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The map label for this gene is flgJ [H]

Identifier: 116691035

GI number: 116691035

Start: 3331017

End: 3332009

Strand: Reverse

Name: flgJ [H]

Synonym: Bcen2424_3017

Alternate gene names: 116691035

Gene position: 3332009-3331017 (Counterclockwise)

Preceding gene: 116691036

Following gene: 116691034

Centisome position: 95.64

GC content: 69.89

Gene sequence:

>993_bases
ATGGCGGCGAACCTTCCGAACGCAAACGACCTCACGCAGCGCTTCGCGCTCGACGTGCAGGGTTTCGACGCGCTGCGCGC
GCAGGCGAAGCAGTCGCCGCAGGCCGGCGCGAAGGCGGTGGCCGGCCAGTTCGACGCGATGTTCACGCAGATGATGCTCA
AGAGCATGCGCGACGCGTCGCCCGACGGCGGGCTGTTCGATTCGCATACGTCGAAGATGTACACGTCGATGCTCGACCAG
CAGCTCGCGCAGCAGATGTCGACACGCGGGATCGGCGTCGCCGACGCGCTGATGAAGCAGTTGCTGCGCAATGCGGGCGC
CGGGGCGGGCAGTGATACGGCGGCCGACGTCGGTGCCGGCGGCATGGGCGGCATGGGCGCCGGCGGCCTCGGCACGGCCG
GCAACGAAGGCAGCCTCGCCGCGATGAACGCGATGGCGAGGGCCTATGCGAACGCGGCGAACAACGGCGGGCTCGCCGGC
GCGCGCGGCTATTCGGCCGGCAGCGCGCTGACCCCGCCGGTCAAGGGCGCGAGCGGCGTGCAGGACGCGGACGCGTTCGT
CGACCGGCTCGCGGCCCCGGCGCAGGCGGCCAGCGCGACGACCGGCATCCCGGCGCGCTTCATCGTCGGCCAGGCCGCGC
TCGAATCGGGCTGGGGCAAGCGCGAGATCCGCGCGGCGGACGGCTCGACCAGCTACAACGTGTTCGGCATCAAGGCGAAC
AAGGGCTGGACCGGCCGCACCGTGTCGGCGCTGACCACCGAATACGTGAACGGCACGCCGCGCCGCGTGGTCGCGAAATT
CCGCGCGTACGACTCGTACGAGCACGCGATGACCGATTACGCGAACCTGCTGAAGAACAACCCGCGCTACGCGGGCGTGC
TGAGCGCGAGCCGCAGCGTCGAGGGCTTCGCGCACGGCATGCAGAAGGCCGGTTACGCAACCGACCCGAATTACGCGAAG
AAGCTGATCTTGATCATGCAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
GGCGCTGAACACGCTCGGCGCGACGCCCGCGGACCTGATGTCGATCCTGCAGGCGATGAAGGCGGCCGGCGCGCTGCGCG
CCGACCTGGAGGTGATCTAA

Downstream 100 bases:

>100_bases
CGGGCCCGCCCGTCATCGGTTCGTCCCCCTTTCGGACCGCGCCCGGCAGCCCGCCGCGCGCGGTTTCAACGTTTGCGCGA
AATAAATCGCCGTTTCGATC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAANLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQ
QLAQQMSTRGIGVADALMKQLLRNAGAGAGSDTAADVGAGGMGGMGAGGLGTAGNEGSLAAMNAMARAYANAANNGGLAG
ARGYSAGSALTPPVKGASGVQDADAFVDRLAAPAQAASATTGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKAN
KGWTGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAHGMQKAGYATDPNYAK
KLILIMQQIG

Sequences:

>Translated_330_residues
MAANLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQ
QLAQQMSTRGIGVADALMKQLLRNAGAGAGSDTAADVGAGGMGGMGAGGLGTAGNEGSLAAMNAMARAYANAANNGGLAG
ARGYSAGSALTPPVKGASGVQDADAFVDRLAAPAQAASATTGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKAN
KGWTGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAHGMQKAGYATDPNYAK
KLILIMQQIG
>Mature_329_residues
AANLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQQ
LAQQMSTRGIGVADALMKQLLRNAGAGAGSDTAADVGAGGMGGMGAGGLGTAGNEGSLAAMNAMARAYANAANNGGLAGA
RGYSAGSALTPPVKGASGVQDADAFVDRLAAPAQAASATTGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKANK
GWTGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAHGMQKAGYATDPNYAKK
LILIMQQIG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=316, Percent_Identity=41.1392405063291, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34014; Mature: 33882

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDAS
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDGGLFDSHTSKMYTSMLDQQLAQQMSTRGIGVADALMKQLLRNAGAGAGSDTAADVGAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCC
GMGGMGAGGLGTAGNEGSLAAMNAMARAYANAANNGGLAGARGYSAGSALTPPVKGASGV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QDADAFVDRLAAPAQAASATTGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKAN
CHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECC
KGWTGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSV
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
EGFAHGMQKAGYATDPNYAKKLILIMQQIG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AANLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDAS
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDGGLFDSHTSKMYTSMLDQQLAQQMSTRGIGVADALMKQLLRNAGAGAGSDTAADVGAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCC
GMGGMGAGGLGTAGNEGSLAAMNAMARAYANAANNGGLAGARGYSAGSALTPPVKGASGV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QDADAFVDRLAAPAQAASATTGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKAN
CHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECC
KGWTGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSV
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
EGFAHGMQKAGYATDPNYAKKLILIMQQIG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]