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The map label for this gene is gmuD [H]

Identifier: 116871748

GI number: 116871748

Start: 352201

End: 353637

Strand: Direct

Name: gmuD [H]

Synonym: lwe0328

Alternate gene names: 116871748

Gene position: 352201-353637 (Clockwise)

Preceding gene: 116871743

Following gene: 116871749

Centisome position: 12.52

GC content: 36.67

Gene sequence:

>1437_bases
ATGAAAGAATTATTAACGTTTCCAAAGGATTTTTGGTGGGGCTCTGCGTGGTCTGCTGAACAAGCAGAAGGTCGCGGCGA
CACTGGTAAGGCAAAAACCGTTTGGGAGCATTGGTTTGAAACAGAACCGAATCGCTTTTATGAAGGTGTAGGTTCAGAAA
TTACAACAGATCATTTTAATCGGTATAAAGAAGATGTACAGTGGATGAAGAAAACAGGACAAAATTCATTCCGTATTTCG
ATTTCATGGGCAAGAATGTTTCCAAATGATGGTGTCGGGGAAGTGAACCAGAAAGCTATTGCGTTTTATCGTGATTTACT
TACTGAGATGAATGAAAACGGTATCAAACCATTTGCGAATTTATATCATTTCGATATGCCTGTTGCACTTCAAGATGCTG
GTGGCTGGGAGTCAAGAGCAGTTGTTGATGCCTACGTTCATTTTGCAGATACATGTTTTAAAGAATTCGGCGATTTAGTT
TATCACTGGTTTACATTTAATGAACCACTTGGACCAATTCTTGGCGGCTATTTAGAAGATTTCCATTATCCAAATCAAAT
CGACTTTAAACGAGGTGCGCAAGCTGGTTTTAACACTATTTTGGCACATGCTTTAGCTATTAAAGCTTTCAAAAAATTAA
ACTTATCATCTAAAATCGGTGTTATTTTAAACCTTAGTCCAACATATCCACGTAGCCAAAATGAATACGATTTAGAAGCT
GCAGAAATATGTGATGCTTTTTATACGAGAAGTTTCTTAGATCCAATGGTTAAAGGAACTTTCCCAGAAAAATTAGTAGA
AATTATGCGTGAATACGATCAAATGCCGGAATACACCGAAGAAGATTTGACAATTATTAAAGAAAATACGGCGCAAATCC
TTGGTTTGAACTATTATGAACCAAGACGGGTAAAAGCTCGTTTAACGGCTATTAATGAAAAAAGTCCATTTTTACCAGAT
TGGTTTTTTGAACCGCATAATATGCCTGGTAAACGAATGAATATTTATCGCGGTTGGGAAATTTATGAACGCGGAATTTA
TGATTTATGTATTGATATTCGCGATAATTACGGCAACATCGAATCATTTATTTCTGAAAATGGAATGGGTGTAGCAAATG
AAGAGCGTTTCTTGAATGAGGAAGGCCAAATTCAAGATGATTATCGTATTCAATTTGTCAAAGATCATCTGGCATATGTA
CATCAAGCAATTGCAGAAGGTTGCGATATTAAAGGGTATCACTTATGGACATTTATTGATTGCTGGTCTTGGATTAATGC
CTATAAAAACCGTTACGGACTCGTTTCGCTTGATGTCGAAACTGGCAAACGTACGATGAAGAAAAGTGGCGAATTTTACA
AAAAAATGAGCGACATGAATGGTTTTGAATATGAAACAAGCAAACTAGTTGGTACGAAAAGGGAGGAAAACAACTAA

Upstream 100 bases:

>100_bases
AGGTATCCCTATAAACAAAAGTATATATCTTTTAATAAAAGTATTGTTTTTTAAATAAATGTATGCTATATTTGTTTTGA
CAAAAAGGAGGCCATAAAAA

Downstream 100 bases:

>100_bases
TGGCTGAATCGAAAAAGAAGTCGATTGTTAATGGATTTATTAATGTAGCACAGCGTCTTGGTGGACAGATTCATTTGCGC
TCATTACGTGATGCTTTTGC

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNQKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESRAVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQAGFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGEFYKKMSDMNGFEYETSKLVGTKREENN

Sequences:

>Translated_478_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNQKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESRAVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQAGFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGEFYKKMSDMNGFEYETSKLVGTKREENN
>Mature_478_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNQKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESRAVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQAGFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGEFYKKMSDMNGFEYETSKLVGTKREENN

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=486, Percent_Identity=29.4238683127572, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI13273313, Length=481, Percent_Identity=28.6902286902287, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI110681710, Length=481, Percent_Identity=29.3139293139293, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI28376633, Length=482, Percent_Identity=25.9336099585062, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=26.3803680981595, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789070, Length=492, Percent_Identity=31.9105691056911, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI2367174, Length=490, Percent_Identity=31.4285714285714, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI2367270, Length=475, Percent_Identity=31.1578947368421, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17552856, Length=477, Percent_Identity=31.0272536687631, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17539390, Length=468, Percent_Identity=30.3418803418803, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21356577, Length=483, Percent_Identity=25.6728778467909, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55808; Mature: 55808

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFN
CCCHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
RYKEDVQWMKKTGQNSFRISISWARMFPNDGVGEVNQKAIAFYRDLLTEMNENGIKPFAN
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
LYHFDMPVALQDAGGWESRAVVDAYVHFADTCFKEFGDLVYHWFTFNEPLGPILGGYLED
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
FHYPNQIDFKRGAQAGFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHH
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCC
PRRVKARLTAINEKSPFLPDWFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNI
CHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCHHHEEEEECCCCCHH
ESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYVHQAIAEGCDIKGYHLWTFID
HHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
CWSWINAYKNRYGLVSLDVETGKRTMKKSGEFYKKMSDMNGFEYETSKLVGTKREENN
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCC
>Mature Secondary Structure
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFN
CCCHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
RYKEDVQWMKKTGQNSFRISISWARMFPNDGVGEVNQKAIAFYRDLLTEMNENGIKPFAN
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
LYHFDMPVALQDAGGWESRAVVDAYVHFADTCFKEFGDLVYHWFTFNEPLGPILGGYLED
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
FHYPNQIDFKRGAQAGFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHH
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCC
PRRVKARLTAINEKSPFLPDWFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNI
CHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCHHHEEEEECCCCCHH
ESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYVHQAIAEGCDIKGYHLWTFID
HHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
CWSWINAYKNRYGLVSLDVETGKRTMKKSGEFYKKMSDMNGFEYETSKLVGTKREENN
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]