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The map label for this gene is ykfC [H]

Identifier: 116871556

GI number: 116871556

Start: 136377

End: 137255

Strand: Direct

Name: ykfC [H]

Synonym: lwe0136

Alternate gene names: 116871556

Gene position: 136377-137255 (Clockwise)

Preceding gene: 116871555

Following gene: 116871557

Centisome position: 4.85

GC content: 37.32

Gene sequence:

>879_bases
ATGAAAAAACTAGTTAGTCAAAGTAATGCGTTTCTTTGGAGAAAAAACGAGATGAATCCTGTATTAGCCAATATTTTAGA
GGCCAGAAAAAATGATGATTTGGTTCAAGTTAGCCAACAAGACGCTATGAAATTATATGATGATAGCCTTGTTGACTCCG
ATTTACTATATAACGATTCAGTGATAGTTGATAAAGTAGAAGGCGACTGGGCAAAAGTAGTTGTTCCCTCCCAGCATAGC
TTTCTGGATGAACGTGGTTATCCAGGGTGGATGTTGTTAAAAGACCTCGCAGACACAGATGAAATAGAAGATACCAATGA
GAAAATTGCTGTAATTATACCAAAAACTCAAATCACATATGAAAACGGTGATAAAAGAACTGTGTCATTTGGAACACTTT
TTACAAAAATTTCTGAAGCGGATGGACTTTATGAGATTGTAACACCCCATGGTCCAGCCACGATTTTAAAAAGCCAAGTA
GAATTACAGCATAAAACTCGCGATAATATTGGTGTGGATGTTGTACAAATGGCGATGCAATTCCTTGATTTACCATACGT
TTGGGCAGGAATTAGTAGTGCTGGTTTTGATTGTTCCGGATTTGCTTTCACACTTTACCGAACTTGTGGAAAATATATTG
GGCGTGATGCCACTGAACAATCTTTCGCAGGTGAAAAAATTGATTATAAGAATGCAGAGCCAGGTGATTTGCTATTTTTC
GCATATGAAGAAGGTAAAGGAGAAGTTCATCATGTTGGCATTTACATCGGAAATGACGAAATGATTCATTCACAGACTCC
AGGTTCCAAAGTGATTTTAACTAAAATTGCTGGCAGTAAATACGAACCAGAACTTTGTGTTACTTCAAGGCATCGCTAA

Upstream 100 bases:

>100_bases
AATTTAACCGACATCGGCCTTGAAATTGGTCGTGGGCAAGTACTTTTAACAGAAGAAGATGGGATTGGAATTCCTGCTTA
TTAAAGAAAGAAGGGTTAAA

Downstream 100 bases:

>100_bases
AGGGGTGGGTGAAACTGACCAATTATCAAAAAATTAACCAAGATTTCAACACGATTGATACAAAAGCGCTGTATATTACA
GATGAAAACAAAGTCCTTTT

Product: NlpC/P60 family protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKKLVSQSNAFLWRKNEMNPVLANILEARKNDDLVQVSQQDAMKLYDDSLVDSDLLYNDSVIVDKVEGDWAKVVVPSQHS
FLDERGYPGWMLLKDLADTDEIEDTNEKIAVIIPKTQITYENGDKRTVSFGTLFTKISEADGLYEIVTPHGPATILKSQV
ELQHKTRDNIGVDVVQMAMQFLDLPYVWAGISSAGFDCSGFAFTLYRTCGKYIGRDATEQSFAGEKIDYKNAEPGDLLFF
AYEEGKGEVHHVGIYIGNDEMIHSQTPGSKVILTKIAGSKYEPELCVTSRHR

Sequences:

>Translated_292_residues
MKKLVSQSNAFLWRKNEMNPVLANILEARKNDDLVQVSQQDAMKLYDDSLVDSDLLYNDSVIVDKVEGDWAKVVVPSQHS
FLDERGYPGWMLLKDLADTDEIEDTNEKIAVIIPKTQITYENGDKRTVSFGTLFTKISEADGLYEIVTPHGPATILKSQV
ELQHKTRDNIGVDVVQMAMQFLDLPYVWAGISSAGFDCSGFAFTLYRTCGKYIGRDATEQSFAGEKIDYKNAEPGDLLFF
AYEEGKGEVHHVGIYIGNDEMIHSQTPGSKVILTKIAGSKYEPELCVTSRHR
>Mature_292_residues
MKKLVSQSNAFLWRKNEMNPVLANILEARKNDDLVQVSQQDAMKLYDDSLVDSDLLYNDSVIVDKVEGDWAKVVVPSQHS
FLDERGYPGWMLLKDLADTDEIEDTNEKIAVIIPKTQITYENGDKRTVSFGTLFTKISEADGLYEIVTPHGPATILKSQV
ELQHKTRDNIGVDVVQMAMQFLDLPYVWAGISSAGFDCSGFAFTLYRTCGKYIGRDATEQSFAGEKIDYKNAEPGDLLFF
AYEEGKGEVHHVGIYIGNDEMIHSQTPGSKVILTKIAGSKYEPELCVTSRHR

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=106, Percent_Identity=36.7924528301887, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 32744; Mature: 32744

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVSQSNAFLWRKNEMNPVLANILEARKNDDLVQVSQQDAMKLYDDSLVDSDLLYNDS
CCCHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCEECCC
VIVDKVEGDWAKVVVPSQHSFLDERGYPGWMLLKDLADTDEIEDTNEKIAVIIPKTQITY
EEEECCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCEEEEE
ENGDKRTVSFGTLFTKISEADGLYEIVTPHGPATILKSQVELQHKTRDNIGVDVVQMAMQ
ECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FLDLPYVWAGISSAGFDCSGFAFTLYRTCGKYIGRDATEQSFAGEKIDYKNAEPGDLLFF
HHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCEEEE
AYEEGKGEVHHVGIYIGNDEMIHSQTPGSKVILTKIAGSKYEPELCVTSRHR
EEECCCCCEEEEEEEECCCCEEECCCCCCEEEEEEECCCCCCCCEEECCCCC
>Mature Secondary Structure
MKKLVSQSNAFLWRKNEMNPVLANILEARKNDDLVQVSQQDAMKLYDDSLVDSDLLYNDS
CCCHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCEECCC
VIVDKVEGDWAKVVVPSQHSFLDERGYPGWMLLKDLADTDEIEDTNEKIAVIIPKTQITY
EEEECCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCEEEEE
ENGDKRTVSFGTLFTKISEADGLYEIVTPHGPATILKSQVELQHKTRDNIGVDVVQMAMQ
ECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FLDLPYVWAGISSAGFDCSGFAFTLYRTCGKYIGRDATEQSFAGEKIDYKNAEPGDLLFF
HHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCEEEE
AYEEGKGEVHHVGIYIGNDEMIHSQTPGSKVILTKIAGSKYEPELCVTSRHR
EEECCCCCEEEEEEEECCCCEEECCCCCCEEEEEEECCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]