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The map label for this gene is bglH [H]

Identifier: 116871440

GI number: 116871440

Start: 20416

End: 21849

Strand: Direct

Name: bglH [H]

Synonym: lwe0018

Alternate gene names: 116871440

Gene position: 20416-21849 (Clockwise)

Preceding gene: 116871439

Following gene: 116871442

Centisome position: 0.73

GC content: 39.47

Gene sequence:

>1434_bases
ATGACAGAATCAAAATTTCCTAAAGGCTTTTTATGGGGCGGAGCAGTTGCTGCAAACCAATGTGAAGGCGCTTATCTTGA
AGACGGTAAAGGACTTTCACTAGTAGATATAGTACCAACAGTAGAGGACGGACGTTGGGACGCACTTTTCAATCCAACGA
AAGCGCTTTCCACTGATTATGGTTTTTACCCAAGTCATGAATCAATTGATTTTTATCATCGTTATAAAGAAGACATTAAA
TTAATGGCGGATATGGGATTCAAATGTTTCCGTATGTCCATCAGCTGGCCACGTATTTTCCCAAATGGTGACGAAACTAC
ACCAAATGAAAAAGGATTAGCATTTTATGACGCAGTTTTTGATGAGTGTCATAAATATGGTATCGAGCCAGTAGTTACAA
TCAATCATTTCGATACTCCACTAGAAGTTTTCAAAAAATATGGTGGTTGGAAAAACCGCAAATGTATCGACTTTTACTTG
AATTTCTGTGAAGCTATTTTCACACGTTATAAAGATAAAGTAAAATATTGGATGACATTTAACGAAATTAACATGATTCT
TCATATTCCATTCTTTGGTGGCGGCATGGACGTTACGAATGAAGAAAACCCAGAAGAAGTCAAATACCAAGCGGCACATC
ACCAATTAGTTGCATCTGCTTTAGCAACAAAACTTGGTCATGAAATCAACCCGGAAAACCAAATAGGTTGTATGCTTGCA
GCTGGGAACACATACCCAATGACATGTAATCCACAAGATGTTTGGAAATCCATTCAAGCGGACCGTGAAGGCTATTTCTT
CATTGACGTTCAAGCGCGTGGTTACTATCCAAGCTACACTAAACGCTTCTTCAAAGAACACAACATTAACATCAAAATGG
AAGATGGCGACTTAGACGCATTACGCGATCACACAGTTGATTATGTGGCATTCAGTTACTATTCTTCTCGTCTAACAAGC
GCAGATCCAGAGAAAAGCAAAGAAACAGAAGGCAATGTTTTTGCAACACTAAAAAACCCATATCTAAAAGCAAGCGAATG
GGGTTGGCAAATTGATCCACTAGGTCTTCGTATCACTATGAATACAATTTATGACCGTTATCAAAAACCACTTTTCATTG
TAGAAAATGGATTAGGTGCAGTAGATACAGTGGAAGAAGATGGCGCTATTAATGATGATTACAGAATCGATTACATGCGT
GAACACGTTCGCGAAATGGGTGAAGCAATCGAGGACGGCGTGGAACTTCTTGGTTACACACCATGGGGCTGCATCGACCT
TGTCAGCGCTGGCTCTGGTGAAATGAAAAAACGTTATGGCTTCATCTACGTTGACCGCGACAACAAAGGCAACGGAACAC
TAAACCGCTCGAAGAAAAAATCATTCGACTGGTACAAAAAAGTAATTGAAACAAACGGTAAAGATATCGATTAA

Upstream 100 bases:

>100_bases
AGACGAAATAAAAAATTTCTAAAATTATTGCTTGCTATGTAATGCATTACATATTCTATAGTTAAGAAGTAAATTATATT
CGTGAGAGGAGTTACACAAA

Downstream 100 bases:

>100_bases
TAACTAACAAACCCCCACTTTCGGCGATGAAGGTGGGGGTTTTAAGTAATATTATTTATGGGAAGTTTCTTCTTTTTCGA
TTTCAGAAACAGACTTATCT

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTESKFPKGFLWGGAVAANQCEGAYLEDGKGLSLVDIVPTVEDGRWDALFNPTKALSTDYGFYPSHESIDFYHRYKEDIK
LMADMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYL
NFCEAIFTRYKDKVKYWMTFNEINMILHIPFFGGGMDVTNEENPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
AGNTYPMTCNPQDVWKSIQADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTS
ADPEKSKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVEEDGAINDDYRIDYMR
EHVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID

Sequences:

>Translated_477_residues
MTESKFPKGFLWGGAVAANQCEGAYLEDGKGLSLVDIVPTVEDGRWDALFNPTKALSTDYGFYPSHESIDFYHRYKEDIK
LMADMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYL
NFCEAIFTRYKDKVKYWMTFNEINMILHIPFFGGGMDVTNEENPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
AGNTYPMTCNPQDVWKSIQADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTS
ADPEKSKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVEEDGAINDDYRIDYMR
EHVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
>Mature_476_residues
TESKFPKGFLWGGAVAANQCEGAYLEDGKGLSLVDIVPTVEDGRWDALFNPTKALSTDYGFYPSHESIDFYHRYKEDIKL
MADMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLN
FCEAIFTRYKDKVKYWMTFNEINMILHIPFFGGGMDVTNEENPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLAA
GNTYPMTCNPQDVWKSIQADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTSA
DPEKSKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVEEDGAINDDYRIDYMRE
HVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=495, Percent_Identity=27.2727272727273, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=27.4549098196393, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI28376633, Length=487, Percent_Identity=28.5420944558522, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI13273313, Length=486, Percent_Identity=26.7489711934156, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI24497614, Length=491, Percent_Identity=27.6985743380855, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=59.915611814346, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI2367270, Length=471, Percent_Identity=54.140127388535, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=477, Percent_Identity=55.3459119496855, Blast_Score=537, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17552856, Length=491, Percent_Identity=28.9205702647658, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17539390, Length=494, Percent_Identity=28.1376518218623, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21356577, Length=498, Percent_Identity=25.1004016064257, Blast_Score=145, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54835; Mature: 54704

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESKFPKGFLWGGAVAANQCEGAYLEDGKGLSLVDIVPTVEDGRWDALFNPTKALSTDY
CCCCCCCCCEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCC
GFYPSHESIDFYHRYKEDIKLMADMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVF
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHH
DECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTF
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
NEINMILHIPFFGGGMDVTNEENPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
EEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
AGNTYPMTCNPQDVWKSIQADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDA
ECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHH
LRDHTVDYVAFSYYSSRLTSADPEKSKETEGNVFATLKNPYLKASEWGWQIDPLGLRITM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCEECCCEEEEEH
NTIYDRYQKPLFIVENGLGAVDTVEEDGAINDDYRIDYMREHVREMGEAIEDGVELLGYT
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
PWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
CCCHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TESKFPKGFLWGGAVAANQCEGAYLEDGKGLSLVDIVPTVEDGRWDALFNPTKALSTDY
CCCCCCCCEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCC
GFYPSHESIDFYHRYKEDIKLMADMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVF
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHH
DECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTF
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
NEINMILHIPFFGGGMDVTNEENPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
EEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
AGNTYPMTCNPQDVWKSIQADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDA
ECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHH
LRDHTVDYVAFSYYSSRLTSADPEKSKETEGNVFATLKNPYLKASEWGWQIDPLGLRITM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCEECCCEEEEEH
NTIYDRYQKPLFIVENGLGAVDTVEEDGAINDDYRIDYMREHVREMGEAIEDGVELLGYT
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
PWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
CCCHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]