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The map label for this gene is dmlR [H]

Identifier: 116690071

GI number: 116690071

Start: 2274717

End: 2275622

Strand: Reverse

Name: dmlR [H]

Synonym: Bcen2424_2050

Alternate gene names: 116690071

Gene position: 2275622-2274717 (Counterclockwise)

Preceding gene: 116690074

Following gene: 116690070

Centisome position: 65.32

GC content: 69.87

Gene sequence:

>906_bases
ATGGACCGGTTCAAACAGATCGAAACGTTCGCCGCGGTCGCGGCGAAAGGCAGCCTGTCGGCGGCTGCGCATGCGGAAGG
CGTTGCGCCGGCGATCATCGGCCGCCGCCTCGACGCGCTCGAGGAGCGGCTCGGCGTGAAGCTGCTGGTTCGCACGACGC
GCAAGCTCACGCTGACGTTCGAGGGTTCGGCGTTCCTCGAGGATTGCCAGCGCATCATTAACGACATGCAGAACGCGGAA
GCGAGCGTGTCCGCGGGCGGCGTCAAGGCGAGCGGGCACCTGCGGATTTCCGCGCCGGCCGGCTTCGGCCGGCGGCACGT
CGCGCCGCTCGTGCCCGAATTCAGCGGCGCGCATCCGGACGTGTCGGTCACGCTCGACCTGTCCGACCGGATGGTCGACC
TCGTCAACGAGGGGTTCGATTGCGCGGTGCGGCTCGGCGAGCTGCCCGACTCGTCGCTCGTGTCGCTGAAACTCGGCGAG
AACCGCCGCGTGTGCGTCGCGTCGCCCGCGTATCTCGCGCGGCGCGGCACGCCGGCCACGCTCGCGGAACTGGCGCGGCA
CAACTGCCTCGCGCTCGCGGCGAACGCAAACCAGCAGCGCGGCTGGTCGTTCCAGGAGGACGACAAGGTCGTGTCGATCC
GCGTGAACGGCACGATGGAATGCTCGGACGGCGCGGTGCTGCACGAGTGGTGCCTCGCAGGCCATGGCCTCGCGTGGCGC
TCGTGGTGGGAAGTGGGCGAGGACATCGCGGCCGGGCGGCTCGTCAGCGTGCTCGACGCGTTCGCCGCGCCGCCGATCGG
CATCCACGCGGTGTTTCCGCAGCGCCGGCATCTGCCGCTGCGGGTGCGCCTGTTTCTCGATTACCTGAAGCACACGTACG
AGCGGCCCGGCTATTGGGGCGAGTAG

Upstream 100 bases:

>100_bases
AAAAAGACGCTAAATATCACTTGATCTTTTACTTTCATATACCTAATCTGAGCGACTCACGTTATTTGAAAGGCGGCGAT
GCCGTCCTTTCGAGAGAGTC

Downstream 100 bases:

>100_bases
GCGACGCGCGCCCGCGTGTTTCCGATATGAGGGCGAACCCGCTGCCGATTCGGCCGGCGCGATTTCGCACACTACAATCG
TAAGAGGCATGCGCGCCGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWSFQEDDKVVSIRVNGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGEDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE

Sequences:

>Translated_301_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWSFQEDDKVVSIRVNGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGEDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE
>Mature_301_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWSFQEDDKVVSIRVNGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGEDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=35.5172413793103, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.1188811188811, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=32.5423728813559, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.3886925795053, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.616724738676, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=29.0969899665552, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=26.8817204301075, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=23.9057239057239, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=27.6, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.4550264550265, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=25.3424657534247, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32768; Mature: 32768

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00037 MYB_1 ; PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARRGTPAT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCHH
LAELARHNCLALAANANQQRGWSFQEDDKVVSIRVNGTMECSDGAVLHEWCLAGHGLAWR
HHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHCCCCHHH
SWWEVGEDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARRGTPAT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCHH
LAELARHNCLALAANANQQRGWSFQEDDKVVSIRVNGTMECSDGAVLHEWCLAGHGLAWR
HHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHCCCCHHH
SWWEVGEDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]