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The map label for this gene is alkJ [H]

Identifier: 116691088

GI number: 116691088

Start: 3384420

End: 3386060

Strand: Reverse

Name: alkJ [H]

Synonym: Bcen2424_3070

Alternate gene names: 116691088

Gene position: 3386060-3384420 (Counterclockwise)

Preceding gene: 116691091

Following gene: 116691081

Centisome position: 97.19

GC content: 69.47

Gene sequence:

>1641_bases
ATGCAATACGACTACATCATCGTCGGCGGCGGTTCGGGCGGCTCGAGCCTGGCCGGCCGTCTCGCCGATGCCTGCCCCGA
TGCGACGATCGCGCTGATCGAGGCCGGCTCGCATACGGAGCGCAACCTGCTCGTCAACATGCCGGTCGGCATCGCGGCGC
TGGTGCCGTACAAGCTCGGCACCAACTACGGCTACGAGACGGTGCCGCAGCCGGGCCTGGGCGGCCGGCGCGGTTACCAG
CCGCGCGGGCGCGGGATGGGCGGATCGAGCGCGATCAACGCGATGATCTATACGCGCGGCCACCCGGGCGATTACGACGA
GTGGGCGCAGCTCGGCGCGACGGGCTGGGGCTGGCAGGAGGTGCTGCCGTATTTCCGCCGCGCGGAAGGCAACGAGCGCG
GCGCGGATGAATGGCACGGCGCGGACGGCCCGTTGACCGTTTCCGATCTGCGCTTTCGCAATCCGTTCTCCGAACGATTC
ATCCAGGCCGCGCATGCCGCCGGCTATCCGCTGAACGACGACTTCAACGGCGCGACGCAGGAAGGCGTCGGTTTCTATCA
GGTCACGCATCGCGACGGGTCGCGCTGCAGCGTGGCGCGCGCGTATATCTACGGTCGCAACCGGCCGAACCTGCACGTGA
TCACCGACGCGACGGTGCTGCGCGTGGGCTTCGACGGCAAGCGCGCGGTCGGCGTCGTGGTGTCGCGCAACGGGCGGGTC
GAGACGTTCGGCGCGCGCGCGGAAGTGGTCCTGTCGGCCGGCGCGTTCAATTCGCCGCAACTGCTGATGTGTTCGGGGAT
CGGCCCGGCCGAGCAATTGCGCCGCCACGGGATCGCGGTCGTGCAGGATGCGCCGGACGTCGGCACGAACCTGATCGACC
ACATCGACTTCATCATCAACACGCGCGTGAATTCGTCGGAGCTGGTCGGCGTGTGCCTGCGCGGCATCGCGAAGATGACG
CCGGCGCTCGCGCGCTATTTCTCGAGCCGCACCGGGATGATGACGAGCAACGTCGCGGAGGCCGGCGGCTTCATCAAGAG
CGATCCGTCGCTCGAGCGTCCCGACTTGCAGCTGCATTTCTGCACGGCGCTCGTCGACGACCACAACCGCAAGATGCACT
GGGGCTTCGGTTATTCGCTGCACGTGTGCGCGCTGCGGCCGTTCAGCCGCGGCACGGTCGCGCTCGCGAGCGGCGATGCG
CGCGACGCGCCGCTGATCGATCCGCGCTTCTTCAGCGATTCACGCGACCTCGACCTGCTGGTGCGCGGCACGCAGGCGAT
GCGCCGCATCCTGTCCCAGGCGCCGCTCGCATCGCAGGGCGGGCGCGAGCTGTACACGCGGCCGGACCAGAGCGAAGCGG
AGCTGCGCGCGACGATCGTCGCGCATGCGGACACGATCTATCACCCGGTCGGCACCTGCCGGATGGGCTCGGATGCGCGC
GCGGTCGTCGATCCGCAACTGCGCGTGCGCGGCGTCGACGGGCTGCGCGTGGTGGATGCGTCGGTGATGCCGACGCTGGT
CGGCGGGAACACGAATGCGCCGTCGGTGATGATCGGCGAACGCGCGGCGGACTTCATCGTCGCCACGCGCAAGGGTGGCG
TGCCGCGCGGCGAGGCTGCGGCTGCGGTGCACGGCCGCTAG

