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The map label for this gene is 116690884

Identifier: 116690884

GI number: 116690884

Start: 3162563

End: 3163852

Strand: Reverse

Name: 116690884

Synonym: Bcen2424_2864

Alternate gene names: NA

Gene position: 3163852-3162563 (Counterclockwise)

Preceding gene: 116690885

Following gene: 116690883

Centisome position: 90.81

GC content: 65.97

Gene sequence:

>1290_bases
ATGGTTCCCCACCTCGTTACCGCGTTGAACGGTCCGCTGCTCGAACTCGAGCAGAAGATCCTCGACGCGACGCCTGCGAT
CGAACGCTGGTTCAGGCTCGAATGGCAGGAACACACCCCGCCGTTCTATTGTTCGGTGGACCTGCGCAATGCCGGCTTCA
AGCTGGCGCCCGTCGACGCGAACCTGTTTCCCGGCGCGTTCAACAATCTGCCGTCCGAAGTGCTGCCGCTCGCCGTGCAG
GCCGCGATGGCCGCGATCGAGAAGATCTGCCCGGACGCGAAGAATCTGCTCGTGATTCCCGAGCTGCCGACCCGCAACGC
GTTCTACCTGGAAAACGTCGCGCGCCTCGCGACGATCATGCGCCAGGCCGGCCTGAACGTGCGCTTCGGCTCGCTCGACC
CGAGCATCACCGACATGACGCCGATCACGCTGGCCGACGGCCAGAAGATCGTGCTCGAACCGCTCGAGCGTTCGCAGCGC
CGCCTCGGCCTGAAGAATTTCGATCCGTGCTCGATCCTGCTGAACAACGACCTGTCGGCCGGCATCCCGGCCGTGCTGGA
AAACCTGCACGAGCAGTACCTGCTGCCGCCGCTGCACGCGGGCTGGGCCGTGCGCCGCAAGTCGACGCACTTCTCGTGCT
ACGACGACGTCGCGAAGAAGTTCGCGAAGATGGTCGGCGTCGATCCGTGGATGGTGAATCCGTATTTCGCGCACGTGGAA
GGCGTCGACTGGCAGGCGCATGAAGGCGAGCAGGCGCTCGCCGACGCGATCGACGGGGTGCTGAAGAAGATCGCGCGCAA
GTATCGCGAATACGGGATCAGCGAGAAGCCGTACGTCGTCGTGAAGGCCGACGCGGGCACCGCGGGCCGTGGCGTGATGA
CCGTGCACGACGCGGCCGAGATCGGCCGCATGACGAAGGCCGAGCGCGCGCAGATGGCTGAATCGAAGGCCGGCCTCGCG
GTGCGCGACGTGATCGTGCAGGAAGGCGTCTACACGTTCGAGCGCGTCGGCGACGAAGTGGCCGAGCCCGTCGTGTACAT
GATCGACCGCTACGTGGTCGGCGGCTTCTACCGCACGCACGGCGGCCGCGAGCGCGACCAGAACCTGAACGCGCCCGGCA
TGCACTACGTGCCGCTCGGCTTCGAGCACACCGCGCTGCCGGATGCAGGCGCGAAGCCGGGCGCCGCGCCGCCGAACCGT
TTCTACATGTACGGCGTCGTCGCGCGGCTGTCGCTGATCGCGTCGTCGATCGAACTGGAAAAGACCGATCCCGAAGCCAT
CCAGGTGTAA

Upstream 100 bases:

>100_bases
AGCCGAAAAGTTCCTCGATATCCTTGCGAAGCCGGGAACAATCCCCAAATGGGCAAGGCAATTGCTCTACAATCTTCATT
CTCCCGCTCGCGAGTGATTC

Downstream 100 bases:

>100_bases
CGGCTTCGCTACGTACAGGACCCGCATGGACATTCTTTTTATCGCCGACCCGCTCGATCATTTCAAGATCTACAAGGATT
CGACCTACGCGATGATGGCG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVPHLVTALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPSEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSITDMTPITLADGQKIVLEPLERSQR
RLGLKNFDPCSILLNNDLSAGIPAVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMVNPYFAHVE
GVDWQAHEGEQALADAIDGVLKKIARKYREYGISEKPYVVVKADAGTAGRGVMTVHDAAEIGRMTKAERAQMAESKAGLA
VRDVIVQEGVYTFERVGDEVAEPVVYMIDRYVVGGFYRTHGGRERDQNLNAPGMHYVPLGFEHTALPDAGAKPGAAPPNR
FYMYGVVARLSLIASSIELEKTDPEAIQV

Sequences:

>Translated_429_residues
MVPHLVTALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPSEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSITDMTPITLADGQKIVLEPLERSQR
RLGLKNFDPCSILLNNDLSAGIPAVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMVNPYFAHVE
GVDWQAHEGEQALADAIDGVLKKIARKYREYGISEKPYVVVKADAGTAGRGVMTVHDAAEIGRMTKAERAQMAESKAGLA
VRDVIVQEGVYTFERVGDEVAEPVVYMIDRYVVGGFYRTHGGRERDQNLNAPGMHYVPLGFEHTALPDAGAKPGAAPPNR
FYMYGVVARLSLIASSIELEKTDPEAIQV
>Mature_429_residues
MVPHLVTALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPSEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSITDMTPITLADGQKIVLEPLERSQR
RLGLKNFDPCSILLNNDLSAGIPAVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMVNPYFAHVE
GVDWQAHEGEQALADAIDGVLKKIARKYREYGISEKPYVVVKADAGTAGRGVMTVHDAAEIGRMTKAERAQMAESKAGLA
VRDVIVQEGVYTFERVGDEVAEPVVYMIDRYVVGGFYRTHGGRERDQNLNAPGMHYVPLGFEHTALPDAGAKPGAAPPNR
FYMYGVVARLSLIASSIELEKTDPEAIQV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47365; Mature: 47365

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLVTALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPSEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPSITDMTPITLADGQKIVLEPLERSQRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPAVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMVNPYFAHVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDWQAHEGEQALADAIDGVLKKIARKYREYGISEKPYVVVKADAGTAGRGVMTVHDAAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEHHHHH
IGRMTKAERAQMAESKAGLAVRDVIVQEGVYTFERVGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
GGRERDQNLNAPGMHYVPLGFEHTALPDAGAKPGAAPPNRFYMYGVVARLSLIASSIELE
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KTDPEAIQV
CCCCHHCCC
>Mature Secondary Structure
MVPHLVTALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPSEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPSITDMTPITLADGQKIVLEPLERSQRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPAVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMVNPYFAHVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDWQAHEGEQALADAIDGVLKKIARKYREYGISEKPYVVVKADAGTAGRGVMTVHDAAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEHHHHH
IGRMTKAERAQMAESKAGLAVRDVIVQEGVYTFERVGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
GGRERDQNLNAPGMHYVPLGFEHTALPDAGAKPGAAPPNRFYMYGVVARLSLIASSIELE
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KTDPEAIQV
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA