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The map label for this gene is ppsA [H]

Identifier: 116690023

GI number: 116690023

Start: 2217029

End: 2219431

Strand: Reverse

Name: ppsA [H]

Synonym: Bcen2424_2002

Alternate gene names: 116690023

Gene position: 2219431-2217029 (Counterclockwise)

Preceding gene: 116690025

Following gene: 116690022

Centisome position: 63.71

GC content: 63.92

Gene sequence:

>2403_bases
ATGACTAACGCAGCAAACGTCGCAAAGGACCAGGCGTATGTAATTCCGTTCGAGCAGTTGCGGATGACCGATGTGGAAAT
CGTGGGCGGCAAGAATGCGTCGCTCGGCGAGATGATCAGCCAGCTTTCCGAAGCAGGCGTTCGCGTACCCACCGGTTTCG
CCACGACCGCGCTCGCATTCCGCGATTTCCTCAAGCACAACGACCTCACCGATCGCATCGCCAAGCGTCTCGAGTCGCTC
GACATCGACGACGTGAAGGCGCTCGCCGAAGCCGGTGCCGAAATCCGTAAATGGATCGTCGACGCACCGCTGCAGCCGCG
CCTCGAGCAGGAAATCCGCGCGCAGTTCGAAGTGCTGAAGAACGGCTCGCCGAGCGAGCTGTCGTTCGCGGTGCGCTCGT
CCGCGACCGCGGAAGATCTGCCCGACGCCTCGTTCGCCGGCCAGCAGGAGTCGTATCTGAACGTCGTCGGCATCGACGAC
GTGCTCGACCGCATGAAGCACGTGTTCGCGTCGCTGTACAACGACCGCGCGATCTCGTACCGCGTGCACAAGGGCTTCAC
GCACGCCGAAGTCGCGCTGTCGGCCGGTGTGCAGCGCATGGTCCGCTCGGACGTCGGCGCCGCCGGCGTGATGTTCACGA
TCGACACCGAATCGGGTTTCAAGGACGCCGTGTTCATCACGTCGAGCTACGGCCTGGGCGAAACCGTCGTGCAGGGCGCG
GTGAACCCGGACGAGTTCTACGTGTTCAAGACGACGCTCGCGCAGGACAAGTACCCGATCATCCGCCGCTCGATCGGCTC
GAAGCTGATCAAGATGGAATTCACGCAGCCGGGCGAGCCGGGCCGCGTGAAGACGGTCGACGTGCCGCACGAGCAGCGCA
ACCGCTACTCGATCACGGATGAGGACGTGATCGAGCTGGCGAAGTACGCGGTCATCATCGAGAAGCACTATCAGCGTCCG
ATGGACATCGAGTGGGGCAAGGACGGCCGCGACGGCAAGATCTTCATCCTGCAGGCACGTCCGGAAACGGTGAAGAGCCA
GGCATCCGGCAAGGCAGAGCAGCGCTTCAAGCTGAAGGGTCAGTCGCAGGTGCTCGCGACGGGTCGTGCGATCGGCCAGA
AGATCGGCGCGGGCCCCGTGCGCGTGATCCAGGATCCGTCGGAAATGGAACGCGTGCAGCCGGGCGACGTGCTGGTGGCC
GACATGACCGACCCGAACTGGGAGCCGGTGATGAAGCGCGCGGCGGCGATCGTCACGAACCGTGGCGGCCGTACCTGCCA
CGCGGCGATCATCGCGCGTGAACTCGGCGTGCCGGCCGTCGTCGGCTGCGGCGATGCGACCGACATCCTGAAGGACGGCG
CGCTCGTCACCGTGTCGTGCGCGGAAGGCGACGAAGGCAAGATCTACGACGGGCTGCTCGAGACGGAAGTCACGGAAGTG
CAGCGCGGCGAGCTGCCGGAAATCCCGGTCAAGATCATGATGAACGTCGGCAACCCGCAGCTCGCGTTCGACTTCTCGCA
ACTGCCGAACGGCGGCGTGGGTCTCGCGCGTCTCGAGTTCATCATCAACAACAACATCGGCGTTCACCCGAAGGCGATTC
TCGAGTACCCGAACATCGACCAGGACTTGAAGAAGGCGGTCGAGAGCGTGGCGCGCGGCCACGCGTCGCCGCGTCAGTTC
TACGTCGACAAGCTGACGGAAGGCGTCGCGACGATCGCGGCGGCGTTCTATCCGAAGCCGGTGATCGTGCGTCTGTCCGA
CTTCAAGTCGAACGAGTACAAGAAGCTGATCGGCGGTTCGCGTTACGAGCCGGACGAGGAAAACCCGATGCTGGGCTTCC
GCGGCGCGTCGCGCTACATCGCCGAGGACTTCGCGCAGGCGTTCGAGATGGAATGCCGTGCACTGAAGCGCGTGCGTGAC
GAGATGGGCCTGACCAACGTCGAGATCATGGTGCCGTTCGTGCGGACCGTGAAGCAGGCGGAGCGCGTGGTCGGCCTGCT
CGAGAAGTTCGGCCTGAAGCGTGGCGAAAACGGCCTGCGCCTTGTGATGATGTGCGAAGTCCCGACCAACGCGATCCTCG
CCGAAGAGTTCCTGGAACACTTCGACGGTTTCTCGATCGGCTCGAACGACCTCACGCAGCTCACGCTCGGCCTCGACCGC
GATTCGGGCATGGAACTGCTGGCAGTCGACTTCGACGAGCGCGACCCGGCCGTCAAGTTCCTGCTGAAGCGTGCGATCGA
TACGTGCCGCAAGATGGGCAAGTACGTCGGGATCTGCGGCCAGGGCCCGTCCGATCACCCGGACTTCGCGCAATGGCTGA
CGGACGAAGGCATCGTGTCGATCTCGCTGAACCCCGACACGATCATCGATACGTGGCAGGCGCTCGCCGCCAACCGCAAG
TAA

Upstream 100 bases:

>100_bases
TATCTAACAGGTTGCGCAAGACGCGCCGCGCCTTTTTGCAAGCGCCCGTGTTCGAGCCCACTTGCGCGATCGAGCATTTT
TTTCACACTTAGGGGCTTGT

Downstream 100 bases:

>100_bases
CGAGTCGTGATGCAACTTCGACGAAAGGGCGGTCCGCCGCACTTTCGTTGAAGGCAAAATGCAAGGTTCGTGCTATAAAC
ACCCCGGTAAGCGCCGGGGT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLSEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESL
DIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLKNGSPSELSFAVRSSATAEDLPDASFAGQQESYLNVVGIDD
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDEDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVA
DMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGALVTVSCAEGDEGKIYDGLLETEVTEV
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNGGVGLARLEFIINNNIGVHPKAILEYPNIDQDLKKAVESVARGHASPRQF
YVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECRALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLEHFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALAANRK

Sequences:

>Translated_800_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLSEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESL
DIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLKNGSPSELSFAVRSSATAEDLPDASFAGQQESYLNVVGIDD
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDEDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVA
DMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGALVTVSCAEGDEGKIYDGLLETEVTEV
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNGGVGLARLEFIINNNIGVHPKAILEYPNIDQDLKKAVESVARGHASPRQF
YVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECRALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLEHFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALAANRK
>Mature_799_residues
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLSEAGVRVPTGFATTALAFRDFLKHNDLTDRIAKRLESLD
IDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLKNGSPSELSFAVRSSATAEDLPDASFAGQQESYLNVVGIDDV
LDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGAV
NPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDEDVIELAKYAVIIEKHYQRPM
DIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVAD
MTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGALVTVSCAEGDEGKIYDGLLETEVTEVQ
RGELPEIPVKIMMNVGNPQLAFDFSQLPNGGVGLARLEFIINNNIGVHPKAILEYPNIDQDLKKAVESVARGHASPRQFY
VDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECRALKRVRDE
MGLTNVEIMVPFVRTVKQAERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLEHFDGFSIGSNDLTQLTLGLDRD
SGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVSISLNPDTIIDTWQALAANRK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=788, Percent_Identity=64.9746192893401, Blast_Score=1053, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=399, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=32.2916666666667, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI48994992, Length=411, Percent_Identity=25.5474452554745, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=23.9024390243902, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=31.6455696202532, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 88141; Mature: 88009

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLSEAGVRVPTGFATTALAF
CCCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLKHNDLTDRIAKRLESLDIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLK
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGSPSELSFAVRSSATAEDLPDASFAGQQESYLNVVGIDDVLDRMKHVFASLYNDRAISY
CCCCCCEEEHHHCCCCHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHCC
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCH
EDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHEEECC
QSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVADMTDPNWEPVMKRAAAIVTN
CCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDILKDGALVTVSCAEGDEGKIYDGLLETEVTEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNGGVGLARLEFIINNNIGVHPKAILEYPNID
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCC
QDLKKAVESVARGHASPRQFYVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIAEDFAQAFEMECRALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLEHFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCEEE
ISLNPDTIIDTWQALAANRK
EECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLSEAGVRVPTGFATTALAF
CCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLKHNDLTDRIAKRLESLDIDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRAQFEVLK
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGSPSELSFAVRSSATAEDLPDASFAGQQESYLNVVGIDDVLDRMKHVFASLYNDRAISY
CCCCCCEEEHHHCCCCHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITSSYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHCC
VNPDEFYVFKTTLAQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCH
EDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQASGKAEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHEEECC
QSQVLATGRAIGQKIGAGPVRVIQDPSEMERVQPGDVLVADMTDPNWEPVMKRAAAIVTN
CCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDILKDGALVTVSCAEGDEGKIYDGLLETEVTEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPEIPVKIMMNVGNPQLAFDFSQLPNGGVGLARLEFIINNNIGVHPKAILEYPNID
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCC
QDLKKAVESVARGHASPRQFYVDKLTEGVATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIAEDFAQAFEMECRALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLEKFGLKRGENGLRLVMMCEVPTNAILAEEFLEHFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFLLKRAIDTCRKMGKYVGICGQGPSDHPDFAQWLTDEGIVS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCEEE
ISLNPDTIIDTWQALAANRK
EECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]