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The map label for this gene is metK [H]

Identifier: 116691099

GI number: 116691099

Start: 3395151

End: 3396338

Strand: Reverse

Name: metK [H]

Synonym: Bcen2424_3081

Alternate gene names: 116691099

Gene position: 3396338-3395151 (Counterclockwise)

Preceding gene: 116691101

Following gene: 116691097

Centisome position: 97.49

GC content: 65.07

Gene sequence:

>1188_bases
GTGGCAAACGATTATCTCTTCACGTCCGAATCCGTCTCCGAAGGCCATCCGGACAAAGTCGCGGACCAAATCTCGGACGC
GATTCTCGACGCCATCCTCGAGCAGGACAAATACTCCCGCGTTGCGGCGGAAACGCTGTGCAACACGGGTCTCGTCGTGC
TGGCCGGTGAAATCACCACGACGGCCAACATCGATTACATCCAGATCGCGCGCGATACGATCAAGCGCATCGGCTACGAC
AACACCGACTACGGCATCGACTACAAGGGTTGCGCGGTGCTCGTCGCGTACGACAAGCAGTCGCCGGACATCGCGCAGGG
CGTCGATCGTGCACACGACGACAACCTCGACCAAGGCGCGGGCGACCAGGGCCTGATGTTCGGTTATGCGTGCGACGAAA
CGCCGGAACTGATGCCGCTGCCGATCTACCTGTCGCACCGCCTCGTCGAGCGTCAGGCGAGCCTGCGCCGCGACGGTCGC
CTGCAATGGCTGCGTCCGGACGCGAAGTCGCAGGTCACCGTCCGCTACGTCGACGGCAAGCCCGATTCGATCGACACCGT
CGTGCTGTCGACGCAGCACGCACCGGACATCGAGCTGCCCGCGCTGCGCGAAGCCGTGATCGAGGAAATCATCAAGCCGA
CGCTGCCGGCCGAGCTGATCAAGGGCGACATCAAGTTCCTCGTGAACCCGACCGGCCGGTTCGTGATCGGCGGCCCGCAG
GGCGATTGCGGCCTGACCGGCCGCAAGATCATCGTCGATACCTACGGCGGTGCCGCACCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGATCCGTCGAAGGTCGACCGTTCGGCTGCGTATGCCGGCCGTTACGTCGCGAAGAACATCGTCGCCGCCGGCC
TCGCGTCGCGCGCGCTGATCCAGGTGTCGTACGCGATCGGCGTGGCCGAGCCGACCTCGGTGATGGTCAACACGTTCGGC
ACAGGCCGCGTGTCGGATGCGGTGATCACGAAGCTCGTGCGCGAGCATTTCGACCTGCGTCCGAAGGGCATCATCAAGAT
GCTCGACCTGCTGCGCCCGATTTACGAGAAGACCGCCGCTTACGGCCACTTCGGCCGCGAAGAGCCGGAATTCTCGTGGG
AAGCAACCGACAAGGCGCTCGCGCTGGCCGAAGCGGCCGGCGTGGAGCCGACGGCACGCGTCGCGTAA

Upstream 100 bases:

>100_bases
CACGCTGCCCGGTCCTGGTAATCCGCTAAAGCGTCGCCGCTTCAGGCCCAACGAAGCTGGCTACGTGAAACCAACCGTAA
CCACACAGGAGCCTGAAAAA

Downstream 100 bases:

>100_bases
GCGTCGTTCGCCTGAAATGAAAAACCCCGGCCTAGCCGGGGTTTTTTATGGGTGTCGATCGTCGCGGCAACGGCGGCGAT
CGGTGCGGGCCATGCCCGCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGR
LQWLRPDAKSQVTVRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA

Sequences:

>Translated_395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGR
LQWLRPDAKSQVTVRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
>Mature_394_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDN
TDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRL
QWLRPDAKSQVTVRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGT
GRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=58.2010582010582, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=56.3492063492063, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=69.8701298701299, Blast_Score=536, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.6842105263158, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.5106382978723, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=58.7765957446808, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=55.7840616966581, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=53.1914893617021, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=57.2289156626506, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.6219512195122, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.8073089700997, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=57.1428571428571, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=58.8390501319261, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=58.994708994709, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=54.5454545454545, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=54.5454545454545, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52.2666666666667, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=54.6218487394958, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42709; Mature: 42578

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLQWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
PDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLQWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
PDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA