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The map label for this gene is trmJ [H]

Identifier: 116690105

GI number: 116690105

Start: 2313787

End: 2314656

Strand: Reverse

Name: trmJ [H]

Synonym: Bcen2424_2084

Alternate gene names: 116690105

Gene position: 2314656-2313787 (Counterclockwise)

Preceding gene: 116690108

Following gene: 116690104

Centisome position: 66.44

GC content: 69.77

Gene sequence:

>870_bases
ATGGCGATACCATACGGCCATTCTGATGAACCGAGTCGTATCGTAGTGGAAACTCCGCAGAACACCGCCGCGCCGTCCGA
ATCGGGCGCGGCCTCGTCCCCGCCGCCGTCCGGCGGCTTCACGTCCACCCGCTTCGTGCTCGTCGAGCCGAGCCATCCCG
GCAACGTCGGGGCGGCTGCCCGCGCGCTGAAGACGATGGGCTTTTCGCGCCTCGTGCTGGTCGCGCCGCGCGTGCCGCAC
GTGCAGAGCGACCCCGAGGCGATCGCGATGGCCAGCGGTGCGGACGACGTCCTCGCGTCCGCGCATGTCGTGCCGACGCT
CGGCGAAGCGCTGTCCGGCGTTCACTGGTCGATCGCGCTGACCGCGCGCACGCGCGAATACGGGCCGCCGCGCCTCGCGC
CGCGCGCGGCTGCCACACAGGCGAGCGCGCAGGTCGGCACGGGCGACATCGCGCTCGTGTTCGGCAACGAGCGCACGGGT
CTCGCGAACGAGCATGTCGAGCAGTGCAGCGCGCTCGCGCACATTCCGGCCAACCCGGCCTACAGTTCGCTGAATCTCGC
GCAGGCCGTGCAGGTGCTCGCATACGAGTTGCGCGTCGCGTTTCTCGAGCAGGCGAGCGAGCCGTCGGCACAGCCGCAGG
CCGAAGCCGGCACGCTCGCGCAGAGCGACGAGATCGAGCGGATGTACGTGCACCTCGAGAACGCGTTGATCGCGCTCGAC
TTCCTCGATCCGCGCAATCCGAAGAAGCTGATGCCGCGGCTGCGGCGCCTGTTCGCGCGCACGGGCCTCGAGCGCGAGGA
GGTCAACATCCTGCGCGGCATCGCGAAGCACATCCTGCTGAAGTCGGGCAAACCAGGCGGCGACGCGTAA

Upstream 100 bases:

>100_bases
CGCGTAAAGGCATGATTTTACCCGATTTTCGGCGGTTTCAGGCGACCGGCATGCGGGGAAACCCCCACTGGCCATCCGGA
CGAGTGGGTTTGCCGGGGCG

Downstream 100 bases:

>100_bases
GCGGCCGGCGCTCCGATTGCCGGGGGCGGGGGAGTTCGCTCGATGCGGCGTGCGTTCGGCCGGGCCATGTGTCGACCGAC
CGCACGCGTCGAACCTGCTG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAIPYGHSDEPSRIVVETPQNTAAPSESGAASSPPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAIAMASGADDVLASAHVVPTLGEALSGVHWSIALTARTREYGPPRLAPRAAATQASAQVGTGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQASEPSAQPQAEAGTLAQSDEIERMYVHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSGKPGGDA

Sequences:

>Translated_289_residues
MAIPYGHSDEPSRIVVETPQNTAAPSESGAASSPPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAIAMASGADDVLASAHVVPTLGEALSGVHWSIALTARTREYGPPRLAPRAAATQASAQVGTGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQASEPSAQPQAEAGTLAQSDEIERMYVHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSGKPGGDA
>Mature_288_residues
AIPYGHSDEPSRIVVETPQNTAAPSESGAASSPPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPHV
QSDPEAIAMASGADDVLASAHVVPTLGEALSGVHWSIALTARTREYGPPRLAPRAAATQASAQVGTGDIALVFGNERTGL
ANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEQASEPSAQPQAEAGTLAQSDEIERMYVHLENALIALDF
LDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSGKPGGDA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=239, Percent_Identity=46.4435146443515, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1790865, Length=199, Percent_Identity=36.6834170854271, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30488; Mature: 30357

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPYGHSDEPSRIVVETPQNTAAPSESGAASSPPPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAIAMASGADDVLASAHVVPTLGEALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAATQASAQVGTGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEQASEPSAQPQAEAGTLAQSDEIERMYVHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCEEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSGKPGGDA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AIPYGHSDEPSRIVVETPQNTAAPSESGAASSPPPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAIAMASGADDVLASAHVVPTLGEALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAATQASAQVGTGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEQASEPSAQPQAEAGTLAQSDEIERMYVHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCEEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGIAKHILLKSGKPGGDA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA