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The map label for this gene is tilS [H]

Identifier: 116690096

GI number: 116690096

Start: 2304868

End: 2306289

Strand: Reverse

Name: tilS [H]

Synonym: Bcen2424_2075

Alternate gene names: 116690096

Gene position: 2306289-2304868 (Counterclockwise)

Preceding gene: 116690097

Following gene: 116690095

Centisome position: 66.2

GC content: 72.36

Gene sequence:

>1422_bases
GTGATCCCACCCACCGAATTCTCCGCCGACCGCGTCGTCCTCGACGCGGTCGGCGTTGCGCTTTCTGGTCTGCCGCCGGA
CGCGCGCATCGCGATCGCCTACAGCGGCGGCCTCGATTCGACCGTGCTGCTCGATGCGGCCGTGCGCGTCGCGGGCGCAT
CGCGCTGCGTCGCGCTGCACGTGCATCACGGCCTGAGCGCGAACGCCGATGCGTGGGTCGCGCATGCCGAAGGGCAAGCG
GCCCGGCTCGGTGTCGCATTCGAATCGATGCGCGTCGACGTGCCGCGCGACAGCGGGCTCGGCATCGAGGCGACCGCGCG
CGAGCGTCGCTACGCGGCGCTCGACGAGATGGGCGAACGCCATGGCGTGGCGGCGCTGTGGCTCGCGCAACATGCGGACG
ACCAGGCGGAGACGGTGCTGCTGCAACTGTTGCGCGGCGCGGGAATCGCGGGGCTCGCCGCGATGGCGCCGCGTTATCGA
CCCGACGGCGTGAGCATCGAGCGTGTACGTCCATTGCTGCGACTGTTGCGCGCACAACTCGAGCGCTATGCGGAGCAGCA
CGCGCTCGCGTGGATCGACGACGAATCGAACGGCGACACGCGCTATGCGCGCAATGCGCTGCGCGTCGACGTGCTGCCGG
CGCTGGCCGTGCATTTCCCGGGCTTTCGCGACGCGCTCGGCCGCGCGGCCCAGCATGCGGCGGCCGCTCAGCGGCTGCTC
GACGATCTGGCCGAGCTCGATTTCGCCGTCGCCGCGCGCGATGACGGTCACGCGCTGTCGCGCGACGCGCTGGCAGGATT
CGACGACACGCGCGGCGCGAACCTGCTGCGCTTCTGGATGCGCCGGCTCGGTTTGCCGGGCGCGTCGGCTGCCCGCCTGG
CCAACATGATGCGGCAACTGCGCGCGGCGCACGACGCGCATGCGCTGCGTGTCGATCACGCGGGGCAGTGTCTGCGGCTC
TATCGCGATGTCGTGTACTGGGAGGCGGGCGACAGCAGCGAGCCGGCCGACGACGGCACGGGTACGCCGCAGCCGGAGGC
GGCACTCGCGTGGGACGGACAGGAAGTGTGGCATCTGCCGGGGTGGCGCGGCACGTTCGTGTTCGCGCCCGCCGATGCGG
GGAGCGCCGACGCGGTGCCCGAGGCGCTGCTGCGCGGTACCCGGCTGGCGGCGCGTGCCCGTGCCGGCGGCGAACGGATG
CGCACGTCGCCGGGCGGGCCGGGCCGGACGCTGAAGAACCTGTTCCAGGAGCGCGGGGTGCCGGCCTGGCAACGCGACGT
GCCGCTGTTGTTCGCGGGTGACCGGCTGATCTACGTGCCGCGACTGGGCGTCAATCGCGACATCGGCGAGCCGGCCGACG
CGGCGCCCGGTGACGCGGCATGGCGCCGAATCGAATGGCGCCCCGATTTGCTGATCGCCTAG

Upstream 100 bases:

>100_bases
TTCGAGCGCCTGATGGCCTACGGCAAATTCAAGGAAACCACGCCCGGCGCATGACCGGCCGTGTATTCAAGGCGCGTGCC
GCTGCACGCGCGTCTCGCTC

Downstream 100 bases:

