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The map label for this gene is ykfB [H]

Identifier: 116871555

GI number: 116871555

Start: 135284

End: 136360

Strand: Direct

Name: ykfB [H]

Synonym: lwe0135

Alternate gene names: 116871555

Gene position: 135284-136360 (Clockwise)

Preceding gene: 116871554

Following gene: 116871556

Centisome position: 4.81

GC content: 37.51

Gene sequence:

>1077_bases
TTGAAAATCACAAAAATTAAAACCCATAGCTTGCCAATTCAACTAAAAGCGACTTTTAAAACAGCACTTAGAGTAGTTAA
TCATGTGAATGTTGTGCGAGTGTATATTCATTTTGATAACGGGATTGTTGGTATTGGAGAAGCTGCCCCAACTCATGTAA
TTACAGGGGATACAGAAGTGAGTATTACAAGTGCGATAAATGATATTTTTAGCCCTTTTTTAATTGGGCGTGAGCTAGAT
GAAGAACTTACCATTTTAGATGAGATGAAATCGCTTTTAGTTCATAATTCAAGTCCAAAAGCCGCCATTGATATTGCTTT
ACACGATGCTCTGGCAAAAGCCCACTCAAAACCATTGTATGAATTTCTTGGTGGGGGAACTACAAAACTAGAAACAGATT
ATACTATTAGCATCGGGACACCAGAAGAAATGGTTCGAGACGCAGAAATCAAGGTATCAGAAGGCTTTCAGTCGCTTAAA
GTTAAACTTGGTCTTGATCCTGTAGAAATAGAAGTAGCAAAAATCAGACAAATGAACGAAGCATTAGGAGGTAAAATTCC
TTTTAGAATCGATGCAAATCAAGGGTGGACCGCAGATGAAGCGATTCAAATTCTAAATGAATGGCATGACATTCCGATTG
ATTTTATTGAGCAACCGGTTAAAAGTTGGGATTTCTCTGGAATGACAAAAGTGACTGCAAATACCAATATCCCTATCATG
GCAGATGAGAGTTTATTTGGAATTCACGATGCTAAGCGACTTTTAGATGAAAAATGCTGTAACTTACTTAATATTAAATT
GATGAAAAGCTCTGGAATTAGAGAAGCGCGTGCGATACATGAGTTAGCAAGTAGCCATAATGTTGACTGTATGATTGGGA
CGATGATTGAAGGATATGCAAGTTTATCAGCAGCAGCTCATTTCGCAGCGGCATCTAAGCAAGTGAAGTTTTATGATTTA
GATGTACCGTTTATGTGGAATCTACATGGCAAAAATTTAACCGACATCGGCCTTGAAATTGGTCGTGGGCAAGTACTTTT
AACAGAAGAAGATGGGATTGGAATTCCTGCTTATTAA

Upstream 100 bases:

>100_bases
GATGGGAGATATAATCCCTAGCCAATCTTGCCAAATGTGTACTGTTTTGCTATTGTTGTAAGAATAGTTTGACATTTTTA
CGTTAAAGGAGTGAAATTGC

Downstream 100 bases:

>100_bases
AGAAAGAAGGGTTAAAATGAAAAAACTAGTTAGTCAAAGTAATGCGTTTCTTTGGAGAAAAAACGAGATGAATCCTGTAT
TAGCCAATATTTTAGAGGCC

Product: mandelate racemase/muconate lactonizing family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKITKIKTHSLPIQLKATFKTALRVVNHVNVVRVYIHFDNGIVGIGEAAPTHVITGDTEVSITSAINDIFSPFLIGRELD
EELTILDEMKSLLVHNSSPKAAIDIALHDALAKAHSKPLYEFLGGGTTKLETDYTISIGTPEEMVRDAEIKVSEGFQSLK
VKLGLDPVEIEVAKIRQMNEALGGKIPFRIDANQGWTADEAIQILNEWHDIPIDFIEQPVKSWDFSGMTKVTANTNIPIM
ADESLFGIHDAKRLLDEKCCNLLNIKLMKSSGIREARAIHELASSHNVDCMIGTMIEGYASLSAAAHFAAASKQVKFYDL
DVPFMWNLHGKNLTDIGLEIGRGQVLLTEEDGIGIPAY

Sequences:

>Translated_358_residues
MKITKIKTHSLPIQLKATFKTALRVVNHVNVVRVYIHFDNGIVGIGEAAPTHVITGDTEVSITSAINDIFSPFLIGRELD
EELTILDEMKSLLVHNSSPKAAIDIALHDALAKAHSKPLYEFLGGGTTKLETDYTISIGTPEEMVRDAEIKVSEGFQSLK
VKLGLDPVEIEVAKIRQMNEALGGKIPFRIDANQGWTADEAIQILNEWHDIPIDFIEQPVKSWDFSGMTKVTANTNIPIM
ADESLFGIHDAKRLLDEKCCNLLNIKLMKSSGIREARAIHELASSHNVDCMIGTMIEGYASLSAAAHFAAASKQVKFYDL
DVPFMWNLHGKNLTDIGLEIGRGQVLLTEEDGIGIPAY
>Mature_358_residues
MKITKIKTHSLPIQLKATFKTALRVVNHVNVVRVYIHFDNGIVGIGEAAPTHVITGDTEVSITSAINDIFSPFLIGRELD
EELTILDEMKSLLVHNSSPKAAIDIALHDALAKAHSKPLYEFLGGGTTKLETDYTISIGTPEEMVRDAEIKVSEGFQSLK
VKLGLDPVEIEVAKIRQMNEALGGKIPFRIDANQGWTADEAIQILNEWHDIPIDFIEQPVKSWDFSGMTKVTANTNIPIM
ADESLFGIHDAKRLLDEKCCNLLNIKLMKSSGIREARAIHELASSHNVDCMIGTMIEGYASLSAAAHFAAASKQVKFYDL
DVPFMWNLHGKNLTDIGLEIGRGQVLLTEEDGIGIPAY

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=287, Percent_Identity=27.8745644599303, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994953, Length=325, Percent_Identity=26.4615384615385, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39399; Mature: 39399

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKIKTHSLPIQLKATFKTALRVVNHVNVVRVYIHFDNGIVGIGEAAPTHVITGDTEV
CCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCCCEEEECCCCE
SITSAINDIFSPFLIGRELDEELTILDEMKSLLVHNSSPKAAIDIALHDALAKAHSKPLY
EHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHCCCHH
EFLGGGTTKLETDYTISIGTPEEMVRDAEIKVSEGFQSLKVKLGLDPVEIEVAKIRQMNE
HHHCCCCEEEECCEEEEECCHHHHHHCCCEEECCCCEEEEEEECCCCCEEHHHHHHHHHH
ALGGKIPFRIDANQGWTADEAIQILNEWHDIPIDFIEQPVKSWDFSGMTKVTANTNIPIM
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
ADESLFGIHDAKRLLDEKCCNLLNIKLMKSSGIREARAIHELASSHNVDCMIGTMIEGYA
ECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
SLSAAAHFAAASKQVKFYDLDVPFMWNLHGKNLTDIGLEIGRGQVLLTEEDGIGIPAY
HHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHCEEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKITKIKTHSLPIQLKATFKTALRVVNHVNVVRVYIHFDNGIVGIGEAAPTHVITGDTEV
CCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCCCEEEECCCCE
SITSAINDIFSPFLIGRELDEELTILDEMKSLLVHNSSPKAAIDIALHDALAKAHSKPLY
EHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHCCCHH
EFLGGGTTKLETDYTISIGTPEEMVRDAEIKVSEGFQSLKVKLGLDPVEIEVAKIRQMNE
HHHCCCCEEEECCEEEEECCHHHHHHCCCEEECCCCEEEEEEECCCCCEEHHHHHHHHHH
ALGGKIPFRIDANQGWTADEAIQILNEWHDIPIDFIEQPVKSWDFSGMTKVTANTNIPIM
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
ADESLFGIHDAKRLLDEKCCNLLNIKLMKSSGIREARAIHELASSHNVDCMIGTMIEGYA
ECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
SLSAAAHFAAASKQVKFYDLDVPFMWNLHGKNLTDIGLEIGRGQVLLTEEDGIGIPAY
HHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHCEEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]