Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 116690280

GI number: 116690280

Start: 2514465

End: 2516009

Strand: Reverse

Name: leuA [H]

Synonym: Bcen2424_2259

Alternate gene names: 116690280

Gene position: 2516009-2514465 (Counterclockwise)

Preceding gene: 116690281

Following gene: 116690276

Centisome position: 72.22

GC content: 63.82

Gene sequence:

>1545_bases
ATGACAGACAAGCTGATCATTTTCGATACGACGTTGCGTGACGGCGAGCAATCGCCCGGCGCGTCGATGACGAAGGAAGA
GAAAATCCGCATCGCGAAGCACCTCGAGCGGATGAAGGTCGACGTGATCGAGGCCGGTTTCGCCGCCAGCTCGAACGGCG
ATTTCGACGCGATCCACACGATCGCCGGTCTCGTGAAGGACAGCACGATCTGTTCGCTGGCGCGGGCCAACGACAAGGAC
ATCCAGCGCGCGGCGGACGCGCTCAAGCCGGCCAACAGCGCGCGGATCCATACGTTCATCGCGACGTCGCCGCTGCACAT
GGAGAAGAAGCTGCGGATGACGCCGGACCAGGTGTTCGAGCAGGCGCGTCTCGCGGTGCGTTTCGCGCGCAAGTTCACCG
ACAACGTCGAATTCTCGCCGGAAGACGGCAGCCGTTCGGATCTCGATTTCCTGTGCCGTGTGCTGGAAGCGGTGATCGCC
GAGGGCGCGACGACGATCAACATCGCCGATACGGTCGGCTACGGCGTGCCGGAACTCTACGGCAACCTCGTGAAGACGCT
GCGCGAGCGCATTCCGAACTCGGACAAGGCGATCTTCTCCGTGCATTGCCACAATGACCTCGGGATGGCGGTGGCGAACT
CGCTCGCCGGCGTGAAGATCGGCGGTGCGCGGCAGATCGAATGCACGATCAACGGTCTCGGCGAGCGCGCGGGCAACACG
TCGCTCGAGGAAATCGTGATGGCCGTGAAGACCCGCAAGGACTACTTCGGTCTCGACGTCGGCATCGACACGACGCAGAT
CGTGCCGACGTCGAAGCTCGTGTCGCAGATTACCGGCTTCGTCGTGCAGCCGAACAAGGCGGTGGTCGGCGCCAACGCAT
TCGCGCACGCATCGGGCATTCACCAGGACGGCGTGCTGAAGGCGCGCGACACCTACGAGATCATGCGTGCGGAAGACGTG
GGCTGGACCGCGAACAAGATCGTGCTCGGCAAGCTGTCGGGCCGCAACGCGTTCAAGCAGCGTCTGCAGGAGCTGGGCGT
GTCGCTCGACAGCGAAGCCGAACTGAATGCCGCGTTCATGCGCTTCAAGGATCTGGCCGACCGCAAGGCCGAGATCTTCG
ACGAGGACATCATCGCGATCGTGTCCGAGGAATCGGCGCTCGCGCAGGAACAGGAGCACTTCAAGTTCGTGTCGCTGTCG
CAGCGTTCGGAAACGGGCGAGCAGCCGCAGGCGAAGGTCGTGTTCGCGGTCGAAGGCAAGGAAGTGACCGGCGAGGCGCG
CGGCAACGGCCCGGTCGACGCGACGTTCAATGCGATCGAGGGTGAAGTCGGCAGCGGGTCCGAGCTGCTGCTGTACTCGG
TCAACGCGATCACGACCGGCACGCAGGCGCAGGGCGAAGTGACCGTCCGGCTGTCGAAGAGCGGGCGGATCGTCAACGGC
GTGGGCACCGATCCGGATATCGTCGCCGCATCCGCGAAGGCGTACATTTCCGCGCTGAACAAGCTGCACTCGAAGGACGA
CAAGCTCAACCCGCAGCGCGCGTAA

Upstream 100 bases:

>100_bases
CTCGCCCTCCGTCTCAAGCCTCGTCTGTTGTTGAGCGTCGCCAGCGGTGGCGCGATCTCGTTACGTTTGACTTCCGTATA
AAAGAGCCCTGGAGCCCCCC

Downstream 100 bases:

>100_bases
GCGCCGCGCGTTGCGCGGCACCGAGACGCCCCGTGCGGCCCTGCGCCGCCGGGGCGTTTTTTCATTGGCCGCTCATTCCG
CGGCCGGCGCGCGGAACAGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKD
IQRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNT
SLEEIVMAVKTRKDYFGLDVGIDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFMRFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLS
QRSETGEQPQAKVVFAVEGKEVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYISALNKLHSKDDKLNPQRA

Sequences:

>Translated_514_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKD
IQRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNT
SLEEIVMAVKTRKDYFGLDVGIDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFMRFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLS
QRSETGEQPQAKVVFAVEGKEVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYISALNKLHSKDDKLNPQRA
>Mature_513_residues
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKDI
QRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIAE
GATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNTS
LEEIVMAVKTRKDYFGLDVGIDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFMRFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLSQ
RSETGEQPQAKVVFAVEGKEVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYISALNKLHSKDDKLNPQRA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=49.5049504950495, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=25.3676470588235, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=552, Percent_Identity=25.3623188405797, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=29.1005291005291, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=28.8359788359788, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55507; Mature: 55376

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLVKDSTICSLARANDKDIQRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCHHHHHH
QARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFGLDVGIDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCEECCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFM
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLSQRSETGEQPQAKVVFAVEGK
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEEEECC
EVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCCCEEHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNKLHSKDDKLNPQRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLVKDSTICSLARANDKDIQRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCHHHHHH
QARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFGLDVGIDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCEECCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFM
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLSQRSETGEQPQAKVVFAVEGK
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEEEECC
EVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCCCEEHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNKLHSKDDKLNPQRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA