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The map label for this gene is rpoN [H]

Identifier: 116690811

GI number: 116690811

Start: 3087596

End: 3089092

Strand: Direct

Name: rpoN [H]

Synonym: Bcen2424_2791

Alternate gene names: 116690811

Gene position: 3087596-3089092 (Clockwise)

Preceding gene: 116690810

Following gene: 116690812

Centisome position: 88.62

GC content: 64.8

Gene sequence:

>1497_bases
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCAGCACGTCCAGCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTCCGGTGACGG
CAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGCCGCCGCTGC
AGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACCCAGGCCGGCCCGCGCGATCGC
GCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGATGGCTACCTGACCGCCACGCTCGACGAAGTACTCGCCGACCT
GCCGCCCGAACTGGAAATCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGGCCGGCGTCG
GCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCCACGCGCACGCTCGCGCTCGAG
ATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAGCGACGACGC
GCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGGCCGACTACG
TCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCGCGCCTGCGG
ATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAGCAGCAGCTGCAGGA
AGCTCGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCGAGCGTCAGA
AGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTACACGAG
TCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTTCTTCGGATC
TCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAGGAGCTGAAG
ACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGCCGCACGGTT
GCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Upstream 100 bases:

>100_bases
CCGCGGCCCTGCCCGCGGCCGGGCGGCGCGCCGCGCGTAATCGCGGATGGCTCAATGCGCCTGGGTCGTGGCAAACTGCC
GGTATGACTCCCTCCTTGCC

Downstream 100 bases:

>100_bases
GCCTGCTGCCTGCCCGGCGGCAGGCGTGTCCCGGCCACCGCGCACACGGGGAACAGGCCGGACGATCTGTCCGCGATACG
CCGCTTCATGCGGCACGGGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDR
ALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVPRLR
INNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHE
STVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
AKYREALKIPAVNLRKSL

Sequences:

>Translated_498_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDR
ALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVPRLR
INNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHE
STVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
AKYREALKIPAVNLRKSL
>Mature_498_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDR
ALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVPRLR
INNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHE
STVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
AKYREALKIPAVNLRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=500, Percent_Identity=45.2, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54398; Mature: 54398

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRV
CCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHE
AADGSLIAQTPPAQNTEPTATNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGR
ECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
SSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDRALVMFLIESLDDDGYLTATL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEEEHHH
DEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
HHHHHCCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEHEECCCCCHHHHHHH
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
KKAGQGWLAELNPEVVPRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFD
HHCCCCCHHHCCCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSRVTTGKYMLTPFGTLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCEE
FKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
AKYREALKIPAVNLRKSL
HHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRV
CCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHE
AADGSLIAQTPPAQNTEPTATNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGR
ECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
SSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDRALVMFLIESLDDDGYLTATL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEEEHHH
DEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
HHHHHCCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEHEECCCCCHHHHHHH
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
KKAGQGWLAELNPEVVPRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFD
HHCCCCCHHHCCCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSRVTTGKYMLTPFGTLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCEE
FKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
AKYREALKIPAVNLRKSL
HHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]