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The map label for this gene is amtB [H]

Identifier: 116690885

GI number: 116690885

Start: 3164096

End: 3165598

Strand: Reverse

Name: amtB [H]

Synonym: Bcen2424_2865

Alternate gene names: 116690885

Gene position: 3165598-3164096 (Counterclockwise)

Preceding gene: 116690886

Following gene: 116690884

Centisome position: 90.86

GC content: 68.53

Gene sequence:

>1503_bases
ATGCGAAAACTTCTGATGTCCCTGCTGATGGCCGGTTCGCTGATCGCGACCGGCGTCGGCTCCGCGCTCGCCGACGACGC
GGCGTCCGCCGCGGCTGGTTCCGCGCCCGCCGCCACCGCTTCCGACGCATCGGCCGCCGCTGCACCGGCCGCTTCGGCGC
CGGCCGCCGACGCATCCGCCGCTGCCGCACCGGCTTCGGGCACGGCCGACGCATCGGCTGCCGCCGCCGCGTCCGCGCCG
GCCGCCCCGGCCGCGCCCACCGCCCCGTTCTCGGTCGATTCGTCGAAGATCAGCGCGGGTGACACGGCATGGATGCTGAC
GTCCACCGCGCTCGTGCTGTTCATGACGATCCCCGGCCTCGCGCTGTTCTACGCGGGCATGGTCCGCAAGAAGAACGTGC
TCGCGACCGTGATGCAGAGCTTCGCGATCACCGCGGTGATCACGGTGCTGTGGACGGTGGTCGGCTACAGCCTCGCGTTC
ACGCCGGGCAACGGCTTCATCGGCGGGCTGTCGCGTGCGTTCCTGCACGGCATGAACTACATCAAGGGCGACAAGGCGAC
GACGCTGACCGTCAGTCACCTCGCGTCGACGATTCCGGAATCGGTCTACTTCGTCTACCAGATGACGTTCGCGATCATCA
CGCCGGCGCTGATCTGCGGCGCGTTCGCCGACCGGATGAAGTTCTCGGCGATGCTCGTGTTCATGACGCTCTGGTCGCTG
ATCGTCTATGTGCCGATCGCGCACATGGTGTGGGAGCCGACCGGCTGGCTGTCGGCCGACGGCGTGCTCGACTTCGCGGG
CGGCACGGTGGTGCACATCAACGCCGGTATCGCGGGCCTCGTGTCGTGCCTCGTGCTCGGCAAGCGCGTCGGCTACGGCC
GTGAATCGATGGCGCCGCACAACCTGGTGCTGACGATGATCGGCGGCTCGATGCTGTGGGTCGGCTGGTTCGGCTTCAAC
GCGGGCTCCGCGGTCGCGGCCGACGGCCGTGCCGGCTTCGCGATGCTGACCACGCAAGTGGCGACCGCCTGCGCGGCGCT
CGGCTGGATGTTTGCCGAGTGGATCGCGAAGGGCAAGCCGTCGGTGCTCGGCATCGTGTCGGGCGCGGTCGCGGGTCTCG
TGGCGATCACGCCGGCCGCCGGTTTCGTCGGCGTGGCGGGCGCACTGGTGATCGGTATCGCGGCGGGCGTCGTCTGCTTC
TGGTCGGCTACCTGGCTCAAGTCCAAGCTCGGCTACGACGATTCGCTCGACGCGTTCGGCGTGCACGGCATCGGCGGGAT
TCTCGGCGCACTGCTGACGGGCGTATTCGCGGTCAAGGACATCGGCGGCTTCGACGGCAGCCTGCTGCTGCAGGCCAAGG
GCGTGCTGATCACGCTGGTCTACAGCGGCGTGCTGAGCTTCGTGCTGCTGAAGCTGATCGACCTGACGATCGGCCTGCGC
GTGACCGAAGAGGAAGAGCGCGAAGGTCTCGACGTGATCCTGCACGGCGAGCACGTGGAATAA

Upstream 100 bases:

>100_bases
GTCACGCCGATCGAACAGGTGATCCGGATTCGCACCGGGGAGACAGGCGCGGACGCGCTGTAACAGAACAGACAAGACAA
GCGACAAGAGGAAACCCAAG

Downstream 100 bases:

>100_bases
GTCACACGAATCAACGGCGGGCCGCCGCGACGACGGCGCCCGCCCCGAATGAGCGCAGCGACTTCGGCCCGCCTACCCGG
CGGGCTTTTTTTCTCCTGCC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MRKLLMSLLMAGSLIATGVGSALADDAASAAAGSAPAATASDASAAAAPAASAPAADASAAAAPASGTADASAAAAASAP
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAF
TPGNGFIGGLSRAFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLVSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGIGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE

Sequences:

>Translated_500_residues
MRKLLMSLLMAGSLIATGVGSALADDAASAAAGSAPAATASDASAAAAPAASAPAADASAAAAPASGTADASAAAAASAP
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAF
TPGNGFIGGLSRAFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLVSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGIGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE
>Mature_500_residues
MRKLLMSLLMAGSLIATGVGSALADDAASAAAGSAPAATASDASAAAAPAASAPAADASAAAAPASGTADASAAAAASAP
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAF
TPGNGFIGGLSRAFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLVSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGIGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=50.2463054187192, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17550182, Length=447, Percent_Identity=27.2930648769575, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=26.0869565217391, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=435, Percent_Identity=27.5862068965517, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=453, Percent_Identity=24.7240618101545, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=36.2790697674419, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6325396, Length=429, Percent_Identity=35.6643356643357, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=32.5358851674641, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=27.0783847980998, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50679; Mature: 50679

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLMSLLMAGSLIATGVGSALADDAASAAAGSAPAATASDASAAAAPAASAPAADASA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AAAPASGTADASAAAAASAPAAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGL
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAFTPGNGFIGGLSRAFLHGMNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
IKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLVSCLVLGKRVGYGRESMAPH
HHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
NLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKP
CEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCFWSATWLKSKLGYDDSLDAFG
CEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VHGIGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCE
VTEEEEREGLDVILHGEHVE
ECCHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MRKLLMSLLMAGSLIATGVGSALADDAASAAAGSAPAATASDASAAAAPAASAPAADASA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AAAPASGTADASAAAAASAPAAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGL
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAFTPGNGFIGGLSRAFLHGMNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
IKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLVSCLVLGKRVGYGRESMAPH
HHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
NLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKP
CEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCFWSATWLKSKLGYDDSLDAFG
CEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VHGIGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCE
VTEEEEREGLDVILHGEHVE
ECCHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]