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The map label for this gene is 116871899

Identifier: 116871899

GI number: 116871899

Start: 514825

End: 516033

Strand: Direct

Name: 116871899

Synonym: lwe0479

Alternate gene names: NA

Gene position: 514825-516033 (Clockwise)

Preceding gene: 116871898

Following gene: 116871900

Centisome position: 18.29

GC content: 31.02

Gene sequence:

>1209_bases
ATGAAAAATTTAGAAAAAAGAGTGCCAATAATCCGGTTTAAAGGCTTTTCTGAAGCTTGGGAACAGCGTAAGGTTTTAGA
TTATGCAATACACACTTATGGTGGAGGAACTCCTAAAACTAATGTGCCTGAATATTGGAGCGGAGAAATCCCTTGGATAC
AAAGTAGTGATTTGTCAATAAGTAATTTATTTAATATAATCCCTAAAAAACATATTACAGAATTAGCCATAAAAAGTTCA
GCGACTAAATTTATTCCAGCTAATTCAATTGCAATTGTTTCACGAGTAGGCGTAGGAAAACTAGTATTTATGCCCTTTGA
ATACACAACTAGTCAGGATTTCTTGTCTCTTTCTAACTTACAAGTAGATTCTAATTTTGGAGTTTATTCTATATATATGA
TGCTTCAAAGAGAATTAAACAATATTCAAGGAACTTCAATTAAAGGAATCACTAAATCAGACTTGTTAGAGAAAAAAATT
AACAAACCATCAAGTAGAGAAGAACAACAAAAAATAGGCTCTTTCTTCAAACAACTCGATAATACTATCGCTCTTCATCA
ACGTAAGTTAGAGGCTTTAAAGCTGATGAAGAAAGGGTTATTACAGCAAATGTTTCCCAAAAGCGAAGCGGATATCCCCA
AGATACGGTTTGCTGATTTTGATGGTAAATGGGAACAGCGTAAGTTGGGAGATGTTTTTAATGAAAGATCAGAACGAAGT
GCGGACGGAGAGCTAATTTCTGTAACTATTAATTCAGGAGTAATTAAAGCTTCTAAATTAGAGAAAAAAGACAACTCTAG
TTTTGATAAATCTAATTATAAAGTTGTAAAAAAAGGTGATATAGCATATAACTCTATGCGTATGTGGCAAGGGGCGAGCG
GGTATTCATCTTATAACGGAATACTCAGCCCAGCTTATACTGTAATTTATCCAATAAAAAATATTAATGCAATGTTTATA
GCTTATATATTTAAAAAAAATGATATGATTCAAACTTTTCAGCGTAACTCACAAGGATTAACTTCAGATACATGGAATTT
AAAATTCCCATCTTTATCTAAAATTAAGATTAAAATACCAACGAATGAAGAGCAAATAAAAATTACTAACTTATTACGAA
AATTAGAATACACTTCTACATTTCATCAAAACAAAATAGAACGACTAAAAAAGTTAAAAAAAGCTTATTTGCAAACTATG
TTCATTTAA

Upstream 100 bases:

>100_bases
ATATAAAAAAAGCTGAAACTGATTTTCTTGGTTTATTAGATGAGTTAGCTGTTACTACGGATACAAAGGATATAATTGAA
GCTACTAAGGCGGTATTTCG

Downstream 100 bases:

>100_bases
TTAAAACATTTACACTTAAACTAACTCCTAAGATAATAATAAAAGGTGATCAACTCATCTATTTATTATCTTAGGAGGTT
TTTTATGAAAGCGAAAAAGA

Product: type I restriction endonuclease S subunit

Products: NA

Alternate protein names: S.MjaXP; Type I restriction enzyme MjaXP specificity protein; S protein [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKNLEKRVPIIRFKGFSEAWEQRKVLDYAIHTYGGGTPKTNVPEYWSGEIPWIQSSDLSISNLFNIIPKKHITELAIKSS
ATKFIPANSIAIVSRVGVGKLVFMPFEYTTSQDFLSLSNLQVDSNFGVYSIYMMLQRELNNIQGTSIKGITKSDLLEKKI
NKPSSREEQQKIGSFFKQLDNTIALHQRKLEALKLMKKGLLQQMFPKSEADIPKIRFADFDGKWEQRKLGDVFNERSERS
ADGELISVTINSGVIKASKLEKKDNSSFDKSNYKVVKKGDIAYNSMRMWQGASGYSSYNGILSPAYTVIYPIKNINAMFI
AYIFKKNDMIQTFQRNSQGLTSDTWNLKFPSLSKIKIKIPTNEEQIKITNLLRKLEYTSTFHQNKIERLKKLKKAYLQTM
FI

