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The map label for this gene is nadE [H]

Identifier: 116690432

GI number: 116690432

Start: 2673036

End: 2674754

Strand: Reverse

Name: nadE [H]

Synonym: Bcen2424_2412

Alternate gene names: 116690432

Gene position: 2674754-2673036 (Counterclockwise)

Preceding gene: 116690434

Following gene: 116690431

Centisome position: 76.77

GC content: 68.0

Gene sequence:

>1719_bases
ATGAAGACCCGACTCGCTCTCGCCCAGATCAACGTCACCGTCGGCGATTTCGCCGGCAACGTCGCGCGGATCGTCGCGGC
CGCACGCGCCGCGCACAACGATGGTGCGCAACTGATGGTCGCACCCGAACTCGCGCTGTCCGGCTATCCACCCGAAGACC
TGCTGCTGCGGCCCGCGTTCTACGCGGCGGCGGCCGCCGCGCTCGATGCGCTCGCCGACGCGCTGAAAGCGTTCGACGGG
CTCGCGGTGCTGGTCGGCCACCCGTTGCGCGGTCCGGGCAGCGGGGCGGGCGACGATGCGCGCGCGTCAGCCGTCGATGG
TAATGCAAACCGCCCGATCGAGCGCGGCGTGCCGCCTACCGACACCTTCAACGCGGTATCGCTGATCGTCGGCGGCGAGA
TCGTCGGCACCTACCGCAAGCAGGATCTGCCGAACGCCGAGGTGTTCGACGAGAAACGCTATTTCGCGACCGACACCGAG
CCGCTCGTGTTCGAGCTGAACGGCGTGAAATACGGCGTGATCATCTGCGAGGACGCGTGGCATGCGTCGGCCGCGCAGAT
CGCGAAGGCGGCCGGCGCGCAGGTGCTGCTGATCCCGAACGGCTCGCCGTATCACCTGAACAAGGAAGCGGTGCGCATCG
ACATCCTGCGCGCGCGGATTCGCGAGACCGGGCTGCCGATGGTGTACGTGAACCTCGTCGGTGGCCAGGACGAACTCGTG
TTCGACGGCGGGTCGTTCGTGCTCGACGGCGAGGGCGGGCTCGTCGCGAAGATGCCGCAGTTCGACGAAGGGCACGCGAT
CGTCGAATTCGACGGCGCGCGGCCACTGCCCGGCGCGATCGCGCCCGAGCTGTCGACCGACGCGCAGGTGTACCGCGCGC
TCGTGACCGGCGTGCGCGACTACATCGGCAAGAACGGTTTTCCCGGCGTGCTGATCGGGCTGTCGGGCGGCGTCGATTCG
GCGCTGGTGCTGGCCGTCGCATGTGATGCGCTCGGGCCCGAGCGCGTGCGCGCGGTGATGATGCCGTCGCGCTTCACGGC
CGACATCTCGACGACGGACGCGGCGGAGATGGCGCGGCGCGTCGGCGTACGCTACGACGAGATCGCGATCGCGCCGATGT
TCGATGCGTTCCGCGTCGCGCTCGCGGGCGAATTCGCGGGCCGCGCGGAAGACGCGACGGAGGAGAACATCCAGGCGCGT
ATCCGCGGCACGCTGCTGATGGCGCTGTCGAACAAGTTCGGCTCGATCGTGCTGACGACTGGCAACAAGAGCGAGATGGC
GGTCGGCTATTGCACGTTGTACGGCGACATGGCCGGCGGTTTCGCGGTGATCAAGGACATCGCGAAGACGCTCGTGTACC
GGCTGTGCCGCTATCGCAACGCAACGACCGACTACGGGCTGCGCGACGTGATTCCCGAGCGAATCCTGACGCGCGCGCCG
TCGGCCGAGCTGCGCGAGAACCAGACCGACCAGGACAGCCTGCCGCCATACGACGTGCTCGACGCGATCATGCGGATGTA
CATGGAAGAAGACCGGCCGCTCGCCGAGATTGTCGCGGCCGGCTATGCGCAGGCCGACGTCGAGCGCGTGACGCGGCTCA
TCAAGATCAACGAATACAAGCGCCGCCAGGCGCCGATCGGCATCCGCGTCACGCATCGCGCGTTCGGGCGCGACTGGCGC
TACCCGATCACGTCACGCTTCACCGAACGGCTCGATTGA

Upstream 100 bases:

>100_bases
CGTACCGTTGGATGTTGCCGGATGCGGGCCGCCGTGGCCCGCATCCGGGTCGATTTCGTCAGCCGTTCAAGGCGCGCCGC
CGGGTTGCGCCGATCCCGCC

Downstream 100 bases:

>100_bases
GCCGTGCTGGGCCGGAGGCGGCCCGCCGGCTTACAATCGGGAACGCGATTTTTCATCCAAACGAGCATTGAGGGACAACC
ATCATGAAACGCATCACCGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAAAALDALADALKAFDG
LAVLVGHPLRGPGSGAGDDARASAVDGNANRPIERGVPPTDTFNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDTE
PLVFELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHLNKEAVRIDILRARIRETGLPMVYVNLVGGQDELV
FDGGSFVLDGEGGLVAKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGVLIGLSGGVDS
ALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRVALAGEFAGRAEDATEENIQAR
IRGTLLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNATTDYGLRDVIPERILTRAP
SAELRENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQADVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWR
YPITSRFTERLD

Sequences:

>Translated_572_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAAAALDALADALKAFDG
LAVLVGHPLRGPGSGAGDDARASAVDGNANRPIERGVPPTDTFNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDTE
PLVFELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHLNKEAVRIDILRARIRETGLPMVYVNLVGGQDELV
FDGGSFVLDGEGGLVAKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGVLIGLSGGVDS
ALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRVALAGEFAGRAEDATEENIQAR
IRGTLLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNATTDYGLRDVIPERILTRAP
SAELRENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQADVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWR
YPITSRFTERLD
>Mature_572_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAAAALDALADALKAFDG
LAVLVGHPLRGPGSGAGDDARASAVDGNANRPIERGVPPTDTFNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDTE
PLVFELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHLNKEAVRIDILRARIRETGLPMVYVNLVGGQDELV
FDGGSFVLDGEGGLVAKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGVLIGLSGGVDS
ALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRVALAGEFAGRAEDATEENIQAR
IRGTLLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNATTDYGLRDVIPERILTRAP
SAELRENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQADVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWR
YPITSRFTERLD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61621; Mature: 61621

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHHEECHHH
YAAAAAALDALADALKAFDGLAVLVGHPLRGPGSGAGDDARASAVDGNANRPIERGVPPT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
DTFNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDTEPLVFELNGVKYGVIICEDAW
CCCCCEEEEECCCEEECHHHCCCCCCHHCCCCCEEECCCCCEEEEECCEEEEEEEECCCC
HASAAQIAKAAGAQVLLIPNGSPYHLNKEAVRIDILRARIRETGLPMVYVNLVGGQDELV
CCHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
FDGGSFVLDGEGGLVAKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRD
EECCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YIGKNGFPGVLIGLSGGVDSALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARR
HHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHH
VGVRYDEIAIAPMFDAFRVALAGEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTT
HCCCCHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEE
GNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNATTDYGLRDVIPERILTRAP
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
SAELRENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQADVERVTRLIKINEYK
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRQAPIGIRVTHRAFGRDWRYPITSRFTERLD
HHCCCCEEEEEHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHHEECHHH
YAAAAAALDALADALKAFDGLAVLVGHPLRGPGSGAGDDARASAVDGNANRPIERGVPPT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
DTFNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDTEPLVFELNGVKYGVIICEDAW
CCCCCEEEEECCCEEECHHHCCCCCCHHCCCCCEEECCCCCEEEEECCEEEEEEEECCCC
HASAAQIAKAAGAQVLLIPNGSPYHLNKEAVRIDILRARIRETGLPMVYVNLVGGQDELV
CCHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
FDGGSFVLDGEGGLVAKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRD
EECCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YIGKNGFPGVLIGLSGGVDSALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARR
HHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHH
VGVRYDEIAIAPMFDAFRVALAGEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTT
HCCCCHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEE
GNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNATTDYGLRDVIPERILTRAP
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
SAELRENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQADVERVTRLIKINEYK
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRQAPIGIRVTHRAFGRDWRYPITSRFTERLD
HHCCCCEEEEEHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]