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The map label for this gene is otsA [H]

Identifier: 116690413

GI number: 116690413

Start: 2654922

End: 2656343

Strand: Reverse

Name: otsA [H]

Synonym: Bcen2424_2393

Alternate gene names: 116690413

Gene position: 2656343-2654922 (Counterclockwise)

Preceding gene: 116690414

Following gene: 116690412

Centisome position: 76.25

GC content: 68.5

Gene sequence:

>1422_bases
ATGAGCAGATTGATCGTGGTATCGAATCGTGTCGCCGCCGGCGAGGACACGCGCCCGAGCGCGGGCGGCCTGGCCGTCGG
CGTGATGGACGCGCTGAAGGAAACGGGCGGCGTCTGGTTCGGCTGGAACGGCGAGATCGTCGGGGCGCCGGATGCCGAGC
CCGCGATCCAGCGGGACGGCAACGTGACGTATGCGACGGTCGGGTTGACCCGGCGCGACTACGACCAGTACTACCGCGGC
TTCTCGAACGCGACGCTGTGGCCGGTGTTCCACTACCGCGGCGATCTCGCGCGCTTCGATCGGCAGGAGTACGCCGGTTA
TCTGCGCGTGAACGCGATGCTCGCGAAGCAGCTCGCCGCGCTGCTGCGTCCGGACGACCTGATCTGGGTGCACGACTACC
ACCTGCTGCCGTTCGCGCACTACCTGCGCGAACTCGGCGTGAAGAACCCGATCGGCTTCTTCCTGCACATCCCGTTTCCG
TCGCCCGACATGCTGCGCCTCGTGCCGCCGCACGAAGAACTCGTGAAGTTCATGTGCGCGTACGACGTCGCGGGCTTCCA
GACCGAGGCCGACAAACAGGCGTTCACCGACTACATCGAGCGGCGCGGCATCGGCGCGGCGAGCGAGGACGGGATGCTGC
ACGCGCATGGCCGCGTCGTGAAGGTCGCGGCCTATCCGATCGGCGTGCATCCCGACGCGATCGCGCAGGCGGCCGTCCAG
TACGGCACGCGCAAGCCGGTGAAGATGCTGCGCGAGGCGCTGGGCGACCGCAAGCTGGTGATGAGCGTCGACCGCCTCGA
TTACTCGAAGGGGCTCGTCGAGCGCTTCCAGTCGTTCGAGCGGATGCTCGCGAACGCGCCGGGCTGGCAGGGGCGCGTGT
CGCTGCTGCAAATCGCGCCGCCGACGCGTTCCGACGTGCAGACCTATCAGCAGATCCGCGAAACGCTCGAAGGCGAGGCC
GGCCGCATCAACGGCCGTTTCTCGCAGCTCGACTGGACGCCGATCCAGTACCTGAACCGCAAGTACGAGCGCAACCTGCT
GATGGCGTTCTTCCGGATATCGCAGGTCGGCTACGTGACGCCGCTGCGCGACGGGATGAACCTCGTCGCGAAGGAATACG
TCGCATCGCAGGACCCGGCCGATCCGGGCGTGCTGGTGCTGTCGGAGTTCGCGGGCGCGGCGGCCGAGCTGACTGGCGCG
CTGCTCGTCAATCCGTACGACCTGTCGCAGATGGCCGAGGCGCTCGAACGCGCGCTGTCGATGCCGCTCGCGGAGCGGCA
GGCGCGTCACGAGCAGAATCTCGCGCGGCTGCGCGAGAACGACCTGTCGGTGTGGCGCGATACGTTCGTCGCCGACCTGC
GCAGCGTCGCGGCGGCGGCGTCGGTCACGCGGCGTGCGGGCCGGCGCGTCGCGCATGCGTGA

Upstream 100 bases:

>100_bases
AATTCTCGGAACTGAATCGCCGCGCGGCCGCCCTCGGGCCGCCGCGCCCGCTTTGGCGTGCGTCGCGCGCCAGCCAATCG
AATCGAGGAGGGGAAGTCGC

Downstream 100 bases:

>100_bases
GTCCGCGCCGGGCGCCGGTAGCGACGACGAAGCGGCCGACGATACCGCCGGCCGTTTCTTCGTCGTGACGGGCGGCCCGG
GTTCGGGCAAGAGCACGTTG

Product: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRLIVVSNRVAAGEDTRPSAGGLAVGVMDALKETGGVWFGWNGEIVGAPDAEPAIQRDGNVTYATVGLTRRDYDQYYRG
FSNATLWPVFHYRGDLARFDRQEYAGYLRVNAMLAKQLAALLRPDDLIWVHDYHLLPFAHYLRELGVKNPIGFFLHIPFP
SPDMLRLVPPHEELVKFMCAYDVAGFQTEADKQAFTDYIERRGIGAASEDGMLHAHGRVVKVAAYPIGVHPDAIAQAAVQ
YGTRKPVKMLREALGDRKLVMSVDRLDYSKGLVERFQSFERMLANAPGWQGRVSLLQIAPPTRSDVQTYQQIRETLEGEA
GRINGRFSQLDWTPIQYLNRKYERNLLMAFFRISQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSEFAGAAAELTGA
LLVNPYDLSQMAEALERALSMPLAERQARHEQNLARLRENDLSVWRDTFVADLRSVAAAASVTRRAGRRVAHA

Sequences:

>Translated_473_residues
MSRLIVVSNRVAAGEDTRPSAGGLAVGVMDALKETGGVWFGWNGEIVGAPDAEPAIQRDGNVTYATVGLTRRDYDQYYRG
FSNATLWPVFHYRGDLARFDRQEYAGYLRVNAMLAKQLAALLRPDDLIWVHDYHLLPFAHYLRELGVKNPIGFFLHIPFP
SPDMLRLVPPHEELVKFMCAYDVAGFQTEADKQAFTDYIERRGIGAASEDGMLHAHGRVVKVAAYPIGVHPDAIAQAAVQ
YGTRKPVKMLREALGDRKLVMSVDRLDYSKGLVERFQSFERMLANAPGWQGRVSLLQIAPPTRSDVQTYQQIRETLEGEA
GRINGRFSQLDWTPIQYLNRKYERNLLMAFFRISQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSEFAGAAAELTGA
LLVNPYDLSQMAEALERALSMPLAERQARHEQNLARLRENDLSVWRDTFVADLRSVAAAASVTRRAGRRVAHA
>Mature_472_residues
SRLIVVSNRVAAGEDTRPSAGGLAVGVMDALKETGGVWFGWNGEIVGAPDAEPAIQRDGNVTYATVGLTRRDYDQYYRGF
SNATLWPVFHYRGDLARFDRQEYAGYLRVNAMLAKQLAALLRPDDLIWVHDYHLLPFAHYLRELGVKNPIGFFLHIPFPS
PDMLRLVPPHEELVKFMCAYDVAGFQTEADKQAFTDYIERRGIGAASEDGMLHAHGRVVKVAAYPIGVHPDAIAQAAVQY
GTRKPVKMLREALGDRKLVMSVDRLDYSKGLVERFQSFERMLANAPGWQGRVSLLQIAPPTRSDVQTYQQIRETLEGEAG
RINGRFSQLDWTPIQYLNRKYERNLLMAFFRISQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSEFAGAAAELTGAL
LVNPYDLSQMAEALERALSMPLAERQARHEQNLARLRENDLSVWRDTFVADLRSVAAAASVTRRAGRRVAHA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=466, Percent_Identity=52.1459227467811, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI115533558, Length=357, Percent_Identity=30.2521008403361, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI115533560, Length=357, Percent_Identity=30.2521008403361, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71987012, Length=435, Percent_Identity=28.5057471264368, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319602, Length=468, Percent_Identity=33.5470085470085, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320279, Length=417, Percent_Identity=27.0983213429257, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6323537, Length=385, Percent_Identity=27.7922077922078, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323917, Length=373, Percent_Identity=27.3458445040214, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI19920676, Length=497, Percent_Identity=36.8209255533199, Blast_Score=289, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52865; Mature: 52734

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVAAGEDTRPSAGGLAVGVMDALKETGGVWFGWNGEIVGAPDAEPAIQRDG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCHHCCC
NVTYATVGLTRRDYDQYYRGFSNATLWPVFHYRGDLARFDRQEYAGYLRVNAMLAKQLAA
CEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHH
LLRPDDLIWVHDYHLLPFAHYLRELGVKNPIGFFLHIPFPSPDMLRLVPPHEELVKFMCA
HHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHH
YDVAGFQTEADKQAFTDYIERRGIGAASEDGMLHAHGRVVKVAAYPIGVHPDAIAQAAVQ
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHHHHH
YGTRKPVKMLREALGDRKLVMSVDRLDYSKGLVERFQSFERMLANAPGWQGRVSLLQIAP
HCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PTRSDVQTYQQIRETLEGEAGRINGRFSQLDWTPIQYLNRKYERNLLMAFFRISQVGYVT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLRDGMNLVAKEYVASQDPADPGVLVLSEFAGAAAELTGALLVNPYDLSQMAEALERALS
CCHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHCCEEEECCCCHHHHHHHHHHHHC
MPLAERQARHEQNLARLRENDLSVWRDTFVADLRSVAAAASVTRRAGRRVAHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLIVVSNRVAAGEDTRPSAGGLAVGVMDALKETGGVWFGWNGEIVGAPDAEPAIQRDG
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCHHCCC
NVTYATVGLTRRDYDQYYRGFSNATLWPVFHYRGDLARFDRQEYAGYLRVNAMLAKQLAA
CEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHH
LLRPDDLIWVHDYHLLPFAHYLRELGVKNPIGFFLHIPFPSPDMLRLVPPHEELVKFMCA
HHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHH
YDVAGFQTEADKQAFTDYIERRGIGAASEDGMLHAHGRVVKVAAYPIGVHPDAIAQAAVQ
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHHHHH
YGTRKPVKMLREALGDRKLVMSVDRLDYSKGLVERFQSFERMLANAPGWQGRVSLLQIAP
HCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PTRSDVQTYQQIRETLEGEAGRINGRFSQLDWTPIQYLNRKYERNLLMAFFRISQVGYVT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLRDGMNLVAKEYVASQDPADPGVLVLSEFAGAAAELTGALLVNPYDLSQMAEALERALS
CCHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHCCEEEECCCCHHHHHHHHHHHHC
MPLAERQARHEQNLARLRENDLSVWRDTFVADLRSVAAAASVTRRAGRRVAHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA