| Definition | Burkholderia cenocepacia HI2424 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008542 |
| Length | 3,483,902 |
Click here to switch to the map view.
The map label for this gene is mrcA [H]
Identifier: 116689969
GI number: 116689969
Start: 2165761
End: 2168280
Strand: Reverse
Name: mrcA [H]
Synonym: Bcen2424_1948
Alternate gene names: 116689969
Gene position: 2168280-2165761 (Counterclockwise)
Preceding gene: 116689974
Following gene: 116689964
Centisome position: 62.24
GC content: 67.62
Gene sequence:
>2520_bases ATGCCAATTATCAAACGACCGCCTTCTTCTTCCGACAGGAACGAGCCCCACTACACGCTGCGCCGTTCGCCGTCCGGAGC CTATTACCCCGATGACGACGATCGCGACGACGGCCGCCGCGCGCAGCAGCGCAGTGGCGGTGGAGGGCGCCGGCGCACGT TCGGTTCGCGCGTCGCGCTGTGGTTCGTCGGCCTGTTCGCGACGCTCGCGGTCGTCGGCGCGCTGATCGTCGGCTACGCG CTGGTCGTGATGGCGCCGCAACTGCCGTCGCTCGATGCGCTGACCAATTACCAGCCGAAGGTGCCGCTGCGCGTGTTTAC CGCCGATCACGTGCTGATCGGCGAGTTCGGCGAGGAGCGCCGCAGCCTCGTGCGCTTCCAGGACATTCCCGACGTGATGA AGAAGGCCGTGCTCGCCATCGAGGACTACCGCTTCTACGAGCACGGCGGCGTCGATTTCCTCGGCATCCTGCGCGCGGGC GTGGCCGACCTGATGCACGGCGGCGCGCGCCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTC GAGCGAGAAGACCTACACGCGCAAGATCTACGAAATGCTGCTCGCGTACAAGATCGAGAAGGCCCTGACGAAGGACCAGA TCCTCGAGCTGTACATGAACCAGATCTATCTCGGCCAGCGTTCGTACGGCTTCGCGGCCGCGGCGCGCGTGTATTTCGGC AAGGACCTGAAGGACATCACGCTCGCGGAAGCGGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCGTACAACCCGGT CGTCAATCCGAAGCGCGCGAAGGTGCGTCAGGAATACATCCTGAAGCGCATGCTCGACATCGGCTACATCACGCAGCCGC AGTACGACGAGGCGATCAAGGAAGAGATCCACGTGCGCACGCCGGGCAACCAGTACGCGGTGCACGGCGAGTACGTCGCC GAGATGGTGCGGCAGATGATGTACCAGCAGTACAAGGACGAAACCTACACGCGCGGGCTGACCGTCACGACGACGATCAA CTCGGCCGACCAGGAAGCCGCGTATCAGGCCGTGCGTCGCGGCATTCTCGACTACGAGCGCCGTCACGGCTATCGCGGCC CCGAAGCGTCGATCAACCTGCCTGCGGCCGGCGACGAACGCGACGAGGCGATCGACGATGCGCTCGCCGATCACCCGGAC AACGGCGACCTGCAATCGGCGGTCGTGCTGTCGGCATCGCCGAACGCGGTCGAGGTGCAGTTCGTCGGCGGCGCGACCAC GACGATCGGCCCGGCCGGGCTGCGCTTCGTGTCGGCCGCGCTGGGCGCCCGTGCGAACAACGCGCTGCGTATCAAGCCGG GCTCGGTCGTGCGCGTGCTGAAGGACGGCAAGACGGGCTGGCAGGTGGTGCAGCTGCCGCAGGTCGAAGGCGCGCTCGTC GCGATCGCGCCGCAGGACGGTGCGATCCGCTCGCTCGTCGGCGGCTTCGACTTCAACAAGAGCAAGTTCAACCACGTGAC GCAGGCGTGGCGGCAGCCGGGCTCATCGTTCAAGCCGTTCATCTACTCGGCGTCGCTCGACAAGGGGCTCGGGCCGGCGA CGATGATCAACGACGCGCCGCTGTACTTCCCGCCGAGCGTGCCGGGCGGCACCGCGTGGGAGCCGAAGGACGACGATCAG CCGGACGGCCCGATGACGATGCGCACCGGCCTGCAGCGTTCGAAGAACCTGGTGTCGATCCGGATCCTCGCGTCGATCGG CACGCAGTACGCGCAGCAGTACGTGACGCAGCGCTTCGGTTTCGATCCGGCGAAGACGCCGCCTTACCTGCCGATGGCGC TCGGCGCGGGCCTCGTGACGCCGTTGCAGCTCGCGACCGGCTACGCGGTGTTCGCGAACGGCGGCTACAAGGTCGATCCG TACCTGATCGCCGAAGTCGACGACGCGCGCGGCCAGCCGCTGCAGAAGTCGCAGCCGGTGGTCGCGGGCGGCACGGCGCC GCGCACGATCGAAGGCCGCAACGCGTACGTGATGAACAGCCTGCTGCATTCGGTCGCGACCGCCGGCACGGGCGCGGGCA CCAACGCGCTGGGCCGCAACGACCTGCAGGGCAAGACGGGTACGACGAACGATGCGAAGGACGGCTGGTTCGCCGGCTAC CAGCAGTCGCTCGTCGCGGTCGCGTGGATGGGCTTCGACCAGCCGAAGAGCCTGGGCAGCCGCGAATTCGGCGCGCAGCT CGCGCTGCCGATCTGGGTGAACTACATGCGCACCGCGCTGAACGGCGTGCCCGAGCAGCAGATGCCGATGCCGGACGGCC TGACGACGATCGACGGCGAGCTGTACTACGCCGATCGCACGCCGGGCAACGGCTTCGTCGCGAACGTCGACATCAACCCG GCGGAGAACGCGATCAGCGCGAACGACGCCCTCGGTTCGGCCGGCGCGGCCGGGCTGGCGCCGCCGCCCGTCACGCCGCA GGAGAAGCAGCAGATCATGGACATGTTCGAGAGCAAGTAG
Upstream 100 bases:
>100_bases TCTGTCGACGTGACAGCCGCATCTCGATGCACCCACTCTGATTCATCCGCTTGCATTTCCGCATGCGTCCGAGGGCGCCA CGTACGCCCGCCATTCCGAC
Downstream 100 bases:
>100_bases GCGGTGCGCTGTTCTTCAGCCATGCGACGGGCGCCTTCGGGCGCCCGTTTTTTATCCGCGGCCGGCAGCGGAAGACGTCG AATCAGAACGACGCGCAGGC
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 839; Mature: 838
Protein sequence:
>839_residues MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDGRRAQQRSGGGGRRRTFGSRVALWFVGLFATLAVVGALIVGYA LVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQDIPDVMKKAVLAIEDYRFYEHGGVDFLGILRAG VADLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIEKALTKDQILELYMNQIYLGQRSYGFAAAARVYFG KDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLDIGYITQPQYDEAIKEEIHVRTPGNQYAVHGEYVA EMVRQMMYQQYKDETYTRGLTVTTTINSADQEAAYQAVRRGILDYERRHGYRGPEASINLPAAGDERDEAIDDALADHPD NGDLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVSAALGARANNALRIKPGSVVRVLKDGKTGWQVVQLPQVEGALV AIAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATMINDAPLYFPPSVPGGTAWEPKDDDQ PDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDP YLIAEVDDARGQPLQKSQPVVAGGTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRNDLQGKTGTTNDAKDGWFAGY QQSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTTIDGELYYADRTPGNGFVANVDINP AENAISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK
Sequences:
>Translated_839_residues MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDGRRAQQRSGGGGRRRTFGSRVALWFVGLFATLAVVGALIVGYA LVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQDIPDVMKKAVLAIEDYRFYEHGGVDFLGILRAG VADLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIEKALTKDQILELYMNQIYLGQRSYGFAAAARVYFG KDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLDIGYITQPQYDEAIKEEIHVRTPGNQYAVHGEYVA EMVRQMMYQQYKDETYTRGLTVTTTINSADQEAAYQAVRRGILDYERRHGYRGPEASINLPAAGDERDEAIDDALADHPD NGDLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVSAALGARANNALRIKPGSVVRVLKDGKTGWQVVQLPQVEGALV AIAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATMINDAPLYFPPSVPGGTAWEPKDDDQ PDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDP YLIAEVDDARGQPLQKSQPVVAGGTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRNDLQGKTGTTNDAKDGWFAGY QQSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTTIDGELYYADRTPGNGFVANVDINP AENAISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK >Mature_838_residues PIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDGRRAQQRSGGGGRRRTFGSRVALWFVGLFATLAVVGALIVGYAL VVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQDIPDVMKKAVLAIEDYRFYEHGGVDFLGILRAGV ADLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIEKALTKDQILELYMNQIYLGQRSYGFAAAARVYFGK DLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLDIGYITQPQYDEAIKEEIHVRTPGNQYAVHGEYVAE MVRQMMYQQYKDETYTRGLTVTTTINSADQEAAYQAVRRGILDYERRHGYRGPEASINLPAAGDERDEAIDDALADHPDN GDLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVSAALGARANNALRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVA IAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATMINDAPLYFPPSVPGGTAWEPKDDDQP DGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPY LIAEVDDARGQPLQKSQPVVAGGTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRNDLQGKTGTTNDAKDGWFAGYQ QSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTTIDGELYYADRTPGNGFVANVDINPA ENAISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=817, Percent_Identity=40.1468788249694, Blast_Score=531, Evalue=1e-152, Organism=Escherichia coli, GI1786343, Length=265, Percent_Identity=35.4716981132075, Blast_Score=144, Evalue=2e-35, Organism=Escherichia coli, GI1788867, Length=229, Percent_Identity=32.3144104803493, Blast_Score=112, Evalue=1e-25, Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=3e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 91260; Mature: 91128
Theoretical pI: Translated: 7.86; Mature: 7.86
Prosite motif: PS00639 THIOL_PROTEASE_HIS
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDGRRAQQRSGGGGRRRTFGSRVAL CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH WFVGLFATLAVVGALIVGYALVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEER HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCEEEECCCCHHH RSLVRFQDIPDVMKKAVLAIEDYRFYEHGGVDFLGILRAGVADLMHGGARQGASTITMQV HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH ARNFFLSSEKTYTRKIYEMLLAYKIEKALTKDQILELYMNQIYLGQRSYGFAAAARVYFG HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC KDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLDIGYITQPQYDEAIK CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH EEIHVRTPGNQYAVHGEYVAEMVRQMMYQQYKDETYTRGLTVTTTINSADQEAAYQAVRR HHEEEECCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCHHHHHHHHHHH GILDYERRHGYRGPEASINLPAAGDERDEAIDDALADHPDNGDLQSAVVLSASPNAVEVQ HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEE FVGGATTTIGPAGLRFVSAALGARANNALRIKPGSVVRVLKDGKTGWQVVQLPQVEGALV EECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCEEEEECCCCCEEE AIAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATMINDAP EEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCC LYFPPSVPGGTAWEPKDDDQPDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFG CEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHC FDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPYLIAEVDDARGQPLQKSQPV CCCCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEEECCCCCCCCCCCCCE VAGGTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRNDLQGKTGTTNDAKDGWFAGY EECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH QQSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTTIDGE HHHHHHHHHCCCCCCHHCCCHHHCCEEEHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCE LYYADRTPGNGFVANVDINPAENAISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK EEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC >Mature Secondary Structure PIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDGRRAQQRSGGGGRRRTFGSRVAL CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH WFVGLFATLAVVGALIVGYALVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEER HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCEEEECCCCHHH RSLVRFQDIPDVMKKAVLAIEDYRFYEHGGVDFLGILRAGVADLMHGGARQGASTITMQV HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH ARNFFLSSEKTYTRKIYEMLLAYKIEKALTKDQILELYMNQIYLGQRSYGFAAAARVYFG HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC KDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLDIGYITQPQYDEAIK CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH EEIHVRTPGNQYAVHGEYVAEMVRQMMYQQYKDETYTRGLTVTTTINSADQEAAYQAVRR HHEEEECCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCHHHHHHHHHHH GILDYERRHGYRGPEASINLPAAGDERDEAIDDALADHPDNGDLQSAVVLSASPNAVEVQ HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEE FVGGATTTIGPAGLRFVSAALGARANNALRIKPGSVVRVLKDGKTGWQVVQLPQVEGALV EECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCEEEEECCCCCEEE AIAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATMINDAP EEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCC LYFPPSVPGGTAWEPKDDDQPDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFG CEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHC FDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPYLIAEVDDARGQPLQKSQPV CCCCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEEECCCCCCCCCCCCCE VAGGTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRNDLQGKTGTTNDAKDGWFAGY EECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH QQSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTTIDGE HHHHHHHHHCCCCCCHHCCCHHHCCEEEHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCE LYYADRTPGNGFVANVDINPAENAISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK EEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA