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The map label for this gene is cysS [H]

Identifier: 116871622

GI number: 116871622

Start: 215435

End: 216850

Strand: Direct

Name: cysS [H]

Synonym: lwe0202

Alternate gene names: 116871622

Gene position: 215435-216850 (Clockwise)

Preceding gene: 116871621

Following gene: 116871623

Centisome position: 7.66

GC content: 37.64

Gene sequence:

>1416_bases
TTGTCGATACAAATATTTAATACGTTAACAAGAGAGAAGGAACCTTTTAAACCGCTCAAAGATGGCGAAGTAAAGATGTA
TGTTTGTGGGCCAACTGTTTATAATTACATTCATATCGGAAATGCGCGACCAATCATTGTTTTTGATACAGTTCGACGTT
ATTTCACGTATCGTGGTTATGATGTGAAATTTGTGTCTAATTTTACGGATGTGGATGATAAATTAATTCGTGCGGCAAAT
GAACTAAAATTGACGGTTCCAGAAGTGGCGGATCGCTTTATTGGTGCTTACTTTGATGATGTTGATCAGCTGAATGTGGC
AAAAGCTAGCGTGAATCCGCGTGTCACGGAAAATATGGATGAAATCATTCAATTGATAAGTACGCTAATCGAAAAAGGCT
ATGCATATGAGTCAGCTGGTGATGTCTATTTCCGAACAAAAAAATTCAAAGATTACGGTAAACTGTCTGGTCAAGAATTA
TCGGAACTACAACACGGGGCGCGAGTTGAATATAATGAACGCAAACAAGATGAGCTTGATTTCACACTATGGAAAGCTGC
TAAGCCAGATGAAATTTTCTGGGAGAGTCCGTTTGGTAATGGTCGGCCGGGTTGGCATATCGAATGTTCAGCGCTTGCGA
AAAAATATCTTGGAGATACCATTGATATTCATGCGGGAGGCCAAGACTTAGTTTTCCCTCACCATGAGGATGAAATTGCT
CAATCTGAAGCGGCGACTGGGAAAACTTTTGCAAATTACTGGATGCACAATGCTTTTTTAAATATTGATGGAGAAAAAAT
GTCCAAATCACTTGGGAATTTTATTACACTTCATGATGTGTTGAAAGATAACGATCCAAATGTGATTCGTTTCTTCATGC
TATCCGTACATTACCGTAAACCAATTACGTTAAATGATGCTATTTTAGAGGATGCAAAAAATGGTTTGGAACGACTAATG
ATTGCATACCAAAACATTGATCACCGTATTCAAACAGATGACGGTGAATATGTAGAGGAAGCACACGAAGATGAATGGTT
AGAACAACTGACAGAATTAAAACAAGCTTTTGAAGATGATATGGATGACGATTTTAATACAGCAAACGCGATTACTACTT
TCCATGAACTTGCAAAACGCGCTAATATTTACCTTGCAAAAGAGACAGTTTCTATTAATGTTTTGCGTGAATTTTTAAGT
ATGATGCGTTTGTTCGCTGAAGTATTAGGTTTGAAATTAGAAAATACGCAAACCGATTCGTTAGATGATAGTGAAGTAGA
AGCATTAATTGAAGAACGCCTGCAAGCGAGAAATGAACGTAATTTTGCAAGAGCGGATGAAATTCGAGATATTTTAAAAG
AAAAAAATATTATTTTGGAAGACACTGCGCAAGGCACGCGGTTTAGACGGGGGTAA

Upstream 100 bases:

>100_bases
AGTAGGCCACGCGGTTCCTAAAATGGACGAGCTAACATTACGAATTGCAGAATTAGAAAATATAGTAGAAAAACTTTTAA
AAGAAAAGGAGTAGGTCTGA

Downstream 100 bases:

>100_bases
ATAATGGCAGAAGTGAAGGAATACAAACAACTTAACGGCCTCGCGCTTGCTTATATGGGAGATGCGGTGTATGAGAAATT
TATCCGTGAATATTTACTTG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSIQIFNTLTREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAAN
ELKLTVPEVADRFIGAYFDDVDQLNVAKASVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQEL
SELQHGARVEYNERKQDELDFTLWKAAKPDEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLM
IAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLS
MMRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG

Sequences:

>Translated_471_residues
MSIQIFNTLTREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAAN
ELKLTVPEVADRFIGAYFDDVDQLNVAKASVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQEL
SELQHGARVEYNERKQDELDFTLWKAAKPDEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLM
IAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLS
MMRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
>Mature_470_residues
SIQIFNTLTREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAANE
LKLTVPEVADRFIGAYFDDVDQLNVAKASVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELS
ELQHGARVEYNERKQDELDFTLWKAAKPDEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIAQ
SEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLMI
AYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSM
MRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=480, Percent_Identity=37.7083333333333, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI303304987, Length=419, Percent_Identity=31.5035799522673, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI21269877, Length=419, Percent_Identity=31.5035799522673, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI62240992, Length=419, Percent_Identity=31.5035799522673, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI10835051, Length=419, Percent_Identity=31.5035799522673, Blast_Score=185, Evalue=8e-47,
Organism=Escherichia coli, GI1786737, Length=478, Percent_Identity=44.5606694560669, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17509795, Length=327, Percent_Identity=33.6391437308869, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17509797, Length=327, Percent_Identity=33.6391437308869, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=356, Percent_Identity=34.8314606741573, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=32.6732673267327, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI19922364, Length=324, Percent_Identity=32.0987654320988, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 54397; Mature: 54266

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIFNTLTREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGY
CEEEEEHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCC
DVKFVSNFTDVDDKLIRAANELKLTVPEVADRFIGAYFDDVDQLNVAKASVNPRVTENMD
EEEEECCCCCHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCHH
EIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELSELQHGARVEYNERKQDELD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC
FTLWKAAKPDEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
EEEECCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRK
HHHHCCCHHHHHHHHHHHEEECCHHHHHHHHCCEEEEHHHHCCCCCCEEEHEEHHHHCCC
PITLNDAILEDAKNGLERLMIAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDD
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSMMRLFAEVLGLKLENTQTDS
CCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
LDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SIQIFNTLTREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGY
EEEEEHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCC
DVKFVSNFTDVDDKLIRAANELKLTVPEVADRFIGAYFDDVDQLNVAKASVNPRVTENMD
EEEEECCCCCHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCHH
EIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELSELQHGARVEYNERKQDELD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC
FTLWKAAKPDEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
EEEECCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRK
HHHHCCCHHHHHHHHHHHEEECCHHHHHHHHCCEEEEHHHHCCCCCCEEEHEEHHHHCCC
PITLNDAILEDAKNGLERLMIAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDD
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSMMRLFAEVLGLKLENTQTDS
CCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
LDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]