Click here to switch to the map view.

The map label for this gene is pth [H]

Identifier: 116871595

GI number: 116871595

Start: 173541

End: 174095

Strand: Direct

Name: pth [H]

Synonym: lwe0175

Alternate gene names: 116871595

Gene position: 173541-174095 (Clockwise)

Preceding gene: 116871594

Following gene: 116871596

Centisome position: 6.17

GC content: 36.22

Gene sequence:

>555_bases
ATGAAATTAATCGCAGGACTCGGAAATCCGGGCAAAAAATACGAACGTACTCGGCATAATGTCGGTTTTATGGTAGTTGA
TGAATTGAGTTTTCGCCATCAGACCCCTTGGAAAAAATCAAAATTTAATGGTATGGTGAGTGAAATAAACGTTGGTGGCG
AAAAAATGATCTTGGTTAAACCGCTAACTTTTATGAACGCTTCTGGTGAATGTATTCGCCCTTTAATGGATTACTACAAT
ATCCAGGTAGAAGATGTTCTAATTGTATATGATGATTTAGACTTACCAGTAGGGAAAATAAGGTTACGACAAAAAGGTAG
CGCTGGCGGTCATAATGGAATGAAATCAATCATTCAGCACATTAAAACTCAAGAATTTAACCGTATCCGTGTAGGGGTCA
GTCGCCCTTTAAAAGGAGAAGTTATCAATTATGTTTTAGGTGATTTTCCTAAAGCAGAACAACCTGATATTATAGCAGCA
ATTCAAAAAAGTGCAGATGCTATCGAAGACTTTGCTCAAGTACCTTTCGTTGAAGTAATGAATAAATATAATTAA

Upstream 100 bases:

>100_bases
AACTTTCAACCAAGTGAAATCCATGTTATAATCAAAAAAACATTGAAACAAAAACCAGTTCATTCGTGAAGTGGTTTAGT
TCATGTAAGGAGAAGCTTTT

Downstream 100 bases:

>100_bases
TAAAATAGACTTAGATAAGGCGTGGCAATACTTTTGTCACGTATTTCGGTCTTTCTTCTAGCGTGTGATTTTATCTTGCA
TTGCGTAGAATGAATTTTCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMVSEINVGGEKMILVKPLTFMNASGECIRPLMDYYN
IQVEDVLIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHIKTQEFNRIRVGVSRPLKGEVINYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQVPFVEVMNKYN

Sequences:

>Translated_184_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMVSEINVGGEKMILVKPLTFMNASGECIRPLMDYYN
IQVEDVLIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHIKTQEFNRIRVGVSRPLKGEVINYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQVPFVEVMNKYN
>Mature_184_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMVSEINVGGEKMILVKPLTFMNASGECIRPLMDYYN
IQVEDVLIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHIKTQEFNRIRVGVSRPLKGEVINYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQVPFVEVMNKYN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=33.7209302325581, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1787455, Length=172, Percent_Identity=40.1162790697674, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 20751; Mature: 20751

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMVSEINVGGEKMILVK
CCEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCCHHEECCCCCEEEEEE
PLTFMNASGECIRPLMDYYNIQVEDVLIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQH
CHHHCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
IKTQEFNRIRVGVSRPLKGEVINYVLGDFPKAEQPDIIAAIQKSADAIEDFAQVPFVEVM
HHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
NKYN
HCCC
>Mature Secondary Structure
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMVSEINVGGEKMILVK
CCEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCCHHEECCCCCEEEEEE
PLTFMNASGECIRPLMDYYNIQVEDVLIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQH
CHHHCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
IKTQEFNRIRVGVSRPLKGEVINYVLGDFPKAEQPDIIAAIQKSADAIEDFAQVPFVEVM
HHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
NKYN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA