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The map label for this gene is hemD [H]

Identifier: 116690444

GI number: 116690444

Start: 2688435

End: 2690405

Strand: Reverse

Name: hemD [H]

Synonym: Bcen2424_2424

Alternate gene names: 116690444

Gene position: 2690405-2688435 (Counterclockwise)

Preceding gene: 116690445

Following gene: 116690443

Centisome position: 77.22

GC content: 70.83

Gene sequence:

>1971_bases
ATGGCGGGCGGCGCGCGCGCGTTCACCGCTGTCCTGACGCGTCCGGACGGGCAGTCCGACGCGCTTGCGGCGCAACTGGC
CGATGCCGGCTGCGACGTGCTCGAATTCCCGCTGATCGATATCGCGCCGGTCGACGATCCGGCGCCGCTCGACGCCGCGT
TCGCGGCGCTGGCCGACTACGCGCTGGTGATCTTCGTGTCGCCGAACGCGATCGACCGCGCGCTCGCGCGGTACGACGCG
ATCTGGCCGAATGCGCTGCCGGTCGGCGTGGTCGGGCCCGGCAGCGTCGCGGCGCTCGAGCGCCACGGCATCGCGGCGCC
CGCGCATCGCGTGATCGCGCCGCAGGCGCCGGCCGACGGCGGCGTGCCCCATTACGATTCGGAAAGCCTGTTCGCGAGCA
TCGAAGTCGCGTTCGGCGGCGCGCAGGCGCTCGCGGGCAAGCGCGTGCTGATCGTGCGCGGCGACGGCGGGCGCGAATGG
CTCGCCGAGCGCCTGCGCGAGGCCGGCGCGGATGTCACGCTCGTGGCCGCGTACCGGCGCGTCGTGCCGGAGCCGCGCGT
CGGCGCATGGGAGCGCGTGCACGCGTTGCTCGACGGCCAGCCGCATGGGTGGCTCGTCACGAGCTCGGAAGGCGTGCGCA
ACCTGCACGAGCTTGCGCGGGCCCATCTGAACGAGGCCGAGATCGATGCGCTCAAGCATGCGCCGCTCGTCACGCCGCAC
CCGCGGATCGAGCAGACCGCGCGGGCATTGGGTTTTGATAGGATTACGCTGACCGGCGCGGGCGATGAGCGCATCGTCCG
CGCGTTTCGAACGATGGCCGACGAGGCCGTCCAACCGGCGACAGCCGTACCGGTGACTAAACGCATGACAGATACCAACG
ATTCCAAAAGCGTCGCTTCCCAGCCGGCCCCTGCATCCGCACCGCCGTCCAGCCCCCCTTATCTCGCGCCCGAGCCGCGT
GCGCGCCGCGGCGGCAGCGCGGTGCTGTGGTTCGTCGTCGTCGTGCTCGGCTGCGCGGCGGGTGTCGGCGGCTACGCGCT
GAACCGCAAGATCGACCGGCTCGACGGCACCTTGGTCGCGCGCCAGAAGGCGCTCGACGCGCAAGCGGCCGAAACGCGGC
TGAAGACCGAGCAGGCGCTCGCCAGCACGCACCAGGTCGACACGCAGCTCGCGCAGCTCGACGGCAAGCTTGCCGATGCG
CAGAGCGCGCAGCAGGCGCTGCAGCAGCAATACCAGGACCTGTCGCGCAACCGCGACGCGTGGATGCTCGAGGAGGTCGA
CCAGATGCTGTCGAGCGCGAGCCAGCAGCTTCAACTGACCGGCAACACGCAACTCGCGCTGATCGCGCTGCAGAACGCCG
ACGCGCGTCTCGCGACGTCGCAGAGCGCGCAGGCCGTCACGGTGCGCAAGGCGCTCGCGCTGGACATCGAGAAGCTGAAG
GCTGCGCCCACCGCCGATCTCACCGGCCTCGCGATCAAGCTCGACGACGCGATCGCCAGGATCGACGCGCTGCCGCTGTC
CGGCGAGGCGATCGTGCCGCACGCGGCGCCGAAGGCCGCGCCGGCCGACACCGCGTCGTCGGTCGCCGCCGGCGAGCCGC
GCTGGAAGGTGTGGTGGCACGACTTCTCGGCCGGCCTCGGCCAGCAACTGAAGGGCCTCGTGCAGGTGCGCCGGATCGAC
AACGCGGACGCGATGCTCGCGTCGCCCGACCAGGGCTACTTCGTGCGCGAGAACGTGAAGCTGCGCCTGCTGACCGCACG
GCTGTCGCTGCTGGCGCGCAACGACAGCGCGATGAAGGCCGACCTGCACGCCGCACAGGCGTCGCTCGGGAAGTATTTCG
ATCAGGCGTCGAAGGACACGCAGACCGTCGAGGATCTGCTCAAGCAGGTTGACGGCGCATCGCTGACGGTCGCGGTGCCG
AACCTGAACACGAGCCTGAACGCCGTTCAGCAGTTCAAGAGCCGGGGGTAA

Upstream 100 bases:

>100_bases
TCGGCCGCGCGGTGTCCGACGAACTCGAGGCGCAAGGCGCGCTCGACATCGTCCAGGCGCTGCTCGCGAGCTCGCAGGCC
GCGGGCAAGGGCGACGCCTG

Downstream 100 bases:

>100_bases
CGATGACGCTTCGAGGAATCGTCTGGCTCGCGGTCCTGTTCGCGATCGCCGCGGCGCTCGCCACCGTCGGCCGTTTCGAC
GCGGGGCAGGTGCTGCTCGT

Product: bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MAGGARAFTAVLTRPDGQSDALAAQLADAGCDVLEFPLIDIAPVDDPAPLDAAFAALADYALVIFVSPNAIDRALARYDA
IWPNALPVGVVGPGSVAALERHGIAAPAHRVIAPQAPADGGVPHYDSESLFASIEVAFGGAQALAGKRVLIVRGDGGREW
LAERLREAGADVTLVAAYRRVVPEPRVGAWERVHALLDGQPHGWLVTSSEGVRNLHELARAHLNEAEIDALKHAPLVTPH
PRIEQTARALGFDRITLTGAGDERIVRAFRTMADEAVQPATAVPVTKRMTDTNDSKSVASQPAPASAPPSSPPYLAPEPR
ARRGGSAVLWFVVVVLGCAAGVGGYALNRKIDRLDGTLVARQKALDAQAAETRLKTEQALASTHQVDTQLAQLDGKLADA
QSAQQALQQQYQDLSRNRDAWMLEEVDQMLSSASQQLQLTGNTQLALIALQNADARLATSQSAQAVTVRKALALDIEKLK
AAPTADLTGLAIKLDDAIARIDALPLSGEAIVPHAAPKAAPADTASSVAAGEPRWKVWWHDFSAGLGQQLKGLVQVRRID
NADAMLASPDQGYFVRENVKLRLLTARLSLLARNDSAMKADLHAAQASLGKYFDQASKDTQTVEDLLKQVDGASLTVAVP
NLNTSLNAVQQFKSRG

Sequences:

>Translated_656_residues
MAGGARAFTAVLTRPDGQSDALAAQLADAGCDVLEFPLIDIAPVDDPAPLDAAFAALADYALVIFVSPNAIDRALARYDA
IWPNALPVGVVGPGSVAALERHGIAAPAHRVIAPQAPADGGVPHYDSESLFASIEVAFGGAQALAGKRVLIVRGDGGREW
LAERLREAGADVTLVAAYRRVVPEPRVGAWERVHALLDGQPHGWLVTSSEGVRNLHELARAHLNEAEIDALKHAPLVTPH
PRIEQTARALGFDRITLTGAGDERIVRAFRTMADEAVQPATAVPVTKRMTDTNDSKSVASQPAPASAPPSSPPYLAPEPR
ARRGGSAVLWFVVVVLGCAAGVGGYALNRKIDRLDGTLVARQKALDAQAAETRLKTEQALASTHQVDTQLAQLDGKLADA
QSAQQALQQQYQDLSRNRDAWMLEEVDQMLSSASQQLQLTGNTQLALIALQNADARLATSQSAQAVTVRKALALDIEKLK
AAPTADLTGLAIKLDDAIARIDALPLSGEAIVPHAAPKAAPADTASSVAAGEPRWKVWWHDFSAGLGQQLKGLVQVRRID
NADAMLASPDQGYFVRENVKLRLLTARLSLLARNDSAMKADLHAAQASLGKYFDQASKDTQTVEDLLKQVDGASLTVAVP
NLNTSLNAVQQFKSRG
>Mature_655_residues
AGGARAFTAVLTRPDGQSDALAAQLADAGCDVLEFPLIDIAPVDDPAPLDAAFAALADYALVIFVSPNAIDRALARYDAI
WPNALPVGVVGPGSVAALERHGIAAPAHRVIAPQAPADGGVPHYDSESLFASIEVAFGGAQALAGKRVLIVRGDGGREWL
AERLREAGADVTLVAAYRRVVPEPRVGAWERVHALLDGQPHGWLVTSSEGVRNLHELARAHLNEAEIDALKHAPLVTPHP
RIEQTARALGFDRITLTGAGDERIVRAFRTMADEAVQPATAVPVTKRMTDTNDSKSVASQPAPASAPPSSPPYLAPEPRA
RRGGSAVLWFVVVVLGCAAGVGGYALNRKIDRLDGTLVARQKALDAQAAETRLKTEQALASTHQVDTQLAQLDGKLADAQ
SAQQALQQQYQDLSRNRDAWMLEEVDQMLSSASQQLQLTGNTQLALIALQNADARLATSQSAQAVTVRKALALDIEKLKA
APTADLTGLAIKLDDAIARIDALPLSGEAIVPHAAPKAAPADTASSVAAGEPRWKVWWHDFSAGLGQQLKGLVQVRRIDN
ADAMLASPDQGYFVRENVKLRLLTARLSLLARNDSAMKADLHAAQASLGKYFDQASKDTQTVEDLLKQVDGASLTVAVPN
LNTSLNAVQQFKSRG

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790235, Length=366, Percent_Identity=24.0437158469945, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790236, Length=277, Percent_Identity=27.4368231046931, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 69529; Mature: 69398

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGARAFTAVLTRPDGQSDALAAQLADAGCDVLEFPLIDIAPVDDPAPLDAAFAALADY
CCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHCC
ALVIFVSPNAIDRALARYDAIWPNALPVGVVGPGSVAALERHGIAAPAHRVIAPQAPADG
EEEEEECCCHHHHHHHHHHCCCCCCCEECEECCCCHHHHHHCCCCCCCHHEECCCCCCCC
GVPHYDSESLFASIEVAFGGAQALAGKRVLIVRGDGGREWLAERLREAGADVTLVAAYRR
CCCCCCCCCCEEEEEEEECCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHH
VVPEPRVGAWERVHALLDGQPHGWLVTSSEGVRNLHELARAHLNEAEIDALKHAPLVTPH
HCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
PRIEQTARALGFDRITLTGAGDERIVRAFRTMADEAVQPATAVPVTKRMTDTNDSKSVAS
CCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHC
QPAPASAPPSSPPYLAPEPRARRGGSAVLWFVVVVLGCAAGVGGYALNRKIDRLDGTLVA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHCCHHHH
RQKALDAQAAETRLKTEQALASTHQVDTQLAQLDGKLADAQSAQQALQQQYQDLSRNRDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
WMLEEVDQMLSSASQQLQLTGNTQLALIALQNADARLATSQSAQAVTVRKALALDIEKLK
HHHHHHHHHHHHHHHEEEEECCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHC
AAPTADLTGLAIKLDDAIARIDALPLSGEAIVPHAAPKAAPADTASSVAAGEPRWKVWWH
CCCCCCCCEEEEEEHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHCCCCCCEEEEEE
DFSAGLGQQLKGLVQVRRIDNADAMLASPDQGYFVRENVKLRLLTARLSLLARNDSAMKA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCHHHH
DLHAAQASLGKYFDQASKDTQTVEDLLKQVDGASLTVAVPNLNTSLNAVQQFKSRG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AGGARAFTAVLTRPDGQSDALAAQLADAGCDVLEFPLIDIAPVDDPAPLDAAFAALADY
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHCC
ALVIFVSPNAIDRALARYDAIWPNALPVGVVGPGSVAALERHGIAAPAHRVIAPQAPADG
EEEEEECCCHHHHHHHHHHCCCCCCCEECEECCCCHHHHHHCCCCCCCHHEECCCCCCCC
GVPHYDSESLFASIEVAFGGAQALAGKRVLIVRGDGGREWLAERLREAGADVTLVAAYRR
CCCCCCCCCCEEEEEEEECCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHH
VVPEPRVGAWERVHALLDGQPHGWLVTSSEGVRNLHELARAHLNEAEIDALKHAPLVTPH
HCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
PRIEQTARALGFDRITLTGAGDERIVRAFRTMADEAVQPATAVPVTKRMTDTNDSKSVAS
CCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHC
QPAPASAPPSSPPYLAPEPRARRGGSAVLWFVVVVLGCAAGVGGYALNRKIDRLDGTLVA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHCCHHHH
RQKALDAQAAETRLKTEQALASTHQVDTQLAQLDGKLADAQSAQQALQQQYQDLSRNRDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
WMLEEVDQMLSSASQQLQLTGNTQLALIALQNADARLATSQSAQAVTVRKALALDIEKLK
HHHHHHHHHHHHHHHEEEEECCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHC
AAPTADLTGLAIKLDDAIARIDALPLSGEAIVPHAAPKAAPADTASSVAAGEPRWKVWWH
CCCCCCCCEEEEEEHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHCCCCCCEEEEEE
DFSAGLGQQLKGLVQVRRIDNADAMLASPDQGYFVRENVKLRLLTARLSLLARNDSAMKA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCHHHH
DLHAAQASLGKYFDQASKDTQTVEDLLKQVDGASLTVAVPNLNTSLNAVQQFKSRG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8159168; 10984043 [H]