>100_bases
CAGCGGCAGGCGGTCGAACGCGCGATGGCGGGCCCATTTTCACCATTTGGTAAACAGCGCCGGAACGGCCGCCGACACGG
CGCGCGGCTTGTCAAGCGGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIPPTEFSADRVVLDAVGVALSGLPPDARIAIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEGQA
ARLGVAFESMRVDVPRDSGLGIEATARERRYAALDEMGERHGVAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSIERVRPLLRLLRAQLERYAEQHALAWIDDESNGDTRYARNALRVDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAVAARDDGHALSRDALAGFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRAAHDAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGTPQPEAALAWDGQEVWHLPGWRGTFVFAPADAGSADAVPEALLRGTRLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDIGEPADAAPGDAAWRRIEWRPDLLIA

Sequences:

>Translated_473_residues
MIPPTEFSADRVVLDAVGVALSGLPPDARIAIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEGQA
ARLGVAFESMRVDVPRDSGLGIEATARERRYAALDEMGERHGVAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSIERVRPLLRLLRAQLERYAEQHALAWIDDESNGDTRYARNALRVDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAVAARDDGHALSRDALAGFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRAAHDAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGTPQPEAALAWDGQEVWHLPGWRGTFVFAPADAGSADAVPEALLRGTRLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDIGEPADAAPGDAAWRRIEWRPDLLIA
>Mature_473_residues
MIPPTEFSADRVVLDAVGVALSGLPPDARIAIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEGQA
ARLGVAFESMRVDVPRDSGLGIEATARERRYAALDEMGERHGVAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSIERVRPLLRLLRAQLERYAEQHALAWIDDESNGDTRYARNALRVDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAVAARDDGHALSRDALAGFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRAAHDAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGTPQPEAALAWDGQEVWHLPGWRGTFVFAPADAGSADAVPEALLRGTRLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDIGEPADAAPGDAAWRRIEWRPDLLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=427, Percent_Identity=37.4707259953162, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51007; Mature: 51007

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPPTEFSADRVVLDAVGVALSGLPPDARIAIAYSGGLDSTVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
VHHGLSANADAWVAHAEGQAARLGVAFESMRVDVPRDSGLGIEATARERRYAALDEMGER
EECCCCCCCCCEEEECCCCCCEEEEEEEHEEEECCCCCCCCCEEHHHHHHHHHHHHHHHH
HGVAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGVSIERVRPLLRLLRAQL
CCCEEEEHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYAEQHALAWIDDESNGDTRYARNALRVDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAVAARDDGHALSRDALAGFDDTRGANLLRFWMRRLGLPGASAARLANMMRQL
HHHHHCCEEEEECCCCCHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAAHDAHALRVDHAGQCLRLYRDVVYWEAGDSSEPADDGTGTPQPEAALAWDGQEVWHLP
HHHHHHHEEEECCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEECC
GWRGTFVFAPADAGSADAVPEALLRGTRLAARARAGGERMRTSPGGPGRTLKNLFQERGV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCC
PAWQRDVPLLFAGDRLIYVPRLGVNRDIGEPADAAPGDAAWRRIEWRPDLLIA
CCHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC
>Mature Secondary Structure
MIPPTEFSADRVVLDAVGVALSGLPPDARIAIAYSGGLDSTVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
VHHGLSANADAWVAHAEGQAARLGVAFESMRVDVPRDSGLGIEATARERRYAALDEMGER
EECCCCCCCCCEEEECCCCCCEEEEEEEHEEEECCCCCCCCCEEHHHHHHHHHHHHHHHH
HGVAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGVSIERVRPLLRLLRAQL
CCCEEEEHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYAEQHALAWIDDESNGDTRYARNALRVDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAVAARDDGHALSRDALAGFDDTRGANLLRFWMRRLGLPGASAARLANMMRQL
HHHHHCCEEEEECCCCCHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAAHDAHALRVDHAGQCLRLYRDVVYWEAGDSSEPADDGTGTPQPEAALAWDGQEVWHLP
HHHHHHHEEEECCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEECC
GWRGTFVFAPADAGSADAVPEALLRGTRLAARARAGGERMRTSPGGPGRTLKNLFQERGV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCC
PAWQRDVPLLFAGDRLIYVPRLGVNRDIGEPADAAPGDAAWRRIEWRPDLLIA
CCHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA