Click here to switch to the map view.

The map label for this gene is yjfH [C]

Identifier: 116690025

GI number: 116690025

Start: 2220724

End: 2221506

Strand: Reverse

Name: yjfH [C]

Synonym: Bcen2424_2004

Alternate gene names: 116690025

Gene position: 2221506-2220724 (Counterclockwise)

Preceding gene: 116690026

Following gene: 116690023

Centisome position: 63.76

GC content: 70.37

Gene sequence:

>783_bases
ATGAAAAGCATCACTTCCCGCGACAATCCGCTGTACAAGCGCCTGAAGGCGCTCGCGGGTTCGACGCCCCATCAGCGCCG
CAGCGGCCAGGCGCTGCTCGAAGGCTTCCATCTGGCAAGCGCGTACCTCGATACGGGCGCGACGCCCGAGCTGTGCGTCA
CGACCGAAGGCGCGCTCGGCCACGCCGAGGCGCAGGCGATCGTCGCACGCATCGACGCGCAGCGGATCGTCACGCTGCCG
GACGCGCTGTTCGGGCAGTTGTCGAACGTCGTCAACGGCGTCGGCTTCCTGCTGCTGGTCGACCGGCCGGCGCACCCGCT
GCCCGAACGCGTGACGCACACGTCGGTCGTGCTCGACGGCGTGCAGGATGCCGGCAACGTCGGCTCGATCCTGCGCAGCG
CGGCGGCGGCCGGCGTGCGTCACGTGTTCTGCGCGCCGGGGACGGCCTATGCGTGGTCGTCGAAGGTGCTGCGCTCGGGC
ATGGGCGCGCATTTCCTGCTGTCGATCCATGAGGACGTCGAAGCTGAGGCGCTGGCCGAACGCCTCGACGTCCCGGTCGC
GCTGACCGACTCGCACGGCGCGCAGGCGGTCTACGACTGCGACCTGTCGGGACCTGTCGCCTGGGTGTTCGGCAACGAAG
GTGCCGGCGTGTCGGCATTCTGGCGCGACGCGGCCACGCATCGCGTGACGATTCCGCAGCCGGGCGGGATGGAGTCGCTG
AACGTCGCGGCGGCGGCGGCAGTGTGCCTGTTCGAGCAGGTACGGCAGCAGCGGCCGGCGTGA

Upstream 100 bases:

>100_bases
ACGGCACCGCGAAACACCTTGCCGCACTTCGCGAGCACGGCCCGTGCGAAGCGCATCGGCGCTCGTTCGCGCCGGTTCGC
GCGGCGCTTGACCTGATTCG

Downstream 100 bases:

>100_bases
CGCCGCACGCGGTGCATGTGCCGTAAAACGAAACAAGGCCGCGTTTGACGCGGCCTTGTTGCTTTCGGCGACGGCCTGAC
GGCCGCCAGCGCTTCCGCGC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKSITSRDNPLYKRLKALAGSTPHQRRSGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARIDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPAHPLPERVTHTSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAEALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRPA

Sequences:

>Translated_260_residues
MKSITSRDNPLYKRLKALAGSTPHQRRSGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARIDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPAHPLPERVTHTSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAEALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRPA
>Mature_260_residues
MKSITSRDNPLYKRLKALAGSTPHQRRSGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARIDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPAHPLPERVTHTSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAEALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRPA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=301, Percent_Identity=29.2358803986711, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=30.3448275862069, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666840, Length=287, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27373; Mature: 27373

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSITSRDNPLYKRLKALAGSTPHQRRSGQALLEGFHLASAYLDTGATPELCVTTEGALG
CCCCCCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
HAEAQAIVARIDAQRIVTLPDALFGQLSNVVNGVGFLLLVDRPAHPLPERVTHTSVVLDG
HHHHHHHHHHHCHHHEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHC
VQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSGMGAHFLLSIHEDVEAEALAE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHCCCCCEEECCCCCCCCHH
NVAAAAAVCLFEQVRQQRPA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSITSRDNPLYKRLKALAGSTPHQRRSGQALLEGFHLASAYLDTGATPELCVTTEGALG
CCCCCCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
HAEAQAIVARIDAQRIVTLPDALFGQLSNVVNGVGFLLLVDRPAHPLPERVTHTSVVLDG
HHHHHHHHHHHCHHHEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHC
VQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSGMGAHFLLSIHEDVEAEALAE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHCCCCCEEECCCCCCCCHH
NVAAAAAVCLFEQVRQQRPA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]