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The map label for this gene is slt [H]

Identifier: 116691052

GI number: 116691052

Start: 3347003

End: 3348955

Strand: Reverse

Name: slt [H]

Synonym: Bcen2424_3034

Alternate gene names: 116691052

Gene position: 3348955-3347003 (Counterclockwise)

Preceding gene: 116691054

Following gene: 116691051

Centisome position: 96.13

GC content: 67.64

Gene sequence:

>1953_bases
ATGTCGAACAGCCTTTTCCGAGTATATCGCGCGGCCGCACTGGCGCTTTCCGCCGCCGCGCTCGTCGCGGGCACGGCCGC
ATGCGCCGCACCGAACGACGACACACTCGCCGGGGATGACCAGATCTTCGTGCAGCTTCGCGAAGCCGCACGCCGCAACG
ACGCCGCGAAGGCCGCGCAGCTCGCGTCGATGATCCCGAACTATCCGGTGCCGTCCTACGTCGAGTATTTCCAGATCAAG
CCGCAGCTGTTCGATTCGACGGGCCGCGCGCGCGTCGATGCGCCGGACGCGCCCGTGCAGTCGTTCCTGCAGCGCTACGA
TGGCCAGGCGATCGCCGATCGCCTGCGCAACGACTACCTGCTCGTGCTCGGCGCGCGCCACGACTGGCGCAATTTCGACG
ACCAGTACAAGCGCTTCGTGCTCGACGACGACACGCAGGTCAAGTGCTATGCGCTCGAATCGCGCGCGGCGCGCAACGAG
AACGTCGCCGATGCGGCGCGCGCGCTGCTCGTCGAGCCGAAGAACTACGGCGATGCGTGCGTCGACCTGATCACCGCGCT
CACCGTCAACCAGCAGTTCACGAGCGACGACGTGTGGCAGCAGGCGCGCCTCGCGTACGAGCAGAACTACACGACGCTCG
GCGGCAAGATCGTCGACGCGCTCGGCCCGCGCCCCGTTGGCTTCGACCAGGCGACGAGCGCGCCGCCGCTGTATCTCGCG
CGCGGCGTCGGGCGTGACGCCACGTCGCATCAGCTCGCGCTGATCGCGCTCGGCCGCATGGCGCGCAACGATCCGGACGC
CGCCGCCGGGATGCTGACGACGGTCGCGTCTTCGCTGACCAAGCAGGAACAGGCGATCGCGTGGGGCGCGATCGGCTACC
AGGGCGCGATCAAGCGTTCGCCGATGGCGTCGGTCTGGTATGCGAAATCGGCGAACGCGCCGCTGTCGAACCCGGGCTAC
GAATGGCGCACGCGCGCCGCGCTGCTCGCCGGCAACTGGCCGATGGTGCGCTGGTCGATCGAGCAGATGCCGCCGTCGCT
GCGCAACGATCCGGCGTGGATCTACTGGCACGCGCGTGCGCTCAAGCAGAGCGGCGACACGCTGCAGGCGAACCAGGAAT
TCGAGCAGGTCGCGGGCCAGTTCAACTTCTACGGTCAGTTGGCCGGCGAAGAGCTCGGCCAGCGCACGACGATCCCGCCG
CGCACGAAGGTGAGCGACGCCGAGATCGACGCGATGGGCAAGATCCCGGGCTTCGCGCTCGCGCAGCGTTTCTACGCGCT
GAACCTGCGCCTCGAAGGCAATCGCGAATGGAACTGGCCGCTGCGCGGGATGACCGATCGCCAGCTGCTCGCGGCCGCCG
AATACGGCAAGCGCGTCGAGCTGCTCGACCGCACGGTCAACACGGCCGACCGCACGAAGGCCGAGCACGACTTCACGCTG
CGCTATCCGTCGCCGTACCGCACGACCGTCGAGCGCTACGCGCAGTCGACGGGCCTCGACGTCGAGTGGGCGTACGGGCT
GATCCGCCAGGAATCGCGCTTCATCATCAGCGCGCGTTCGTCGGTGGGCGCGGGCGGCCTGATGCAGCTGATGCCGGCGA
CGGCCCAGATGGTCGCGAAGAAGCTCGGCATGGGCACGATCTCGCGTGCGCAGATGCACGACATCGACACCAACATCCAG
CTCGGCACCTGGTATCTGGCGGACATCTACAACAACTTCGACAGCTCGCCGGTGCTCGCCACGGCCGGCTACAACGCGGG
CCCGGGCCGGCCGCGCCAGTGGCGCCAGGTGTTGACGCAGCCGGTCGAAGGCGCGATCTTCGCGGAGACGATTCCGTTCA
ACGAGACGCGCGACTACGTGAAGAACGTGCTGTCGAATACCGTCTACTACGCGGCGCTCTTCGAGGGCAAACCGCAGTCG
TTGAAGAAACGTCTAGGCATGATCTCGCCCTGA

Upstream 100 bases:

>100_bases
TCGCGACGGGCGCCGGACAGCGTTTTGCGCAGCGCAACCTTCGGGTTCGGCACAGGGTTGCGTGCTATGCTTTCGCTCAC
TTCGTGCTCCATTCAAAACG

Downstream 100 bases:

