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The map label for this gene is bglH [H]

Identifier: 116871650

GI number: 116871650

Start: 244895

End: 246355

Strand: Direct

Name: bglH [H]

Synonym: lwe0230

Alternate gene names: 116871650

Gene position: 244895-246355 (Clockwise)

Preceding gene: 116871649

Following gene: 116871651

Centisome position: 8.7

GC content: 39.29

Gene sequence:

>1461_bases
ATGACTGAAGTGAAAAAACAATTTCCAAAAGGATTTTTATGGGGTGGAGCAACTGCTGCAAACCAAGTTGAAGGCGCTTA
CGATTTAGATGGTAAAGGCCTTTCCACAGCAGATATGGTAAAATTCATTCCGAAAGAAGAACGCACACAAGACCACGCAT
TAGATGTATCTAAAGCTGAAATCGAGGCAATTATCGCAGGGAAAGTAGATGCAAGATTCCCTAAACGTGAAGGCGTTGAT
TTTTATCATCATTATAAAGAAGACATTGCCTTATTTGCCGAAATGGGCTTTAAAACTTTCCGTCTATCATTAAACTGGGC
GCGAATTTTCCCGAATGGTGATGACAAAGAGCCAAATGAAAAAGGCCTTGAATTTTATGATAAAGTGTTTGATGAATTAT
TAAAATATGACATCGAGCCACTTGTGACACTTTCTCACTATGAAACGCCTTTAAACCTTACTTTAAAATATAACGGTTGG
GCTGATCGTCGTGTTATCGGATTTTTCACGAATTATGCAGAAACAGTTTTTAAACGTTATAAAAATAAAGTAAAATATTG
GTTAACTTTTAACGAAATCAATGTTATTTCACTTAGCGCATACACTGGTGGCGGGGTGCTTTTAGAGGATGCGAAAAACC
CGCTGGAAGTTTCTTACCAAGCGGCTCATCACCAATTTGTAGCTAGTGCACTTGCGACAAAATTAGCTCATGAAATTATT
CCAGGCGCACAAGTCGGTTGTATGTTAGCTCGTATGGCAACATATCCTGCGACGAATAATCCTGATGACATCTTAAAAGC
GCAATATGAAAATCAACAAAACCTATTCTTCACAGATGTTCACGCTCGCGGAGAATATCCAAGCTTCATGAACCGTTTCT
TCCAAGAGAACGACATTCAAATCGTAAAAGAAATTGGCGATGATGAAATTCTAAAAGCACATACCGTTGATTTCATCTCA
TTCAGTTATTACATGTCCTTGTCCGCAACAGCTAGTCCAGAAGGCGACCGCTCTGCTGGGAACTTAATGGGCGGCGTGAA
AAATGAATACCTTGAATCATCTGATTGGGGTTGGCAAATTGATCCTAAAGGTTTACGCTGGACATTAAACGATTTGTATA
GTCGTTATGAATTACCACTTTTCATTGTAGAAAATGGTTTAGGTGCATATGATAAAGTGGAAGAAGATGGCAAAATCCAT
GATGATTACCGAATTGAATACTTGCGCAAACACATCGAACAAATGAAAGAAGCAATTGCTGATGGCGTAGACCTAATTGG
CTACACAAGCTGGGGCCCAATCGACCTAGTAAGTGCCTCCACAAGCGAAATGTCTAAACGCTACGGCTTCATCTACGTAG
ACCAAGATGACTGGGGCAAAGGAACACTAGAACGTTCTCGCAAAGATTCCTTCTTCTGGTATAAAAAAGTAATTGAAACA
AATGGTGAAGATTTAGACTAA

Upstream 100 bases:

>100_bases
AAGCTCTTTTATTATTAAACAAAATTTCTTCTTGACCTGTAATGCATTACATAGATTAAACTTGAAAAGTAGAAGAAATT
AGCCGGAAGGGGCGAAAGAA

Downstream 100 bases:

>100_bases
TAGAAAAATCGCAGTCCCACTTAGGTGGGGCTGTTTTTTTTGCGAATCTAAAAGTGGATTATATATCATTTACGGATAAT
AAAGTTTGAAAATACCAAAA

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTEVKKQFPKGFLWGGATAANQVEGAYDLDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVDARFPKREGVD
FYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGW
ADRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLEVSYQAAHHQFVASALATKLAHEII
PGAQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIQIVKEIGDDEILKAHTVDFIS
FSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDKVEEDGKIH
DDYRIEYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
NGEDLD

Sequences:

>Translated_486_residues
MTEVKKQFPKGFLWGGATAANQVEGAYDLDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVDARFPKREGVD
FYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGW
ADRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLEVSYQAAHHQFVASALATKLAHEII
PGAQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIQIVKEIGDDEILKAHTVDFIS
FSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDKVEEDGKIH
DDYRIEYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
NGEDLD
>Mature_485_residues
TEVKKQFPKGFLWGGATAANQVEGAYDLDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVDARFPKREGVDF
YHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWA
DRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLEVSYQAAHHQFVASALATKLAHEIIP
GAQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIQIVKEIGDDEILKAHTVDFISF
SYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDKVEEDGKIHD
DYRIEYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIETN
GEDLD

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=498, Percent_Identity=28.9156626506024, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI110681710, Length=502, Percent_Identity=30.4780876494024, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI13273313, Length=495, Percent_Identity=26.8686868686869, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI28376633, Length=486, Percent_Identity=27.9835390946502, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=29.4238683127572, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI1789070, Length=480, Percent_Identity=55.625, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI2367270, Length=481, Percent_Identity=55.7172557172557, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI2367174, Length=491, Percent_Identity=52.9531568228106, Blast_Score=519, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17552856, Length=495, Percent_Identity=28.0808080808081, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17539390, Length=456, Percent_Identity=28.2894736842105, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21356577, Length=479, Percent_Identity=26.5135699373695, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55606; Mature: 55475

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVKKQFPKGFLWGGATAANQVEGAYDLDGKGLSTADMVKFIPKEERTQDHALDVSKAE
CCCHHHHCCCCEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCHHH
IEAIIAGKVDARFPKREGVDFYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNE
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCH
KGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWADRRVIGFFTNYAETVFKRY
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLEVSYQAAHHQFVASALATKLAHEII
HCCEEEEEEECCEEEEEEEEECCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
PGAQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIQ
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHH
IVKEIGDDEILKAHTVDFISFSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQI
HHHHCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHCCCCCCCEEE
DPKGLRWTLNDLYSRYELPLFIVENGLGAYDKVEEDGKIHDDYRIEYLRKHIEQMKEAIA
CCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
CCCCEEEECCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHC
NGEDLD
CCCCCC
>Mature Secondary Structure 
TEVKKQFPKGFLWGGATAANQVEGAYDLDGKGLSTADMVKFIPKEERTQDHALDVSKAE
CCHHHHCCCCEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCHHH
IEAIIAGKVDARFPKREGVDFYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNE
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCH
KGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWADRRVIGFFTNYAETVFKRY
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLEVSYQAAHHQFVASALATKLAHEII
HCCEEEEEEECCEEEEEEEEECCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
PGAQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIQ
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHH
IVKEIGDDEILKAHTVDFISFSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQI
HHHHCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHCCCCCCCEEE
DPKGLRWTLNDLYSRYELPLFIVENGLGAYDKVEEDGKIHDDYRIEYLRKHIEQMKEAIA
CCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
CCCCEEEECCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHC
NGEDLD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]