Sequences:

>Translated_402_residues
MKNLEKRVPIIRFKGFSEAWEQRKVLDYAIHTYGGGTPKTNVPEYWSGEIPWIQSSDLSISNLFNIIPKKHITELAIKSS
ATKFIPANSIAIVSRVGVGKLVFMPFEYTTSQDFLSLSNLQVDSNFGVYSIYMMLQRELNNIQGTSIKGITKSDLLEKKI
NKPSSREEQQKIGSFFKQLDNTIALHQRKLEALKLMKKGLLQQMFPKSEADIPKIRFADFDGKWEQRKLGDVFNERSERS
ADGELISVTINSGVIKASKLEKKDNSSFDKSNYKVVKKGDIAYNSMRMWQGASGYSSYNGILSPAYTVIYPIKNINAMFI
AYIFKKNDMIQTFQRNSQGLTSDTWNLKFPSLSKIKIKIPTNEEQIKITNLLRKLEYTSTFHQNKIERLKKLKKAYLQTM
FI
>Mature_402_residues
MKNLEKRVPIIRFKGFSEAWEQRKVLDYAIHTYGGGTPKTNVPEYWSGEIPWIQSSDLSISNLFNIIPKKHITELAIKSS
ATKFIPANSIAIVSRVGVGKLVFMPFEYTTSQDFLSLSNLQVDSNFGVYSIYMMLQRELNNIQGTSIKGITKSDLLEKKI
NKPSSREEQQKIGSFFKQLDNTIALHQRKLEALKLMKKGLLQQMFPKSEADIPKIRFADFDGKWEQRKLGDVFNERSERS
ADGELISVTINSGVIKASKLEKKDNSSFDKSNYKVVKKGDIAYNSMRMWQGASGYSSYNGILSPAYTVIYPIKNINAMFI
AYIFKKNDMIQTFQRNSQGLTSDTWNLKFPSLSKIKIKIPTNEEQIKITNLLRKLEYTSTFHQNKIERLKKLKKAYLQTM
FI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46133; Mature: 46133

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLEKRVPIIRFKGFSEAWEQRKVLDYAIHTYGGGTPKTNVPEYWSGEIPWIQSSDLSI
CCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCH
SNLFNIIPKKHITELAIKSSATKFIPANSIAIVSRVGVGKLVFMPFEYTTSQDFLSLSNL
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCHHHHHHHCE
QVDSNFGVYSIYMMLQRELNNIQGTSIKGITKSDLLEKKINKPSSREEQQKIGSFFKQLD
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NTIALHQRKLEALKLMKKGLLQQMFPKSEADIPKIRFADFDGKWEQRKLGDVFNERSERS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
ADGELISVTINSGVIKASKLEKKDNSSFDKSNYKVVKKGDIAYNSMRMWQGASGYSSYNG
CCCCEEEEEECCCEEEHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCCCCCCCC
ILSPAYTVIYPIKNINAMFIAYIFKKNDMIQTFQRNSQGLTSDTWNLKFPSLSKIKIKIP
CCCCCEEEEEECCCCCEEEEEEEHHCCCHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEC
TNEEQIKITNLLRKLEYTSTFHQNKIERLKKLKKAYLQTMFI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLEKRVPIIRFKGFSEAWEQRKVLDYAIHTYGGGTPKTNVPEYWSGEIPWIQSSDLSI
CCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCH
SNLFNIIPKKHITELAIKSSATKFIPANSIAIVSRVGVGKLVFMPFEYTTSQDFLSLSNL
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCHHHHHHHCE
QVDSNFGVYSIYMMLQRELNNIQGTSIKGITKSDLLEKKINKPSSREEQQKIGSFFKQLD
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NTIALHQRKLEALKLMKKGLLQQMFPKSEADIPKIRFADFDGKWEQRKLGDVFNERSERS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
ADGELISVTINSGVIKASKLEKKDNSSFDKSNYKVVKKGDIAYNSMRMWQGASGYSSYNG
CCCCEEEEEECCCEEEHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCCCCCCCC
ILSPAYTVIYPIKNINAMFIAYIFKKNDMIQTFQRNSQGLTSDTWNLKFPSLSKIKIKIP
CCCCCEEEEEECCCCCEEEEEEEHHCCCHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEC
TNEEQIKITNLLRKLEYTSTFHQNKIERLKKLKKAYLQTMFI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]