCCAAACTAACTGGTAAAATTGGTGTTGCGTTATCCATTGGTGGACCAGGAGCA
ATCCATCTTTTAAATGGAATGTATGATGCAAAAATGGATCATGTACCAATGCTTGTTCTAGTTGGTCAAGTAACAACAGA
CGTCTTAAATACTGGTTTTTTCCAAGAAGTCAATTTACCAGCAGTTTTTGAAGATGTTGCTGTTTATAACAAACAAATTG
ATAATCCTGAAACGCTAGCAGATGTGGTTGATGAAGCAATTCGTACAGCCTATAAAGAAAAAGGTGTCGCAGTTTTAACC
ATTTCAAATGATATTCCAGCTCAAGCAATTAAAGCAAGTTTAGAAGCAGAAACAGTAAAATTTGAACAAGAAAATCCAAA
ACTAAACGAAGCAAACATTCAAGAAGCTGTGGCATTAATTGATAAAGCGGAAAAACCAGTTATCTTAGCAGGTTTAGGAA
CCAAACATGCTGGTCCAGAATTAATCGCCTTTGCAGAAAAAGCAAAAATCCCGATTATTCATTCTTTACCAGCAAAAACA
ATTATTCCAGATGATCACCCAAACGCACTCGGAAATCTTGGGAAAATAGGAACTAAACCAGCTTATGAAGCTATGCAAGA
AACAGATTTACTTTTAATGTTTGGAAACGACTATCCATACAGCGACTATTTACCTAAAAAAGCCGCGTGTGTCCAAATTG
ACATTGACCCAGCAAAAATCAGCAAACGTTATCAAGCGACAGTTGGCTTAGTTGGCGATGCAGCCGAAATTATTAGCAAT
TTAACAGCAAAAATTGCACCTGTGGAAGAACGTAAATTCCTCCAAGCATGTCAAGAAAATATGCAAGAGTGGTGGAAATG
GTTAGAAGAAGATATGACAAAAACAACTGATCCAATCGCTCCAGAAGTCGTTATGGCAAATATTCAAAAAATCGCTGACA
AAGATGCTGTTTTCTCTATCGATGTAGGAACAGCAACCGTTTGGAGTACTCGTTATTTACATTTAACCCCAGAAAATGAC
TTTATCGTTTCAGCGTGGTTAGGTACTATGGGTTGCGGTTTACCAGGAGCTATCGCCGCCAAAAAAGCATTCCCAGACCG
CCAAGCAATTGCCATCGTAGGTGATGGAGGCTTTTCTATGGTTATGCAAGATTTCGCAACTGCTGTAGGTCTCAATATGC
CAATGATTGTCGTCGTGTTAAACAACCAACAACTATCCTTTATTAAATATGAACAACAATCTGCTGGCGAACTAAATTAT
GCCATTGACCTACCAGATATCAATTACGCAAAATTCGCCGAAAGTTGTGGCGGAATAGGCTTCCGAGTAGAAAAAATGGC
CGACCTAGAAAGCGCCTTTGAAAACGCCAAACTAGCAACCAAACCAGTCATCATAGATGTTTCTGTAGATAGTGCAGCCG
CTCCACTTCCAGGGAAAATCGTTATGGATGAAGCACTCGGTTACACTAAATTCGAAATTCAATCAGTTCTAGAAGACCAT
CGTTTCGCAAAAATGCCACCACTTAAAACAATTTTGCGCAGATTTTTATAA

Upstream 100 bases:

>100_bases
GAATTGTTCGTCAATTCTCTTTTATGAATACTCTTTCGAAAACAGGGTAAACGAAAACTATTGGACATTAGAGAAAACAT
CCATGGGAGGAGAAAATAAA

Downstream 100 bases:

>100_bases
AAAATAAATAAAAAAATAATGATATCACCCATTTTACACCGATATTCTCTCGTTATTATAATGTTTTTTTATTAAAACTA
ATTATTTTACAATAAAATAT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEDIEFIHVRHEEVAALSAAAYAKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLVGQVTTDVLNTGFFQEVNLPAVFEDVAVYNKQIDNPETLADVVDEAIRTAYKEKGVAVLT
ISNDIPAQAIKASLEAETVKFEQENPKLNEANIQEAVALIDKAEKPVILAGLGTKHAGPELIAFAEKAKIPIIHSLPAKT
IIPDDHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKAACVQIDIDPAKISKRYQATVGLVGDAAEIISN
LTAKIAPVEERKFLQACQENMQEWWKWLEEDMTKTTDPIAPEVVMANIQKIADKDAVFSIDVGTATVWSTRYLHLTPEND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFATAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLESAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL

Sequences:

