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The map label for this gene is fumB [H]

Identifier: 116690215

GI number: 116690215

Start: 2446064

End: 2447587

Strand: Direct

Name: fumB [H]

Synonym: Bcen2424_2194

Alternate gene names: 116690215

Gene position: 2446064-2447587 (Clockwise)

Preceding gene: 116690214

Following gene: 116690216

Centisome position: 70.21

GC content: 66.4

Gene sequence:

>1524_bases
ATGACCGTCATCAAACAGGAAGACCTGATCCAGAGCATCGCGGATTCGCTGCAGTACATCAGCTACTACCACCCGCTCGA
CTACATCCAGGCCCTCGGCCGCGCGTACGAGCTGGAAGAGAGCCCGGCGGCGAAGGACGCGATCGAGCAGATCCTCACGA
ACAGCCGCATGTGCGCGGAAGGCAAACGCCCGATCTGCCAGGACACCGGCATCGTGACGATCTTCGTGAAGGTCGGCATG
GACGTGCGCTGGGACGGCGCGACGATGGGCCTCACCGACATGATCAACGAAGGCGTGCGCCGCGGTTACACGCACCCGGA
CAACGTGCTGCGCGCGTCGATCGTCAATCCGCCGGAAGGCGCCCGCAAGAACACGAAAGACAACACGCCGGCCGTGATCC
ACTACGAGATCGTGCCGGGCGACAAGGTCGACGTGCAGGTCGCGGCGAAGGGCGGCGGCTCGGAGAACAAGTCGAAGTTC
GTGATGCTGAACCCGTCCGATTCGATCGTCGACTGGGTGCTGAAGACGGTCCCGACGATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTCGGGATCGGCATCGGCGGCACCGCCGAGAAGGCGATGCTGATGGCGAAGGAATCGCTGATGGAGCCGATCG
ACATCCAGGAAATCATCGCGCGCGGCCCGAAGGACTGGGTCGAGGAACTGCGCGTGGAACTGCACGAGAAGGTCAACGCG
CTCGGCATCGGCGCGCAGGGCCTCGGCGGCCTCGCGACCGTGCTGGACGTGAAGATCATGGCGGCGCCGACGCACGCGGC
GTCGAAGCCGGTCGCGCTGATCCCGAACTGCGCGGCCACGCGCCACGCGCACTTCGTGCTCGACGGGTCGGGCCCGGCCA
AATTGGAAGCGCCGTCGCTCGACGCCTGGCCGAAGGTGCAGTGGGAACCGAACACGGAAACCAGCAAGCGCGTGGACCTG
AACACGCTGACGCCGGAAGAAGTCGCGAGCTGGACGCCGGGCCAGACGCTGCTGCTGTCGGGCAAGATGCTCACCGGCCG
CGACGCCGCGCACAAGCGCATCGCCGACATGCTCGCGAAGGGCGAGAAGCTGCCGGTCGATTTCACGAACCGCGTGATCT
ACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAAGTGGTCGGCCCGGCCGGCCCGACGACGGCCACGCGCATGGACAAG
TTCACCGAGACGATGCTCGCTCAGACGGGTCTGATCTCGATGGTCGGCAAGGCCGAGCGCGGCCCGGTCGCGATCGACGC
GATCAAGAAGCACAAGGCGGCCTACCTGATGGCGGTCGGCGGCGCGGCCTACCTCGTATCGAAGGCGATCCGCGGCGCGA
AGGTGCTCGCGTTCGAAGACCTCGGCATGGAAGCGATCTACGAGTTCGACGTGCAGGACATGCCGGTGACGGTCGCCGTC
GATTCGTCGGGCACGTCGGTGCACCAGACGGGTCCGAAGGAATGGCAGGCGAAGATCGGCAAGATCCCGGTCGCCACCGC
TTAA

Upstream 100 bases:

>100_bases
ACCCGGCCCATTTCCACGAGGCGCACGCGCCCGCGGCGTCCCGACCGGCGGCCTGAACCGTCGGCAAACCCTTCCCCACT
GTCCCCACAGAGTCTCAGCC

Downstream 100 bases:

>100_bases
GCGCGATCGAACGAAAGGCCGGACGGGAGTCCGGCCTTTTTACTTCTCATTCTCATTATTGTGCGCAGCCGGTTATTCAA
GGCTGATTCTCATTACACGC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIEQILTNSRMCAEGKRPICQDTGIVTIFVKVGM
DVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVQWEPNTETSKRVDL
NTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA

Sequences:

>Translated_507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIEQILTNSRMCAEGKRPICQDTGIVTIFVKVGM
DVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVQWEPNTETSKRVDL
NTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA
>Mature_506_residues
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIEQILTNSRMCAEGKRPICQDTGIVTIFVKVGMD
VRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEKVNAL
GIGAQGLGGLATVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSLDAWPKVQWEPNTETSKRVDLN
TLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKF
TETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVTVAVD
SSGTSVHQTGPKEWQAKIGKIPVATA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=457, Percent_Identity=33.2603938730853, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1790564, Length=456, Percent_Identity=31.7982456140351, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789442, Length=283, Percent_Identity=28.2685512367491, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.3743016759777, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54495; Mature: 54364

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIEQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHC
GKRPICQDTGIVTIFVKVGMDVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEECCCCC
DAWPKVQWEPNTETSKRVDLNTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCEEECCCCCCCCEEECCCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVTVAV
CCEEHHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEESPAAKDAIEQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHC
GKRPICQDTGIVTIFVKVGMDVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVALIPNCAATRHAHFVLDGSGPAKLEAPSL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEECCCCC
DAWPKVQWEPNTETSKRVDLNTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCEEECCCCCCCCEEECCCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVTVAV
CCEEHHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]