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The map label for this gene is nuoF2 [H]

Identifier: 116689055

GI number: 116689055

Start: 1144613

End: 1146190

Strand: Reverse

Name: nuoF2 [H]

Synonym: Bcen2424_1032

Alternate gene names: 116689055

Gene position: 1146190-1144613 (Counterclockwise)

Preceding gene: 116689056

Following gene: 116689054

Centisome position: 32.9

GC content: 70.72

Gene sequence:

>1578_bases
ATGACGACCCGCATCTACGTTCCGCGCGATTCGTCCGCGCTGGCGCTCGGCGCCGACGCGCTCGCCGCCGCGATCGTGGC
CGAAGCCGAACGGCGCGGCGTCACGGTCGAACTGATCCGCAACGGTTCGCGCGGGCTGCTGTGGCTCGAGCCGCTCGTCG
AGGTCGGGACGGCCGCGGGCCGCGTCGGCTATGCGAACCTGTCGGCCGCCGACGTGCCGGCCCTGTTCGACGCCGGCTGG
CTCGAAGGCGGCACGCATCCGAGCGGCGTCGGCCTCGTCGACGCGCTGCCCTACCTCGCCCGTCAGCAGCGGCTCACGTT
CGCGCGGATCGGCCTGACCGATCCGCTGTCGATCGACGACTATCTGCAACACGGCGGCCTCGCCGGCCTGAAGAACGCAC
TGGCGCTCGATGGCGACGCCGCCTGCGAAACGCTGATCGAATCGGGGCTGCGCGGCCGCGGCGGCGCGGCGTTCCCGGCC
GGCATCAAGTGGCGTACCGTGCGGCAAGCACGCGCCGCGCAGAAATACATCGTCTGCAACGCGGACGAAGGCGATTCGGG
CACGTTCTCCGATCGCCTGCTGATGGAATGCGATCCGTACTGCCTGATCGAAGGGATGATCATCGCGGGCGTCGCCACCG
GCGCGACGGTCGGCTACATCTATGTGCGCAGCGAATACCCGCACGCGATCGCGACGCTCGAAGCCGCGATCGTCCGCGCG
CGTGAAGCCGGCTGGCTCGGCGAGCACGTGCTCGGCTCCGCGCATGCGTTCGAGCTGCACGTCGCGAAAGGCGCGGGCTC
GTACGTATGCGGCGAGGAAACGGCGCTGCTCGAATCGCTCGAGGGCAAGCGCGGCGTCGTGCGCGCGAAGCCGCCGCTGC
CCGCGCTCGCGGGCCTGTTCGGCCAGCCGACCGTGATCAACAACGTGATCACGCTCGCGACGGCGCCCGTGATCTTCGCG
CGCGGCGCCGCGTTCTATCGCGACTACGGGATGGGCCGCTCGCGCGGCACGCTGCCGTTCCAGCTCGCGGGCAACATCCG
CTACGGCGGCCTCGTCGAGCTCGCGTTCGGCGTCACGCTGCGCGAACTGCTGTTCGACTTCGGCGGCGGCACGGCCAGCG
GCCGGCCGGCACGCGCCGCGCAGGTCGGCGGCCCGCTCGGCACCTACCTGCCCGACCACCAGTGGGACGTGCCGCTCGAC
TACGAGGCGTACACGGCGATCGGCGCGGTCGTCGGCCACGGCGGCATCGTGCTGCACGACGACACGTCGAATCTCGCCGA
GCTGGCCGAATACGCGATGAAGTTCTGCGCGATCGAATCGTGCGGCAAGTGCACGCCGTGCCGGATCGGTTCGACGCGCG
GCGTCGAGACGATCGCGCGGATTCGCCACGGTGATACGTCGGAGCGGCAGGTCACGCTGTTGCGCGACCTGTGCGACACC
ATGCTCGCCGGCTCGCTGTGCGCGATGGGCGGCATGACGCCGTACCCGGTGCTGTCCGCACTCGACCATTTCCCCGAAGA
TTTCGGGCTCGCCGCCGGCAAGGATGCCGCGTCGGACCCGGTCAAGGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGTCGATGACGGTCAACGGCGTGCTCCACGCCAAGGTCACGCCGGAGAAGTTCGACGCGCTGCTGGCCGACGCGGTC
GCCCACACGCCGGAGGCCGC

