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The map label for this gene is norM [H]

Identifier: 116689114

GI number: 116689114

Start: 1204123

End: 1205511

Strand: Reverse

Name: norM [H]

Synonym: Bcen2424_1091

Alternate gene names: 116689114

Gene position: 1205511-1204123 (Counterclockwise)

Preceding gene: 116689115

Following gene: 116689113

Centisome position: 34.6

GC content: 70.55

Gene sequence:

>1389_bases
ATGTCGCATTCCGGTCTTACGCGGACGGCCGCCGCGCCGCCGTCCCTGTCCAGTCATGCCGCCGATACCGCGCGCCTCGC
CGCACCGCTCGCGATCGCGCAACTCTCCCAGATGGCGATGAGCGTCACCGACACGGTGCTGCTCGGTTCGCTCGGCCCCG
ATTCGCTCGCGGCAGGCGGGCTCGGCGCGAACTTCTTCTTCGTGATCGTGACGATCCTGCAAGGCGTGCTGTCGTCGGTC
AGCGTGAGCGTCGCGCACGCGCGCGGCGCGCAGGCCGAGGACCGCGTGCCGCACATCTACTGGACGGGCTTCGCGCTGTC
CGTGCTGCTCGCGATTCCGGCAATCGTCGCGCTGTCGCTGGCCGAACCGATCTTGCTGATGTTCCACGAGCCGCCGCTGC
TCGCGCATCACGTCGGCGAATACACGGGCATCCTGCGCTACGCGGCGCTCGGCAGCCTGATCGGCGTCGGGCTGATGCGC
GCGTTCCTGCCCGCGATCGGCGCCGCGCGTCGGCTCCTGTGGGTGTCGATCGGCGGCGTCGGCGTGAACGGCGTGCTGAA
CTACGGGCTGATCCACGGCGCATTCGGGCTGCCGCGCTTGGGCTTTCTCGGCTCGGCCGTCGCGACGACGATCACGATCT
GGCTCACCGCGCTCGCCCTCATCTGGCTGCTGCACGGCCGGCAGCGCTTCCGCCATTTCGTGACCGCCGCGCGCCCGAAG
CTGCCCGTGATGGGCGAGCTGATCGGCATAGGCTGGCCGGTGGCGATCACGTACGGGGTCGAATCGACGCTGTTCCTCGC
CACCGGTCTCACGGTCGGCGTGCTGGGCGCGACGTCGCTCGCCGCGCACCAGATCGCGCTGAACGTCGCGTCGGTATCGT
TCATGGTGCCGCTTGCGATCGGCCAGGCAGCCAACGTGCGCGTCGGCTACTGGATCGGCGCGGGCTCGCCCGTCGCCGCG
CGGCACGCGGGCTTCGTCGCGCTGGCGCTCGGCGTCGGCTTCATGTCGCTGTCGGGCCTCGTGATGATCGTCGCGCCGCA
CACGATCGTCGGCCTGTACCTGCATCTCGACGATCCGGCCAACGCGGCCACGGTGTCACTCGCCGCGTCGCTGCTCGGCA
TCGCCGCGGTGTTCCAGATCGTCGACGGCATGCAGACCGTCGCGTCGGGTGCGCTGCGCGGCCTGAAGGACACGCGCATC
CCGATGCTCGCCGCGACCTTTGGCTACTGGGGCATCGGCTTCCCGACCGGCTACTGGTTCGCGTTCCATGCGGGCCTGGG
CGCGCGCGGCCTGTGGTGGGGACTCGCGGCCGGGCTCGCGAGCGTCGCCGCGCTGATGGCCTGGCGCTTTCACCGCAAGA
CCACTTCGCTGATCGCGACGGCCCGCTAG

Upstream 100 bases:

>100_bases
GGCGCACGCGTTCGACACGGTACGAATAAAGCCGTAAGCTAGCCGTCTCTCAAAATAAATACATATAGCGCGGGCCCGCA
CCCGCGCGAGCGCCCATTCC

Downstream 100 bases:

>100_bases
GGGCTGGTCGCGCTCGTCACGGGCTTGCGCCGTCCCCAGGCGCGACACGACGCGAAATCGCGACCGGCGCTATGCTTGTC
GGTTGAACCCCTCGAGACGC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTILQGVLSSV
SVSVAHARGAQAEDRVPHIYWTGFALSVLLAIPAIVALSLAEPILLMFHEPPLLAHHVGEYTGILRYAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPVMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWIGAGSPVAA
RHAGFVALALGVGFMSLSGLVMIVAPHTIVGLYLHLDDPANAATVSLAASLLGIAAVFQIVDGMQTVASGALRGLKDTRI
PMLAATFGYWGIGFPTGYWFAFHAGLGARGLWWGLAAGLASVAALMAWRFHRKTTSLIATAR

Sequences:

>Translated_462_residues
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTILQGVLSSV
SVSVAHARGAQAEDRVPHIYWTGFALSVLLAIPAIVALSLAEPILLMFHEPPLLAHHVGEYTGILRYAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPVMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWIGAGSPVAA
RHAGFVALALGVGFMSLSGLVMIVAPHTIVGLYLHLDDPANAATVSLAASLLGIAAVFQIVDGMQTVASGALRGLKDTRI
PMLAATFGYWGIGFPTGYWFAFHAGLGARGLWWGLAAGLASVAALMAWRFHRKTTSLIATAR
>Mature_461_residues
SHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTILQGVLSSVS
VSVAHARGAQAEDRVPHIYWTGFALSVLLAIPAIVALSLAEPILLMFHEPPLLAHHVGEYTGILRYAALGSLIGVGLMRA
FLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPKL
PVMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWIGAGSPVAAR
HAGFVALALGVGFMSLSGLVMIVAPHTIVGLYLHLDDPANAATVSLAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIP
MLAATFGYWGIGFPTGYWFAFHAGLGARGLWWGLAAGLASVAALMAWRFHRKTTSLIATAR

Specific function: Multidrug efflux pump. Confers probably resistance to the cationic peptide polymyxin B (PMB) [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=427, Percent_Identity=26.463700234192, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI153792564, Length=430, Percent_Identity=24.1860465116279, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI38261960, Length=462, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI48994890, Length=447, Percent_Identity=32.662192393736, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI226510954, Length=451, Percent_Identity=25.7206208425721, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47815; Mature: 47684

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTILQGVLSSVSVSVAHARGAQAEDRVPHIYWTGFALSVLLAIPAIVALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
AEPILLMFHEPPLLAHHVGEYTGILRYAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
HCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPVMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAI
CCHHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWIGAGSPVAARHAGFVALALGVGFMSLSGLVMIVAPHTIVGLYLHLDDPA
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEECCCC
NAATVSLAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIPMLAATFGYWGIGFPTGYWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHH
AFHAGLGARGLWWGLAAGLASVAALMAWRFHRKTTSLIATAR
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTILQGVLSSVSVSVAHARGAQAEDRVPHIYWTGFALSVLLAIPAIVALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
AEPILLMFHEPPLLAHHVGEYTGILRYAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
HCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPVMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAI
CCHHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWIGAGSPVAARHAGFVALALGVGFMSLSGLVMIVAPHTIVGLYLHLDDPA
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEECCCC
NAATVSLAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIPMLAATFGYWGIGFPTGYWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHH
AFHAGLGARGLWWGLAAGLASVAALMAWRFHRKTTSLIATAR
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12399047 [H]