>100_bases
TACACGAACTGCCGCGCGAGACGCGGCAGTGTTCTTTTTAGGACTCGAACAATGGATCGCCAGACCGTCGCGCTGCTGGG
CGGCACCGGCTTCATCGGCA

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MSNSLFRVYRAAALALSAAALVAGTAACAAPNDDTLAGDDQIFVQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIK
PQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARNE
NVADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLA
RGVGRDATSHQLALIALGRMARNDPDAAAGMLTTVASSLTKQEQAIAWGAIGYQGAIKRSPMASVWYAKSANAPLSNPGY
EWRTRAALLAGNWPMVRWSIEQMPPSLRNDPAWIYWHARALKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPP
RTKVSDAEIDAMGKIPGFALAQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTL
RYPSPYRTTVERYAQSTGLDVEWAYGLIRQESRFIISARSSVGAGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQ
LGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQS
LKKRLGMISP

Sequences:

>Translated_650_residues
MSNSLFRVYRAAALALSAAALVAGTAACAAPNDDTLAGDDQIFVQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIK
PQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARNE
NVADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLA
RGVGRDATSHQLALIALGRMARNDPDAAAGMLTTVASSLTKQEQAIAWGAIGYQGAIKRSPMASVWYAKSANAPLSNPGY
EWRTRAALLAGNWPMVRWSIEQMPPSLRNDPAWIYWHARALKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPP
RTKVSDAEIDAMGKIPGFALAQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTL
RYPSPYRTTVERYAQSTGLDVEWAYGLIRQESRFIISARSSVGAGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQ
LGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQS
LKKRLGMISP
>Mature_649_residues
SNSLFRVYRAAALALSAAALVAGTAACAAPNDDTLAGDDQIFVQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKP
QLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARNEN
VADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLAR
GVGRDATSHQLALIALGRMARNDPDAAAGMLTTVASSLTKQEQAIAWGAIGYQGAIKRSPMASVWYAKSANAPLSNPGYE
WRTRAALLAGNWPMVRWSIEQMPPSLRNDPAWIYWHARALKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPR
TKVSDAEIDAMGKIPGFALAQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTLR
YPSPYRTTVERYAQSTGLDVEWAYGLIRQESRFIISARSSVGAGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQL
GTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSL
KKRLGMISP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=638, Percent_Identity=25.2351097178683, Blast_Score=175, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72043; Mature: 71912

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSLFRVYRAAALALSAAALVAGTAACAAPNDDTLAGDDQIFVQLREAARRNDAAKAAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYL
HHHHCCCCCCCHHHHHHCCCHHHHCCCCCEECCCCCHHHHHHHHHHCCHHHHHHHCCCEE
LVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARNENVADAARALLVEPKNYGDAC
EEEECCCCCCCCCHHHHEEEECCCCCEEEEEECHHHCCCCCHHHHHHHHEECCCCCCHHH
VDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHH
RGVGRDATSHQLALIALGRMARNDPDAAAGMLTTVASSLTKQEQAIAWGAIGYQGAIKRS
HCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PMASVWYAKSANAPLSNPGYEWRTRAALLAGNWPMVRWSIEQMPPSLRNDPAWIYWHARA
CCCCEEEECCCCCCCCCCCCCHHHCEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEEHHH
LKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTKVSDAEIDAMGKIPGFAL
HHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHH
AQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTL
HHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCCEEE
RYPSPYRTTVERYAQSTGLDVEWAYGLIRQESRFIISARSSVGAGGLMQLMPATAQMVAK
ECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQ
HHCCCCCCHHHHHCCCCCEEECCEEHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHC
PVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKKRLGMISP
CCCCCEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCC
>Mature Secondary Structure 
SNSLFRVYRAAALALSAAALVAGTAACAAPNDDTLAGDDQIFVQLREAARRNDAAKAAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYL
HHHHCCCCCCCHHHHHHCCCHHHHCCCCCEECCCCCHHHHHHHHHHCCHHHHHHHCCCEE
LVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARNENVADAARALLVEPKNYGDAC
EEEECCCCCCCCCHHHHEEEECCCCCEEEEEECHHHCCCCCHHHHHHHHEECCCCCCHHH
VDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHH
RGVGRDATSHQLALIALGRMARNDPDAAAGMLTTVASSLTKQEQAIAWGAIGYQGAIKRS
HCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PMASVWYAKSANAPLSNPGYEWRTRAALLAGNWPMVRWSIEQMPPSLRNDPAWIYWHARA
CCCCEEEECCCCCCCCCCCCCHHHCEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEEHHH
LKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTKVSDAEIDAMGKIPGFAL
HHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHH
AQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTL
HHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCCEEE
RYPSPYRTTVERYAQSTGLDVEWAYGLIRQESRFIISARSSVGAGGLMQLMPATAQMVAK
ECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQ
HHCCCCCCHHHHHCCCCCEEECCEEHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHC
PVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKKRLGMISP
CCCCCEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]