>Translated_576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEDIEFIHVRHEEVAALSAAAYAKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLVGQVTTDVLNTGFFQEVNLPAVFEDVAVYNKQIDNPETLADVVDEAIRTAYKEKGVAVLT
ISNDIPAQAIKASLEAETVKFEQENPKLNEANIQEAVALIDKAEKPVILAGLGTKHAGPELIAFAEKAKIPIIHSLPAKT
IIPDDHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKAACVQIDIDPAKISKRYQATVGLVGDAAEIISN
LTAKIAPVEERKFLQACQENMQEWWKWLEEDMTKTTDPIAPEVVMANIQKIADKDAVFSIDVGTATVWSTRYLHLTPEND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFATAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLESAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL
>Mature_576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEDIEFIHVRHEEVAALSAAAYAKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLVGQVTTDVLNTGFFQEVNLPAVFEDVAVYNKQIDNPETLADVVDEAIRTAYKEKGVAVLT
ISNDIPAQAIKASLEAETVKFEQENPKLNEANIQEAVALIDKAEKPVILAGLGTKHAGPELIAFAEKAKIPIIHSLPAKT
IIPDDHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKAACVQIDIDPAKISKRYQATVGLVGDAAEIISN
LTAKIAPVEERKFLQACQENMQEWWKWLEEDMTKTTDPIAPEVVMANIQKIADKDAVFSIDVGTATVWSTRYLHLTPEND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFATAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLESAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=25, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1787096, Length=563, Percent_Identity=33.214920071048, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=29.2173913043478, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790104, Length=540, Percent_Identity=28.7037037037037, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=26.1180679785331, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=25.7142857142857, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=24.5080500894454, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=24.5080500894454, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=556, Percent_Identity=22.841726618705, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=572, Percent_Identity=27.2727272727273, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=543, Percent_Identity=23.572744014733, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320588, Length=577, Percent_Identity=20.7972270363951, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19922626, Length=588, Percent_Identity=24.6598639455782, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 62766; Mature: 62766

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEDIEFIHVRHEEVAALSAA
CCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
AYAKLTGKIGVALSIGGPGAIHLLNGMYDAKMDHVPMLVLVGQVTTDVLNTGFFQEVNLP
HHHHCCCCEEEEEEECCCCHHHHHCCHHHCCCCCCCCEEEHHHHHHHHHCCCCCEECCCC
AVFEDVAVYNKQIDNPETLADVVDEAIRTAYKEKGVAVLTISNDIPAQAIKASLEAETVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHEE
FEQENPKLNEANIQEAVALIDKAEKPVILAGLGTKHAGPELIAFAEKAKIPIIHSLPAKT
EECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHEEEHHHCCCCEEECCCCCC
IIPDDHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKAACVQIDIDPAKI
CCCCCCCHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHH
SKRYQATVGLVGDAAEIISNLTAKIAPVEERKFLQACQENMQEWWKWLEEDMTKTTDPIA
HHHHHHHHEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEVVMANIQKIADKDAVFSIDVGTATVWSTRYLHLTPENDFIVSAWLGTMGCGLPGAIAA
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCEEEEEEHHHHCCCCCCHHHH
KKAFPDRQAIAIVGDGGFSMVMQDFATAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
HHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEE
AIDLPDINYAKFAESCGGIGFRVEKMADLESAFENAKLATKPVIIDVSVDSAAAPLPGKI
EEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCE
VMDEALGYTKFEIQSVLEDHRFAKMPPLKTILRRFL
EEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEDIEFIHVRHEEVAALSAA
CCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
AYAKLTGKIGVALSIGGPGAIHLLNGMYDAKMDHVPMLVLVGQVTTDVLNTGFFQEVNLP
HHHHCCCCEEEEEEECCCCHHHHHCCHHHCCCCCCCCEEEHHHHHHHHHCCCCCEECCCC
AVFEDVAVYNKQIDNPETLADVVDEAIRTAYKEKGVAVLTISNDIPAQAIKASLEAETVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHEE
FEQENPKLNEANIQEAVALIDKAEKPVILAGLGTKHAGPELIAFAEKAKIPIIHSLPAKT
EECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHEEEHHHCCCCEEECCCCCC
IIPDDHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKAACVQIDIDPAKI
CCCCCCCHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHH
SKRYQATVGLVGDAAEIISNLTAKIAPVEERKFLQACQENMQEWWKWLEEDMTKTTDPIA
HHHHHHHHEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEVVMANIQKIADKDAVFSIDVGTATVWSTRYLHLTPENDFIVSAWLGTMGCGLPGAIAA
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCEEEEEEHHHHCCCCCCHHHH
KKAFPDRQAIAIVGDGGFSMVMQDFATAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
HHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEE
AIDLPDINYAKFAESCGGIGFRVEKMADLESAFENAKLATKPVIIDVSVDSAAAPLPGKI
EEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCE
VMDEALGYTKFEIQSVLEDHRFAKMPPLKTILRRFL
EEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]