Downstream 100 bases:

>100_bases
CCCAGAAGGAGCCCTGCATGTCCCTCGACACGAACAACGTCCGCCAAGGCGGCTGCGGCTCGGGCCAATGCGCGTGCAAG
AGCGCCGCACAGGCGCGCGC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTTRIYVPRDSSALALGADALAAAIVAEAERRGVTVELIRNGSRGLLWLEPLVEVGTAAGRVGYANLSAADVPALFDAGW
LEGGTHPSGVGLVDALPYLARQQRLTFARIGLTDPLSIDDYLQHGGLAGLKNALALDGDAACETLIESGLRGRGGAAFPA
GIKWRTVRQARAAQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIVRA
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFA
RGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDHQWDVPLD
YEAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRHGDTSERQVTLLRDLCDT
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGKDAASDPVKAAA

Sequences:

>Translated_525_residues
MTTRIYVPRDSSALALGADALAAAIVAEAERRGVTVELIRNGSRGLLWLEPLVEVGTAAGRVGYANLSAADVPALFDAGW
LEGGTHPSGVGLVDALPYLARQQRLTFARIGLTDPLSIDDYLQHGGLAGLKNALALDGDAACETLIESGLRGRGGAAFPA
GIKWRTVRQARAAQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIVRA
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFA
RGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDHQWDVPLD
YEAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRHGDTSERQVTLLRDLCDT
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGKDAASDPVKAAA
>Mature_524_residues
TTRIYVPRDSSALALGADALAAAIVAEAERRGVTVELIRNGSRGLLWLEPLVEVGTAAGRVGYANLSAADVPALFDAGWL
EGGTHPSGVGLVDALPYLARQQRLTFARIGLTDPLSIDDYLQHGGLAGLKNALALDGDAACETLIESGLRGRGGAAFPAG
IKWRTVRQARAAQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIVRAR
EAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFAR
GAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDHQWDVPLDY
EAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRHGDTSERQVTLLRDLCDTM
LAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGKDAASDPVKAAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=426, Percent_Identity=34.5070422535211, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI20149568, Length=389, Percent_Identity=36.5038560411311, Blast_Score=252, Evalue=5e-67,
Organism=Escherichia coli, GI1788620, Length=398, Percent_Identity=38.9447236180905, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17531727, Length=420, Percent_Identity=36.1904761904762, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19920794, Length=389, Percent_Identity=36.2467866323907, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI45550452, Length=410, Percent_Identity=36.0975609756098, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24653819, Length=348, Percent_Identity=31.3218390804598, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=28.9156626506024, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55076; Mature: 54945

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRIYVPRDSSALALGADALAAAIVAEAERRGVTVELIRNGSRGLLWLEPLVEVGTAAG
CCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLSAADVPALFDAGWLEGGTHPSGVGLVDALPYLARQQRLTFARIGLTDPLSIDD
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHH
YLQHGGLAGLKNALALDGDAACETLIESGLRGRGGAAFPAGIKWRTVRQARAAQKYIVCN
HHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
ADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIVRA
CCCCCCCCCCCCEEECCCCHHHCCCHHEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHH
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLF
HHCCCCHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GQPTVINNVITLATAPVIFARGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPDHQWDVPLDYEAYTAIGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRHGDTSERQVTLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGKDAASDPVKAAA
HHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTRIYVPRDSSALALGADALAAAIVAEAERRGVTVELIRNGSRGLLWLEPLVEVGTAAG
CCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLSAADVPALFDAGWLEGGTHPSGVGLVDALPYLARQQRLTFARIGLTDPLSIDD
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHH
YLQHGGLAGLKNALALDGDAACETLIESGLRGRGGAAFPAGIKWRTVRQARAAQKYIVCN
HHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
ADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIVRA
CCCCCCCCCCCCEEECCCCHHHCCCHHEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHH
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLF
HHCCCCHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GQPTVINNVITLATAPVIFARGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPDHQWDVPLDYEAYTAIGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRHGDTSERQVTLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGKDAASDPVKAAA
HHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]