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The map label for this gene is pyrC' [H]

Identifier: 116688891

GI number: 116688891

Start: 971549

End: 972826

Strand: Direct

Name: pyrC' [H]

Synonym: Bcen2424_0868

Alternate gene names: 116688891

Gene position: 971549-972826 (Clockwise)

Preceding gene: 116688890

Following gene: 116688892

Centisome position: 27.89

GC content: 70.5

Gene sequence:

>1278_bases
ATGAAGATTCATATCAAAGGCGGCACGCTGATCGACCCGGTCGCCGGCACCGAAGGGCAGGCCGACGTATTCGTCGCGGA
CGGCAAGATCGCCGCGCTGGGCAGCGCGCCCGCCGGTTTCAACGCGGACCAGACGATCGACGCGTCGGGCCTGATCGTCG
CACCCGGCCTCGTCGACCTGTGCGCGCGGCTGCGCGAGCCGGGCTACGAACACAAGGCGACGCTCGCTTCCGAGATGGCC
GCGGCCGTCGCGGGCGGCGTCACGACCCTCGTGTGCCCGCCGGACACCGATCCCGTGCTCGACGAGCCGGGCCTCGTCGA
AATGCTCAAGTTCCGCGCACGCAATCTGCACCAGGCGAACGTGCACCCCCTTGGCGCGCTGACGGTCGGCCTCAAGGGCG
AGGTGATCACCGAGATGGTCGCGCTGACCGAATCCGGCTGCGTCGGTTTCACGCATGCCAACGTGCCGGTGCGCGACACG
CAGGTGCTGCTGCGCGCGCTGCAGTACGCGAGCACCTACGGCTACACGACGTGGCTGCGTCCGCTCGACGCGTTCATCGG
CCGCGGCGGCGTGGCCGCGAGCGGTGCGCTCGCGTCGCGGCTCGGGCTGTCCGGCGTGCCGGTCGCGGCCGAGACGATCG
CACTCCACACGATTTTCGAACTGATGCGCGTGACGGGCGCACGCGTGCACCTCGCGCGGCTGTCGTCGGCGGCCGGGCTC
GCGCTCGTGCGTGAAGCGAAGGCCGAGGGGCTGCCCGTGACCTGCGACGTCGGCGTGAACCACGTCCACCTGATCGACGT
CGACATCGGCTACTTCGACTCGCAGTTCCGGCTCGATCCGCCTCTGCGCAGCGAGCGCGATCGCGAAGCGATTCGCGTGG
CGCTCGCCGACGGCACGATCGATGCGATCTGCTCGGATCACACGCCGGTCGACGACGACGAGAAGCTGTTGCCGTTCGGT
GAAGCGACGCCGGGTGCGACGGGGCTCGAACTGCTGCTGTCGCTGACGCTGAAGTGGGCGGACGAGACGGGCACGCCGCT
CGCGCAGGCGCTGCGCCGCATCACGTCCGCGCCGGCGGACGTGCTGCAACTGCCGGCGGGGCGCCTCGCCGAAGGCGGCG
CGGCCGACCTGTGCGTGTTCGATCCGCGTGCGCATTGGCGCGTCGAGCCGCGTGCGCTGAAGAGCCAGGGCCACAACTCG
CCGTTCCTCGGCTACGAACTGCCGGCCACCGTGCGTACCACGCTCGTGGCCGGACGGGTCGCATTCGAGCGCCACTGA

Upstream 100 bases:

>100_bases
ATGGCGGTGATGGGCATCGTCGCCGGCAACAGCGACTGAGCCCGCTTTCGCGCCTCCTCGTTCAGGCATTCAACGCAATC
AACGCATTCACAGACAGCGC

Downstream 100 bases:

>100_bases
ACCACGCGGAACCTTCGCCATGATCGCCTTTCGCAAGCTGCGTCTCGCCTTTCACCTGTTGCGCGGCATGGTGATCGTCG
CGCTGCGTTTTTCGCACGTC

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKIHIKGGTLIDPVAGTEGQADVFVADGKIAALGSAPAGFNADQTIDASGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYGYTTWLRPLDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSAAGL
ALVREAKAEGLPVTCDVGVNHVHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETGTPLAQALRRITSAPADVLQLPAGRLAEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPATVRTTLVAGRVAFERH

Sequences:

>Translated_425_residues
MKIHIKGGTLIDPVAGTEGQADVFVADGKIAALGSAPAGFNADQTIDASGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYGYTTWLRPLDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSAAGL
ALVREAKAEGLPVTCDVGVNHVHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETGTPLAQALRRITSAPADVLQLPAGRLAEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPATVRTTLVAGRVAFERH
>Mature_425_residues
MKIHIKGGTLIDPVAGTEGQADVFVADGKIAALGSAPAGFNADQTIDASGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYGYTTWLRPLDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSAAGL
ALVREAKAEGLPVTCDVGVNHVHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETGTPLAQALRRITSAPADVLQLPAGRLAEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPATVRTTLVAGRVAFERH

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=400, Percent_Identity=27, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786722, Length=453, Percent_Identity=28.0353200883002, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082175, Length=441, Percent_Identity=25.6235827664399, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193204318, Length=404, Percent_Identity=25, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322218, Length=433, Percent_Identity=24.2494226327945, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24642586, Length=393, Percent_Identity=26.2086513994911, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18859883, Length=212, Percent_Identity=30.6603773584906, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 44744; Mature: 44744

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHIKGGTLIDPVAGTEGQADVFVADGKIAALGSAPAGFNADQTIDASGLIVAPGLVDL
CEEEECCCCEECCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCEECCCCCEECCCHHHH
CARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQAN
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
VHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDTQVLLRALQYASTYGYTTWLR
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
PLDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSAAGL
HHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCH
ALVREAKAEGLPVTCDVGVNHVHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTI
HHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCE
DAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTLKWADETGTPLAQALRRITSAPAD
EHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
VLQLPAGRLAEGGAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPATVRTTLVAGRV
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCEECCHHHHHHHHHHHH
AFERH
HCCCC
>Mature Secondary Structure
MKIHIKGGTLIDPVAGTEGQADVFVADGKIAALGSAPAGFNADQTIDASGLIVAPGLVDL
CEEEECCCCEECCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCEECCCCCEECCCHHHH
CARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKFRARNLHQAN
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
VHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDTQVLLRALQYASTYGYTTWLR
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
PLDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSAAGL
HHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCH
ALVREAKAEGLPVTCDVGVNHVHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTI
HHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCE
DAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTLKWADETGTPLAQALRRITSAPAD
EHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
VLQLPAGRLAEGGAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPATVRTTLVAGRV
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCEECCHHHHHHHHHHHH
AFERH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]