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The map label for this gene is glyS [H]

Identifier: 116690722

GI number: 116690722

Start: 2992462

End: 2994561

Strand: Direct

Name: glyS [H]

Synonym: Bcen2424_2702

Alternate gene names: 116690722

Gene position: 2992462-2994561 (Clockwise)

Preceding gene: 116690721

Following gene: 116690723

Centisome position: 85.89

GC content: 68.81

Gene sequence:

>2100_bases
ATGACGCACAATCATCCCGCCCCCCTGCTCGTCGAACTGCTGACCGAAGAGCTGCCGCCGAAGGCCCTCGCGCGCCTCGG
CGACGCATTCGCCGAAGGCATCGCGCAACGCCTCGCGGCGCGCGACCTCATCGAAGGCGAACTCGTGTTCGAGCGCTACG
CCACGCCGCGCCGCCTCGCGGTCGTCGTGCAGAACGTGCGCGCCGTCGCGCCTGACAAACAGGTCCGCGAAAAGGTCCTG
CCCGTGTCGGTCGCGCTCGACGCCGAAGGCAAGCCGACGGCCCCGCTCGCGAAGAAGCTCGCGGCGCTCGGCCACCCGAA
TGCGTCGATCGCCGATCTCGAACGCGCGCAGGACGGCAAGGCCGAAGCGTTCTTCATCAACTACTCGGCGGCCGGCGCCA
CGCTCGCCGACGGCCTGCAGGCCGCGCTCGACGAAGCGCTCGCGAAGCTGCCGATCCCGAAATTCATGACGTACCAGCGC
CCGGACGGCTCGGACGTGAAGTTCGTGCGCCCGGTGCATCGCCTGACGGTGCTGCACGACGACCGCGTCGTGCCCGTCAC
CGCGTTCGGCGTCGATGCCGGCGACACGACGCTCGGCCACCGCTTCCTGTCCGACGGCCTGGTCGCGATCCAGCATGCCC
GCGCGTATGCCGACACGCTGCGCGACAAGGGCCGCGTGATCGCGCACTTCGCCGATCGCCGCGAAACGATCCGCACGCAG
CTGAACGAACATGCGAACGGCGACACGGTCGTGATGCCCGAGTCGCTGCTCGACGAAGTCACGTCGCTGGTCGAATGGCC
GGTCGTCTACCCGTGCCGCTTCGAGGACGAATTCCTGCAGGTGCCGCAGGAATGCCTGATCCTCACGATGCAGACGAACC
AGAAGTATTTCGCGCTGACCGACGTCGCCGGCAAGCTGCGCTCGCGCTTCCTGATCGTGTCGAACATCGAGACGAAAACG
CCGGGCGAGATCATCGAAGGCAACGAGCGCGTCGTGCGCCCGCGCCTCGCCGATGCAAAGTTCTTCTTCGAGCAGGACAA
GAAGAAGCCGCTCGCCGACCGCGTGCCGCAGCTCGCGAACGTCGTCTATCACAACAAGCTGGGTTCGGCGCTCGCCCGCG
TCGAGCGCCTCGAGGCGCTGGCCGGCGAGATCGCCCCGGCGATCGGCGCCGACACCGCGCACGCGAAGCGCGCCGCGCGC
CTCGCGAAGGCCGATCTGCTGACCGACATGGTCGGCGAATTCCCGGAACTGCAGGGCACGATGGGCACGTACTACGCGCG
CCACGACGGCGAAGCCGACGACGTCGCGCTCGCATGCGCCGAGCACTACCAGCCGCGCTTCTCCGGCGACGCACTGCCGA
CCACGCCGGTGTCGACCGCCGTCGCGCTCGCGGACAAGCTCGAAACGATCGTCGGCATCTGGGGCATCGGCCTCGCGCCG
ACCGGCGAGAAGGACCCGTTCGCGCTGCGCCGTCACGCGCTCGGCGTGCTGCGCCTGCTGCTCGAAAAGCAGTTGCCGCT
CGATCTCGTGTCGCTGTTGCACACGGCCCACGCACGCTTTGCCGGCGTGCCGGGCGTCGCCGAGTCGACGGACGCCATCT
TCGCGTTCTTCATGGATCGCCTGCGCGGCCTGCTGCGCGAGCGCGGCTATTCGGCCGGTGAAATCGACGCGGTGCTGAGC
CTGAACCCGACGCGCATCGACGATCTCGTCGCGCGCCTCGACGCGGTACGCGAATTCACGCGCCTCGCGGAAGCCGAAGC
ACTCGCGGCCGCGAACAAGCGGATCTCGAACATCCTGAAGAAGTCGGAAGGCGGCGCGAACGGCGCGGTGCAGCCGACGC
TGCTCGTCGAGGCCGCCGAGAAGGCGCTGCACGAACAGCTCGCGGCCGTCACGCCGCACGTGCAGTCGCAGCTCGAGGCG
CGTGCGTACACGGGCGCGCTGTCGGCGCTCGCCGCGCTGCGCGTGCCGGTCGACACGTTCTTCAACGACGTGATGGTCAA
CGCGGAAGATCCGGCCCTGCGCGCGAACCGTCTCGCGCTGCTGTCCGCGCTGCACCAGCAGATGAACTGCGTCGCCGACA
TCTCGAAGCTCGCCGCATAA

Upstream 100 bases:

>100_bases
ACCACCGACGCCCAGGACGCCGCGCAGCCGGCATGGGCGCCGCCGCTCAAGGTCGAACGCAAGATCGATCAGGACTGACG
AGACGAGATTCAATCCAACC

Downstream 100 bases:

>100_bases
GGGCCGCACGATGCCGACCAGCCCCAACCGAAAGCTCGTCGTCCTCGACCGGGACGGCGTGATCAACGTCGATTCGGATG
CGTTCATCAAGACGCCCGAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELVFERYATPRRLAVVVQNVRAVAPDKQVREKVL
PVSVALDAEGKPTAPLAKKLAALGHPNASIADLERAQDGKAEAFFINYSAAGATLADGLQAALDEALAKLPIPKFMTYQR
PDGSDVKFVRPVHRLTVLHDDRVVPVTAFGVDAGDTTLGHRFLSDGLVAIQHARAYADTLRDKGRVIAHFADRRETIRTQ
LNEHANGDTVVMPESLLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDVAGKLRSRFLIVSNIETKT
PGEIIEGNERVVRPRLADAKFFFEQDKKKPLADRVPQLANVVYHNKLGSALARVERLEALAGEIAPAIGADTAHAKRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEADDVALACAEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLLEKQLPLDLVSLLHTAHARFAGVPGVAESTDAIFAFFMDRLRGLLRERGYSAGEIDAVLS
LNPTRIDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEA
RAYTGALSALAALRVPVDTFFNDVMVNAEDPALRANRLALLSALHQQMNCVADISKLAA

Sequences:

>Translated_699_residues
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELVFERYATPRRLAVVVQNVRAVAPDKQVREKVL
PVSVALDAEGKPTAPLAKKLAALGHPNASIADLERAQDGKAEAFFINYSAAGATLADGLQAALDEALAKLPIPKFMTYQR
PDGSDVKFVRPVHRLTVLHDDRVVPVTAFGVDAGDTTLGHRFLSDGLVAIQHARAYADTLRDKGRVIAHFADRRETIRTQ
LNEHANGDTVVMPESLLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDVAGKLRSRFLIVSNIETKT
PGEIIEGNERVVRPRLADAKFFFEQDKKKPLADRVPQLANVVYHNKLGSALARVERLEALAGEIAPAIGADTAHAKRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEADDVALACAEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLLEKQLPLDLVSLLHTAHARFAGVPGVAESTDAIFAFFMDRLRGLLRERGYSAGEIDAVLS
LNPTRIDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEA
RAYTGALSALAALRVPVDTFFNDVMVNAEDPALRANRLALLSALHQQMNCVADISKLAA
>Mature_698_residues
THNHPAPLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELVFERYATPRRLAVVVQNVRAVAPDKQVREKVLP
VSVALDAEGKPTAPLAKKLAALGHPNASIADLERAQDGKAEAFFINYSAAGATLADGLQAALDEALAKLPIPKFMTYQRP
DGSDVKFVRPVHRLTVLHDDRVVPVTAFGVDAGDTTLGHRFLSDGLVAIQHARAYADTLRDKGRVIAHFADRRETIRTQL
NEHANGDTVVMPESLLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDVAGKLRSRFLIVSNIETKTP
GEIIEGNERVVRPRLADAKFFFEQDKKKPLADRVPQLANVVYHNKLGSALARVERLEALAGEIAPAIGADTAHAKRAARL
AKADLLTDMVGEFPELQGTMGTYYARHDGEADDVALACAEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAPT
GEKDPFALRRHALGVLRLLLEKQLPLDLVSLLHTAHARFAGVPGVAESTDAIFAFFMDRLRGLLRERGYSAGEIDAVLSL
NPTRIDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEAR
AYTGALSALAALRVPVDTFFNDVMVNAEDPALRANRLALLSALHQQMNCVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=44.7178002894356, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75910; Mature: 75779

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELVFERYATPRRLA
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPDKQVREKVLPVSVALDAEGKPTAPLAKKLAALGHPNASIADLERAQDGK
HHHHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCC
AEAFFINYSAAGATLADGLQAALDEALAKLPIPKFMTYQRPDGSDVKFVRPVHRLTVLHD
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHEEEC
DRVVPVTAFGVDAGDTTLGHRFLSDGLVAIQHARAYADTLRDKGRVIAHFADRRETIRTQ
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LNEHANGDTVVMPESLLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEECCHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCCEEEHH
DVAGKLRSRFLIVSNIETKTPGEIIEGNERVVRPRLADAKFFFEQDKKKPLADRVPQLAN
HHHHHHHHCEEEEECCCCCCCCHHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSALARVERLEALAGEIAPAIGADTAHAKRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEADDVALACAEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLLEKQLPLDLVSLLHTAHARFAGVPGVAESTDAIFAFFMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRGLLRERGYSAGEIDAVLSLNPTRIDDLVARLDAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEARAYTGALSALAALRVPVDTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FNDVMVNAEDPALRANRLALLSALHQQMNCVADISKLAA
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THNHPAPLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELVFERYATPRRLA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPDKQVREKVLPVSVALDAEGKPTAPLAKKLAALGHPNASIADLERAQDGK
HHHHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCC
AEAFFINYSAAGATLADGLQAALDEALAKLPIPKFMTYQRPDGSDVKFVRPVHRLTVLHD
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHEEEC
DRVVPVTAFGVDAGDTTLGHRFLSDGLVAIQHARAYADTLRDKGRVIAHFADRRETIRTQ
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LNEHANGDTVVMPESLLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEECCHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCCEEEHH
DVAGKLRSRFLIVSNIETKTPGEIIEGNERVVRPRLADAKFFFEQDKKKPLADRVPQLAN
HHHHHHHHCEEEEECCCCCCCCHHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSALARVERLEALAGEIAPAIGADTAHAKRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEADDVALACAEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLLEKQLPLDLVSLLHTAHARFAGVPGVAESTDAIFAFFMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRGLLRERGYSAGEIDAVLSLNPTRIDDLVARLDAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEARAYTGALSALAALRVPVDTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FNDVMVNAEDPALRANRLALLSALHQQMNCVADISKLAA
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA