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The map label for this gene is ispF

Identifier: 116689963

GI number: 116689963

Start: 2157890

End: 2158375

Strand: Reverse

Name: ispF

Synonym: Bcen2424_1942

Alternate gene names: 116689963

Gene position: 2158375-2157890 (Counterclockwise)

Preceding gene: 116689964

Following gene: 116689958

Centisome position: 61.95

GC content: 68.93

Gene sequence:

>486_bases
ATGGATTTCAGAATCGGACAAGGCTATGACGTGCACCAGCTCGTCGAAGGGCGCCCCCTCATCATCGGCGGCGTGACGAT
TCCGTACGAGCGCGGCCTGCTCGGCCACTCGGACGCGGACGTGCTGCTGCATGCGATCACCGACGCGCTGTTCGGCGCGG
CGGCGCTCGGCGACATCGGCCGCCATTTCTCCGACACGGACGCGGCGTTCAAGGGCGCGGACAGCCGCGTGCTGCTGCGC
GCGTGCGCCGAGCGCGTGAAGGCGGCCGGCTTCACGATCCAGAACGTCGACAGCACCGTGATCGCGCAGGCGCCGAAGCT
CGCGCCGCATATCGACGGGATGCGTGCGAACATCGCGGCGGATCTCGGGCTGCCGCTCGAGCGCGTGAACGTGAAGGCGA
AGACCAACGAGAAGCTCGGCTATCTCGGTCGCGGCGAGGGGATCGAGGCGCAGGCCGCCGCGCTGCTGGTCAAGCAGGGC
GGCTGA

Upstream 100 bases:

>100_bases
TCAAGGTCACGTACCCGGAAGATTTCGCGCTCGCGGAGGCGATCCTCGCGCGCCCCGCGAACGCTTCCTGACTTTCCCCC
CATCTCAAACCGGATTACGC

Downstream 100 bases:

>100_bases
CGGGCCGTCGCGGTGTGCGGTTCGCGCCGCGCGCCGGGCGCCGTGCCGCCGAAATGAAAAATGCCACGCGAATGCGTGGC
ATTTTCGTTTGCAGCAGGCG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ACAERVKAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G

Sequences:

>Translated_161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ACAERVKAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G
>Mature_161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ACAERVKAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=64.7435897435898, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BURCA (Q1BHA4)

Other databases:

- EMBL:   CP000380
- RefSeq:   YP_625974.1
- ProteinModelPortal:   Q1BHA4
- SMR:   Q1BHA4
- STRING:   Q1BHA4
- GeneID:   4096871
- GenomeReviews:   CP000380_GR
- KEGG:   bcn:Bcen_6137
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   BCEN331271:BCEN_6137-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16924; Mature: 16924

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDAAFKGADSRVLLRACAERVKAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAA
CCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCHHCEEC
DLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQGG
CCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHHEEEEECCC
>Mature Secondary Structure
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDAAFKGADSRVLLRACAERVKAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAA
CCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCHHCEEC
DLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQGG
CCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA