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The map label for this gene is exoA [H]

Identifier: 116691123

GI number: 116691123

Start: 3418597

End: 3419370

Strand: Reverse

Name: exoA [H]

Synonym: Bcen2424_3105

Alternate gene names: 116691123

Gene position: 3419370-3418597 (Counterclockwise)

Preceding gene: 116691124

Following gene: 116691121

Centisome position: 98.15

GC content: 62.14

Gene sequence:

>774_bases
ATGATGCGAGTGATTACCGCCAACCTGAATGGCATCCGCTCCGCCGCGAAGAAGGGCTTCTTCGACTGGCTCGGCGAACA
GAACGCCGATTGCGTATGCGTGCAGGAAATCAAGGTGTCGGCCGACGACCTGCCGGCCGAATTCGTCGAGCCGCACGGCT
TCAAGAGCTATTTCCACCACGCCGAGAAGAAGGGCTACAGCGGCGCGGGCGTGTACAGCCGCCGCGAGCCCGATGACGTG
ATCATCGGTTTCGGCAGCAGCGAGTTCGATCCCGAAGGGCGCTACGTCGAGGCGCGCTACGGCAAGCTGTCGGTCGTGTC
GGTGTACGTGCCGTCCGGTTCGAGCGGCGAGGAGCGCCAGCAGGCGAAGTACCGCTTCATGGCCGAATTCATGCCGCACC
TCGCGGCGCTGAAGAAGAAGCGCGAGGTGATCCTGTGCGGCGACGTGAACATCGTCCACAAGGAAATCGACATCAAGAAC
TGGAAGAGCAACCAGAAGAACTCGGGCTGCCTGCCGGAAGAACGCGAGTGGCTCACGAAGCTGTTCGACGACGTCGGCTA
CGTCGACGTGTTCCGCACGCTCGATCAGCGCCCCGAGCAGTACACGTGGTGGAGCAACCGCGGCCAGGCGTACGCGAAGA
ACGTCGGGTGGCGGATCGATTACCAGATCGCGACCCCGGGCGTGGCCGGGACCGCGAAAAGCACATCGATCTTCAAGGAC
ATCAAGTTCAGCGATCACGCGCCGCTGACGATCGACTACGACTACCAGAAGTGA

Upstream 100 bases:

>100_bases
ACATGAAGCTGGAATTCCCGCCGCCGAAGCCCGAGCTCGAAGGCGTGAAGATCCGTTAAGCTGCCCCCCTGTAAAAACAT
CGAACTGAACGGAACCCGAC

Downstream 100 bases:

>100_bases
TGCGCTGGGTGGCGCCTGGCGCGTAGCCGGATGACGCGATGACGCAAAACGGGCCGCATGTCGAACATGCGGCCCGTTTC
GTTTGGCGCGGCTTACTGCT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MMRVITANLNGIRSAAKKGFFDWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDPEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMAEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYQK

Sequences:

>Translated_257_residues
MMRVITANLNGIRSAAKKGFFDWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDPEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMAEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYQK
>Mature_257_residues
MMRVITANLNGIRSAAKKGFFDWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDPEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMAEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYQK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=36.3636363636364, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=36.3636363636364, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=36.3636363636364, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=29.6992481203008, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=30.5019305019305, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=33.8645418326693, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=33.8645418326693, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29276; Mature: 29276

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRVITANLNGIRSAAKKGFFDWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHH
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCCCCHHHHHHH
AEKKGYSGAGVYSRREPDDVIIGFGSSEFDPEGRYVEARYGKLSVVSVYVPSGSSGEERQ
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMAEFMPHLAALKKKREVILCGDVNIVHKEIDIKNWKSNQKNSGCLPEEREWLTK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDDVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCCCCCCCCHHHHHH
IKFSDHAPLTIDYDYQK
CCCCCCCCEEEECCCCC
>Mature Secondary Structure
MMRVITANLNGIRSAAKKGFFDWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHH
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCCCCHHHHHHH
AEKKGYSGAGVYSRREPDDVIIGFGSSEFDPEGRYVEARYGKLSVVSVYVPSGSSGEERQ
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMAEFMPHLAALKKKREVILCGDVNIVHKEIDIKNWKSNQKNSGCLPEEREWLTK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDDVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCCCCCCCCHHHHHH
IKFSDHAPLTIDYDYQK
CCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]