Upstream 100 bases:

>100_bases
GCCGCGCACGGGACAGCCGTTCCTACAATGCCCGAACACAAGAGGAGACGCGGTCGCGCGGCGGTGCCGGGCGCCTTCCA
TGACACGACGGAGACGTGAC

Downstream 100 bases:

>100_bases
GCGCGGCGCGCGCACGGCACGGCGGCCATGCTCAGGCAAACGTATCGACGAGGCCGGCGCGGCTCGCGCGCGCGGCGACC
GGTGCGGATTGCGCGGCGTC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPYKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQEVLPYFRRAEGNERGADEWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVVVSRNGRV
ETFGARAEVVLSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRRILSQAPLASQGGRELYTRPDQSEAELRATIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVDGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGGVPRGEAAAAVHGR

Sequences:

>Translated_546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPYKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQEVLPYFRRAEGNERGADEWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVVVSRNGRV
ETFGARAEVVLSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRRILSQAPLASQGGRELYTRPDQSEAELRATIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVDGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGGVPRGEAAAAVHGR
>Mature_546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPYKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQEVLPYFRRAEGNERGADEWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVVVSRNGRV
ETFGARAEVVLSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRRILSQAPLASQGGRELYTRPDQSEAELRATIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVDGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGGVPRGEAAAAVHGR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=42.6168224299065, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1786503, Length=535, Percent_Identity=39.0654205607477, Blast_Score=337, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17532301, Length=568, Percent_Identity=36.9718309859155, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI45549471, Length=587, Percent_Identity=38.6712095400341, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI45551458, Length=587, Percent_Identity=38.6712095400341, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24642042, Length=572, Percent_Identity=37.0629370629371, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24642048, Length=583, Percent_Identity=36.8782161234991, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24642055, Length=581, Percent_Identity=35.2839931153184, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24642037, Length=578, Percent_Identity=36.8512110726644, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=35.8260869565217, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=35.3765323992995, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24642035, Length=581, Percent_Identity=35.6282271944923, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24642059, Length=574, Percent_Identity=33.9721254355401, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI18859995, Length=571, Percent_Identity=35.9019264448336, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI17137792, Length=566, Percent_Identity=33.7455830388693, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI18859993, Length=576, Percent_Identity=32.9861111111111, Blast_Score=267, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642051, Length=572, Percent_Identity=30.2447552447552, Blast_Score=239, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24642057, Length=588, Percent_Identity=30.2721088435374, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645930, Length=578, Percent_Identity=28.3737024221453, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58645; Mature: 58645

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPYKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEECHHCC
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNERGADEWHGADGPLTVSDLRFRNPFSERFIQAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCHHCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVVVSRNGRV
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
ETFGARAEVVLSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEEECCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHEEE
TRVNSSELVGVCLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCEEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDARDAPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VRGTQAMRRILSQAPLASQGGRELYTRPDQSEAELRATIVAHADTIYHPVGTCRMGSDAR
EECHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHEEEEEEEECCHHCCCCCCEECCCCCC
AVVDPQLRVRGVDGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGGVPRGEAA
EEECCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
AAVHGR
CCCCCC
>Mature Secondary Structure
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPYKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEECHHCC
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNERGADEWHGADGPLTVSDLRFRNPFSERFIQAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCHHCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVVVSRNGRV
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
ETFGARAEVVLSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEEECCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHEEE
TRVNSSELVGVCLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCEEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDARDAPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VRGTQAMRRILSQAPLASQGGRELYTRPDQSEAELRATIVAHADTIYHPVGTCRMGSDAR
EECHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHEEEEEEEECCHHCCCCCCEECCCCCC
AVVDPQLRVRGVDGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGGVPRGEAA
EEECCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
AAVHGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]