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The map label for this gene is gcvA [H]

Identifier: 116691030

GI number: 116691030

Start: 3324150

End: 3325034

Strand: Direct

Name: gcvA [H]

Synonym: Bcen2424_3012

Alternate gene names: 116691030

Gene position: 3324150-3325034 (Clockwise)

Preceding gene: 116691020

Following gene: 162227931

Centisome position: 95.41

GC content: 69.94

Gene sequence:

>885_bases
ATGGCACGCGATCTCCCACCCTTTTCCGCGCTTCGGGCCTTTGAAGCCGCGGCACGACATGAAAGCTTCAGCGCCGCCGG
CGACGAGCTGCATGTGACTCACGGTGCCATCAGCCGGCAGATCGCCGGGTTCGAGGCGTGGCTCGGCAAGCCGGTGTTTC
ACCGCTACGGCAAGCGCGTGAAGCTCACCGACGAAGGCCGCCGCTACCTGGACACCGTGCGCGCGGCCTTCGACAGCATC
GCGCTCGCCACCGAACAGTTGCGCAACAGCGGCGCGGCGCGCGTGCTGCGCATCAACGCGCTGCCGACCTTCGCGATGAA
ATGGCTGCTGCCGCGCCTGTCGCGGTTCCAGCGCGACGTGCCGAACGTCGAGCTGAAGCTGTCGACGTCGAATGCGCCAC
TCGACACGCTGAACGGCTTCGACGTCGCGATCCGCCGCGGCCCCGGCCACTGGCCGAACTGCACGAGCGGTCACTTCCTC
GACGAAAGCGTGATTCCGGTGTGCAGCCCGGCGCTGCTCAAGCGGGCGCCGATCACGCGCGCGGACGATCTCGCGCGGCA
CGTGCTACTGCATTCGGATACGCGGCCGGAAGGCTGGCGCGACTGGTTCGCGGCGGCCGGCGTCGCGATGAAGGGGCGCA
AGCGGCAGTCGTTCGATCACTTCTACCTGGCGCTGCAGGCGGCCGTCGACGGGCTCGGCGTCGCGCTCGGCCCGCTGCCG
CTGATCGACGACGAGCTCGCGAGCGGGCGCCTGGTGATGCCGCTCGACGGGCCGCGCATCGCGACGCGCAGCTACTGGTG
GATCGCGCCGGGTGCGCCGGCCGACGATCCGCTCGTCGCGCAGTTCTGCGCGTGGCTGCAGGGGCAGTCGAATACCGGCG
CGTGA

Upstream 100 bases:

>100_bases
TGCATGGGGTCCTCATCAAACGGTGAACCGAGGTTACCGCCGTTCAGGATTCCGGCAAAACGATTTTATCGGTGCGCCCT
TGTGATCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
GGTGAGCACAACGGCGGTATGCACAACGACGGGCGGCATTGCACCGCCCGCTCCCGAATCAGACCACCGGGCCCGGTTCC
TTTTCCTTGCGCAGGATCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRNSGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLNGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVAMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLDGPRIATRSYWWIAPGAPADDPLVAQFCAWLQGQSNTGA

Sequences:

>Translated_294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRNSGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLNGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVAMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLDGPRIATRSYWWIAPGAPADDPLVAQFCAWLQGQSNTGA
>Mature_293_residues
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSIA
LATEQLRNSGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLNGFDVAIRRGPGHWPNCTSGHFLD
ESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVAMKGRKRQSFDHFYLALQAAVDGLGVALGPLPL
IDDELASGRLVMPLDGPRIATRSYWWIAPGAPADDPLVAQFCAWLQGQSNTGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=35.2941176470588, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=30.2013422818792, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=32.6460481099656, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=29.8305084745763, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=28, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=31.7365269461078, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=270, Percent_Identity=25.5555555555556, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32319; Mature: 32188

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRNSGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLNGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRAPITR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVAMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLDGPRIATRSYWWIAPGAPADDPLVAQFCAWLQGQSNTGA
CCCHHHCCCEEEEECCCCEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRNSGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLNGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRAPITR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVAMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLDGPRIATRSYWWIAPGAPADDPLVAQFCAWLQGQSNTGA
CCCHHHCCCEEEEECCCCEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]