Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 116691170

GI number: 116691170

Start: 3472051

End: 3473142

Strand: Reverse

Name: ada [H]

Synonym: Bcen2424_3152

Alternate gene names: 116691170

Gene position: 3473142-3472051 (Counterclockwise)

Preceding gene: 116691172

Following gene: 116691169

Centisome position: 99.69

GC content: 71.52

Gene sequence:

>1092_bases
ATGAAAACCGCCTATCCGACCGACGATGCCCGCTGGGGCGCCGTGACCGAGCGCGATCCGCATGCGGACGGCGCGTTCTT
CTATGCCGTCAGCACGACCGGCGTGTTCTGCCGCCCGACCTGCGCGTCGCGGCAGCCGCGCCGCGAGAACGTGTCCTTTT
TCGCCGATCCGGCTGCCGCACGCGCGGCCGGCTTCCGGCCCTGCAAGCGTTGCCAGCCGGAAGGGCTGCCGCGCGAGCTG
GAAATCGTCAATCGCGCATGCGCGGTGCTCGACGCGCACGCCGAGCGGCTCACGCTGCAGCAGTTGAGCGACGCCGTGCA
CGTGAGCCCGTTCCACCTGCAGCGGCTCTTCAAACGTGTGGTCGGCGTGTCGCCGCGGCAGTACCAGGCCGCGCAGCGCG
GCGCCGCGTTGCGGCAGGCGCTGCAAAGCGGGCAACCCGTCACGCAGGCAGCCGTCGACGCGGGGTTCAACTCGCCGTCG
CGGCTCTATGCGTCGGTGCCGCGCGAGTTGGGGATGGCGCCGTCCGCGTTCCGCCGCCAGGGCGCGGGCCTGCGGATCGA
CTATGCGACCGCGTCGACGTCGCTCGGCACGGTGCTCGTCGCCGCCACCGAACAGGGCATCTGCCGGATCGCGTTCGGCG
ACGACGCGGTGTCGCTCGTCGGCGAGCTGAACGACACGTTCGCACGCGCCGAACTGGTCGAAGCGTCGGCCCGGCTCGCG
CCGTTCGTCACGCAGATCCGCGCGTACCTCGACGGCACGCGGCAGGCGTTCGACCTGCCGCTCGACATCGCGCCGACCGC
GTTCCAGCAGCGCGTGTGGGAAGCGCTGACGCATATCCCGTACGGTGAAACGCGCAGCTATTCGGAGATCGCCGAGGCGC
TGGGTGCGCCGCGCGCGGTGCGGGCGGTCGCGTCCGCGTGCGCGTCGAATCCGGTCGCGCTCGCGATTCCGTGCCACCGC
GTCGTGCAGAAGGGCGGCGCGCTCGCCGGCTATCGCTGGGGCGTGCGCCGCAAGGCGACGCTGCTCGCGGCCGAGGCGCG
CCATGTTCACAACGATGAAGCCGAAGCCGAGACGGAGGACAGTGCGGTTTGA

Upstream 100 bases:

>100_bases
CCGGCTAGCCGGCACGAATCACAGAGCAAGATTCAGGGCGCGACGGCATGTGCCGATGGCTAGAGTAGGCGCCATCTACA
CTAGAGAGTGCGAGGTTCAG

Downstream 100 bases:

>100_bases
GCATCGACACGACAACGATTACCTCCCTCGCGAACGGCGGCCCTGTGCCGCCGTCCGCGCAGATGGAGCTGCGCTACAAG
GCGCCGTACGACTGGGCGCG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFADPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDDAVSLVGELNDTFARAELVEASARLA
PFVTQIRAYLDGTRQAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGAPRAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLAAEARHVHNDEAEAETEDSAV

Sequences:

>Translated_363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFADPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDDAVSLVGELNDTFARAELVEASARLA
PFVTQIRAYLDGTRQAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGAPRAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLAAEARHVHNDEAEAETEDSAV
>Mature_363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFADPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDDAVSLVGELNDTFARAELVEASARLA
PFVTQIRAYLDGTRQAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGAPRAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLAAEARHVHNDEAEAETEDSAV

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=30.7228915662651, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=49.2753623188406, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=35.5421686746988, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=125, Percent_Identity=43.2, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=125, Percent_Identity=43.2, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=156, Percent_Identity=29.4871794871795, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17137554, Length=131, Percent_Identity=41.9847328244275, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39156; Mature: 39156

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFADPAAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCEEECCHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPSRLYASVPRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHC
GAGLRIDYATASTSLGTVLVAATEQGICRIAFGDDAVSLVGELNDTFARAELVEASARLA
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
PFVTQIRAYLDGTRQAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGAPRAV
HHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALAGYRWGVRRKATLLAAEARHVHNDEAEAETED
HHHHHHHCCCCEEEEECHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCHHCCCCC
SAV
CCC
>Mature Secondary Structure
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFADPAAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCEEECCHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPSRLYASVPRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHC
GAGLRIDYATASTSLGTVLVAATEQGICRIAFGDDAVSLVGELNDTFARAELVEASARLA
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
PFVTQIRAYLDGTRQAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGAPRAV
HHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALAGYRWGVRRKATLLAAEARHVHNDEAEAETED
HHHHHHHCCCCEEEEECHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCHHCCCCC
SAV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]