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The map label for this gene is nac [H]

Identifier: 116688958

GI number: 116688958

Start: 1043224

End: 1044144

Strand: Reverse

Name: nac [H]

Synonym: Bcen2424_0935

Alternate gene names: 116688958

Gene position: 1044144-1043224 (Counterclockwise)

Preceding gene: 116688962

Following gene: 116688957

Centisome position: 29.97

GC content: 69.38

Gene sequence:

>921_bases
ATGGACTTGAGACAGTTGCGCTATTTCGTGAAGGTCGTCGAATGCGGCAACGTCACGCACGCGAGCGAGGCGCTGCACGT
CGCGCAGCCGGCGGTGAGCCAGCAGATGCGCAATCTCGAGCAGGATCTCGGGATGCAACTGCTCGAACGAAGCGTGCGCG
GCGTCGCGCCGACCGCCGCCGGCCGCACGCTGTACCAGCACGCACTCGAACTGCTGCGCCAGGCCGACGGCACGCGCGAG
CTGCTGCGCCGCGACGCCGACACGCCGCAGGGCCGCGTGACCGTCGGCATGCCGTCGAGCACCGCGCGCGTGCTGGCCAT
TCCGCTCGCCCGCGCCGTGCGCGACCGCTATCCGGGCATCATGCTGGAGCTGATCGAAGCGCCGAGCGCCGACCTCGACA
CGCTGCTCGAACGCGCGCGGCTCGATCTCGCGATCGTCGTCGATGCGGTCGACACGCGCGGCATCGCGATCCACCGGTTG
CTGACGGAAACGCTGTACCTGATCACGTGGCCCGGATTCCCGGTGCCGGACGCGCCCGTGCCGCTCGACGCGATCGCGCG
GATGCCGCTCGTACTGCCGAGCGCGCCGAACACGATCCGCAACCGCGTCGACTGGGCGATGCGCGAGGCCGGGCTGTCGT
ACGAGATCAGCTTCGAGGCGAGTTCGACCGGGCTGCTGTTCGCGGCCGTGATGGCGCAGCTCGGTGTGACGATCCTGCCG
TGGACGGCCGCGCACGTCGAGATCGAGGAACGCAAGCTCAAGCTGTCGACGGTCGCGCACCGGCTGTTCGCGCGCGACCT
GTCGCTGTGCTGGCACGACACCGCGCTGGTCAGCAACGCGGTGCAGAAGGTGAAGTCCGAAATCGTCCGGCTGTTCAATA
CGCTCGGGCAGCGGCCGGAATGGGCGGCCGCCGGGCATTGA

Upstream 100 bases:

>100_bases
GCTATCGATCGCTGAAAAATATGATATTCATCTTGCTCGATCGGATTGGCTTATGGCTCGAATCGGGCGTGATTCGGGCT
TGAATCGGGAGACGGCGGCG

Downstream 100 bases:

>100_bases
CAATGGCCGCCGGCACCCCTTAACGTAGCTCCATCCTAACCATCACCGGTTCAGCTTAAGAGGTTGTCGGCCCGCTCGTG
CTCGACACCTCCGCCTTCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERARLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFPVPDAPVPLDAIARMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKSEIVRLFNTLGQRPEWAAAGH

Sequences:

>Translated_306_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERARLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFPVPDAPVPLDAIARMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKSEIVRLFNTLGQRPEWAAAGH
>Mature_306_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERARLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFPVPDAPVPLDAIARMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKSEIVRLFNTLGQRPEWAAAGH

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=39.1836734693878, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI157672245, Length=238, Percent_Identity=31.9327731092437, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=27.8688524590164, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=26.5017667844523, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=27.3809523809524, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=171, Percent_Identity=35.0877192982456, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=262, Percent_Identity=27.8625954198473, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788748, Length=241, Percent_Identity=28.6307053941909, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788296, Length=254, Percent_Identity=26.3779527559055, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789639, Length=159, Percent_Identity=31.4465408805031, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=26.3473053892216, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693193, Length=119, Percent_Identity=32.7731092436975, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082132, Length=250, Percent_Identity=25.6, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788626, Length=242, Percent_Identity=30.1652892561983, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=23.7154150197628, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=28.8461538461538, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=251, Percent_Identity=26.2948207171315, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33810; Mature: 33810

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GRTLYQHALELLRQADGTRELLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGI
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
MLELIEAPSADLDTLLERARLDLAIVVDAVDTRGIAIHRLLTETLYLITWPGFPVPDAPV
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCC
PLDAIARMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
CHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEC
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKSEIVRLFNTLGQRPE
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAAAGH
CCCCCC
>Mature Secondary Structure
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GRTLYQHALELLRQADGTRELLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGI
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
MLELIEAPSADLDTLLERARLDLAIVVDAVDTRGIAIHRLLTETLYLITWPGFPVPDAPV
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCC
PLDAIARMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
CHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEC
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKSEIVRLFNTLGQRPE
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAAAGH
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]