Click here to switch to the map view.

The map label for this gene is cobQ [H]

Identifier: 116690466

GI number: 116690466

Start: 2718675

End: 2720210

Strand: Reverse

Name: cobQ [H]

Synonym: Bcen2424_2446

Alternate gene names: 116690466

Gene position: 2720210-2718675 (Counterclockwise)

Preceding gene: 116690471

Following gene: 116690465

Centisome position: 78.08

GC content: 71.09

Gene sequence:

>1536_bases
ATGCCGCCTTTGCTTCGCGACACCTTTCACACGATGAATGCACCCGAGCCGCGGCCGCGCGGCACGCTGATGATCCAGGG
CACGACGTCCGACGCGGGCAAGAGCACGCTCGTCGCGGGCCTGTGCCGCCTCGCGCGCCGCGGCGGCGCGCGCGTGGCGC
CGTTCAAGCCGCAGAACATGGCGCTCAACAGCGCGGTGACGGCAGACGGCGGCGAGATCGGCCGCGCACAGGCGTTGCAG
GCGCTGGCCGCCGGCGTCGCGCCGCATACGGATTTCAATCCGGTGCTGCTGAAGCCGACGAGCGACCGCGGCGCGCAGGT
GATCATTCACGGCCAGGCGCGCATGAACCTCGACGCGCGCGCGTATCACGACTACAAGCCGGTTGCGTTCGATGCGGTGC
TCGAGTCGTATGCGCGCCTGCGCGCCGGGTACGACACGGTGATCGTCGAAGGCGCCGGCAGCCCGGCCGAGATCAACCTG
CGCGAAGGCGACATCGCGAACATGGGCTTTGCCGAGCGCGTCGACTGTCCGGTCGTGCTCGTCGCCGACATCGATCGCGG
CGGCGTGTTCGCGCATCTGGTCGGCACGCTCGCGTGCCTGTCGGACAGCGAGCGCGCGCGCGTGCGCGGCTTCGTGATCA
ATCGCTTTCGCGGCGACCTCAAGCTGCTCGAACCGGGACTCGACTGGCTGCGCGCGCAGACGGACAAGCCGGTGTTCGGC
GTGCTGCCGTATCTGCACGGGCTGCTGCTCGACGCGGAGGACATGCTGCCCGCGCAGGCGCGCAGCGCCGCCGCTCGCAG
CGATGCCGGCGTGTTGCGCGTCGTCGTGCCCGCGCTGCCGCGCATCAGCAACCACACCGATTTCGATCCGCTGCGCGCGC
ATCCGCAGGTCGAATTCACGTATTGGAAGAGCGGCCCCGTACCCGACGCCGATCTGCTGATCCTGCCCGGTTCGAAGAGC
GTGCAGCGCGACCTCGCGTGGCTGCGCGATGCGGGCTGGGACGCGCTGATCCGGCGCCATCTGCGCTACGGCGGCAAGGT
GATCGGCATCTGCGGCGGCATGCAGATGCTCGGCCGCACGCTCGACGATCCGCTCGGCCTCGAAGGCGCGCCCGGCAGCG
TGCCGGGGCTCGGGCTGCTCGATTTCGACACGACGCTGCAACCCGACAAGACGCTGAAGAACGTGACCGGGCAACTCGCG
CTGCCGGGCGCGGCCGCCGTGCGCGGCTACGAGATCCACATGGGCGACACGCGCGGGCCGGCGCTCGAGACGCCCGCGCT
GATGCTGGCCGCCGATATCGCGCGGGAGGGCGTGCGTCCCGACGGCGCGCTGTCGGCCGACGGCCAGATTCTCGCGACCT
ACGTGCACGGGTTGTTCGACGCACCCGACGCCTGCGCGGCGCTGCTCGCGTGGGCGGGGCTCGACGGCGCGGCCCGCATC
GACTACCCGGCGCTGCGCGAGGCTTCGCTGGAGCGGCTGGCCGACACGTTCGCCGCGCACCTCGACCTCGACGCGCTGTA
CGCCGAGTTTCGTTGA

Upstream 100 bases:

>100_bases
TGCGCGCTCTTGCCCGAGCGCGCGCCGCCGAGGACGAAGGTGAGGTCGTGCGGAATCATCGCGTGATTGTAACGGCGCGG
GCGACCCGGCGCGGGCGATA

Downstream 100 bases:

>100_bases
GGCCGGCCCTCGCTTTTTCCGCCCGATCGCGTACAACAGAGGAGGGCGGAGGTCCGCCCGAAGGAGGGCGACGATGAGGG
CGCGACGGTGGTGGGGCGTA

Product: cobyric acid synthase

Products: ADP; Dethiobiotin; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPPLLRDTFHTMNAPEPRPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGARVAPFKPQNMALNSAVTADGGEIGRAQALQ
ALAAGVAPHTDFNPVLLKPTSDRGAQVIIHGQARMNLDARAYHDYKPVAFDAVLESYARLRAGYDTVIVEGAGSPAEINL
REGDIANMGFAERVDCPVVLVADIDRGGVFAHLVGTLACLSDSERARVRGFVINRFRGDLKLLEPGLDWLRAQTDKPVFG
VLPYLHGLLLDAEDMLPAQARSAAARSDAGVLRVVVPALPRISNHTDFDPLRAHPQVEFTYWKSGPVPDADLLILPGSKS
VQRDLAWLRDAGWDALIRRHLRYGGKVIGICGGMQMLGRTLDDPLGLEGAPGSVPGLGLLDFDTTLQPDKTLKNVTGQLA
LPGAAAVRGYEIHMGDTRGPALETPALMLAADIAREGVRPDGALSADGQILATYVHGLFDAPDACAALLAWAGLDGAARI
DYPALREASLERLADTFAAHLDLDALYAEFR

Sequences:

>Translated_511_residues
MPPLLRDTFHTMNAPEPRPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGARVAPFKPQNMALNSAVTADGGEIGRAQALQ
ALAAGVAPHTDFNPVLLKPTSDRGAQVIIHGQARMNLDARAYHDYKPVAFDAVLESYARLRAGYDTVIVEGAGSPAEINL
REGDIANMGFAERVDCPVVLVADIDRGGVFAHLVGTLACLSDSERARVRGFVINRFRGDLKLLEPGLDWLRAQTDKPVFG
VLPYLHGLLLDAEDMLPAQARSAAARSDAGVLRVVVPALPRISNHTDFDPLRAHPQVEFTYWKSGPVPDADLLILPGSKS
VQRDLAWLRDAGWDALIRRHLRYGGKVIGICGGMQMLGRTLDDPLGLEGAPGSVPGLGLLDFDTTLQPDKTLKNVTGQLA
LPGAAAVRGYEIHMGDTRGPALETPALMLAADIAREGVRPDGALSADGQILATYVHGLFDAPDACAALLAWAGLDGAARI
DYPALREASLERLADTFAAHLDLDALYAEFR
>Mature_510_residues
PPLLRDTFHTMNAPEPRPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGARVAPFKPQNMALNSAVTADGGEIGRAQALQA
LAAGVAPHTDFNPVLLKPTSDRGAQVIIHGQARMNLDARAYHDYKPVAFDAVLESYARLRAGYDTVIVEGAGSPAEINLR
EGDIANMGFAERVDCPVVLVADIDRGGVFAHLVGTLACLSDSERARVRGFVINRFRGDLKLLEPGLDWLRAQTDKPVFGV
LPYLHGLLLDAEDMLPAQARSAAARSDAGVLRVVVPALPRISNHTDFDPLRAHPQVEFTYWKSGPVPDADLLILPGSKSV
QRDLAWLRDAGWDALIRRHLRYGGKVIGICGGMQMLGRTLDDPLGLEGAPGSVPGLGLLDFDTTLQPDKTLKNVTGQLAL
PGAAAVRGYEIHMGDTRGPALETPALMLAADIAREGVRPDGALSADGQILATYVHGLFDAPDACAALLAWAGLDGAARID
YPALREASLERLADTFAAHLDLDALYAEFR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.3.3 [C]

Molecular weight: Translated: 54561; Mature: 54430

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLLRDTFHTMNAPEPRPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGARVAPFKPQNM
CCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
ALNSAVTADGGEIGRAQALQALAAGVAPHTDFNPVLLKPTSDRGAQVIIHGQARMNLDAR
EECCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCEECCCHH
AYHDYKPVAFDAVLESYARLRAGYDTVIVEGAGSPAEINLREGDIANMGFAERVDCPVVL
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEE
VADIDRGGVFAHLVGTLACLSDSERARVRGFVINRFRGDLKLLEPGLDWLRAQTDKPVFG
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEECCCHHHHHHCCCCCHHH
VLPYLHGLLLDAEDMLPAQARSAAARSDAGVLRVVVPALPRISNHTDFDPLRAHPQVEFT
HHHHHHHHHHCHHHCCCHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCEEEE
YWKSGPVPDADLLILPGSKSVQRDLAWLRDAGWDALIRRHLRYGGKVIGICGGMQMLGRT
EECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCHHHHHHH
LDDPLGLEGAPGSVPGLGLLDFDTTLQPDKTLKNVTGQLALPGAAAVRGYEIHMGDTRGP
CCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCEECCCHHHEEEEEEEECCCCCC
ALETPALMLAADIAREGVRPDGALSADGQILATYVHGLFDAPDACAALLAWAGLDGAARI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
DYPALREASLERLADTFAAHLDLDALYAEFR
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
PPLLRDTFHTMNAPEPRPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGARVAPFKPQNM
CCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
ALNSAVTADGGEIGRAQALQALAAGVAPHTDFNPVLLKPTSDRGAQVIIHGQARMNLDAR
EECCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCEECCCHH
AYHDYKPVAFDAVLESYARLRAGYDTVIVEGAGSPAEINLREGDIANMGFAERVDCPVVL
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEE
VADIDRGGVFAHLVGTLACLSDSERARVRGFVINRFRGDLKLLEPGLDWLRAQTDKPVFG
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEECCCHHHHHHCCCCCHHH
VLPYLHGLLLDAEDMLPAQARSAAARSDAGVLRVVVPALPRISNHTDFDPLRAHPQVEFT
HHHHHHHHHHCHHHCCCHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCEEEE
YWKSGPVPDADLLILPGSKSVQRDLAWLRDAGWDALIRRHLRYGGKVIGICGGMQMLGRT
EECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCHHHHHHH
LDDPLGLEGAPGSVPGLGLLDFDTTLQPDKTLKNVTGQLALPGAAAVRGYEIHMGDTRGP
CCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCEECCCHHHEEEEEEEECCCCCC
ALETPALMLAADIAREGVRPDGALSADGQILATYVHGLFDAPDACAALLAWAGLDGAARI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
DYPALREASLERLADTFAAHLDLDALYAEFR
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg; Cl- [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; CO2; 7,8-Diaminononanoate [C]

Specific reaction: ATP + CO2 + 7,8-Diaminononanoate <==> ADP + Dethiobiotin + (3) H+ + Phosphate [C]

General reaction: Carboxylation; Heteroatomic ring closure [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA