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The map label for this gene is tolB [H]

Identifier: 116688823

GI number: 116688823

Start: 901955

End: 903250

Strand: Reverse

Name: tolB [H]

Synonym: Bcen2424_0800

Alternate gene names: 116688823

Gene position: 903250-901955 (Counterclockwise)

Preceding gene: 116688824

Following gene: 116688822

Centisome position: 25.93

GC content: 65.12

Gene sequence:

>1296_bases
ATGAGTTTGATGACAAAGCTAGGTTTCAGGGCACTCGTGGCCTCGTGTCTGATCACGGCGGGCAGCGCCGCTAACGCCCA
GGTCAACGTGCTGATCACCGGTGTCGGGTCGACCCAGTTCCCCATTGCCACCGCGAACTTCACGAACGAGGCGAATCTGC
CGCAGCAGGTCACGTCGATCGTGCGCGCCGACCTCGCCCGCAGCGGCAAATTCACCAACATCGACGCGGGCAGCACGCCC
GTGCCCGAGACCGCCTCGGTCGATCTCGGCGCATGGAAGGCCAAGGGCGCGAATGCGTTCGTCGCCGGCAGCGTGAACCG
CGACGCGAACGGTCAGTACAAGGTCAACTTCATCCTGTACGACACCGTGAAGCAGCAAAGCCTCGGCGGCCTGTCGCTGA
CGGCCACCGACACCACGCTGCGCACGGCCGGCCACAAGATCGCCGACTACATCTATCAGAAGCTGCTCGGCGTGCGCGGC
GTGTTCGCCACGCGCCTGTCGTACGTGATCAAGACCGGCAACCGCTACCAGCTGCAGATTTCCGATTCGGATGGCCAGAA
CGCGCGCATCGCGCTGTCGAGCACCGAGCCGATCATCTCGCCGGCCTGGTCGCCGAGCGGCACGAAGGTCGCGTACGTAT
CGTTCGAGCGCAAGAAGCCGATCGTCTACATCCACGACCTGCCGACCGGTCGCCGCTACATGGTCTCCGACCAGAAGGGC
AACAACAGCGCACCGGCGTGGTCGCCGGACAGCAACACGCTGGCCGTCGCGCTGTCGCTGACGGGCAATACGCAAATCTA
TACGGTCAACGCGAACGGCGGCGGCCTGCGCCGTCTCACGCAGAGCAGCTCGATCGACACCGAACCGTTCTACTCGCCGG
ACGGTCGCTGGATCTACTTCACGAGCGATCGTGGCGGTGCGCCGCAAATCTACCGGATGCCCGCGCAGGGTGAAAGCGCC
GGCGCCGCGCAGCGCGTGACCTTCACCGGCAGCTACAACACGAGCCCGCGCGTGAGCCCGGACGGCAAGCTGCTCGCTTA
CATCTCCCGCACGGGTGGGGGCTTCAAGCTGTACGTTCAGGATCTGCAGACTGGCGCGGCGAACGCCATCACGAATACCA
ATCGCGACGAATCGCCGAGCTTCGCGGCAAACGGCCAGTACGTTCTGTACGCTACCCAGTCGGGTGGTCGCAACGTTCTG
GCTGCAGTGCCCTCCGACGGCAGCGCGCCGCCGCAGATCTTGTCCGTCCAGGGCGGCTCCGTTCGCGAGCCGTCGTGGGG
GCCCTTCATGCAATGA

Upstream 100 bases:

>100_bases
CGCTTACACTCCGGGCGTCGCTGTCAGATGGGAACGAATCGGGTATTTTTACTGTCTCTGCCGTTGCGCAGCGATCCAAG
CCATACGGGAAGCAAGAAGC

Downstream 100 bases:

>100_bases
CCACAAGGAGAGTAACCATGATGTCGAATAAAGCTCGTCTGGCCCTGGCCGTGATGATGATCAGCGCGCTCGCAGCGTGC
AAGTCGGGCGTGAAGCTCGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFTNEANLPQQVTSIVRADLARSGKFTNIDAGSTP
VPETASVDLGAWKAKGANAFVAGSVNRDANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNANGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYVLYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Sequences:

>Translated_431_residues
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFTNEANLPQQVTSIVRADLARSGKFTNIDAGSTP
VPETASVDLGAWKAKGANAFVAGSVNRDANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNANGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYVLYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ
>Mature_430_residues
SLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFTNEANLPQQVTSIVRADLARSGKFTNIDAGSTPV
PETASVDLGAWKAKGANAFVAGSVNRDANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGV
FATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKGN
NSAPAWSPDSNTLAVALSLTGNTQIYTVNANGGGLRRLTQSSSIDTEPFYSPDGRWIYFTSDRGGAPQIYRMPAQGESAG
AAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQDLQTGAANAITNTNRDESPSFAANGQYVLYATQSGGRNVLA
AVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=439, Percent_Identity=37.5854214123007, Blast_Score=257, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 45756; Mature: 45624

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFTNEANLPQQVTSI
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPETASVDLGAWKAKGANAFVAGSVNRDANGQYKVNFILY
HHHHHHHCCCEEECCCCCCCCCCCCEECCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNANGGGLRRLTQSSSIDTEPFYSPDGRWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQTGAANAITNTNRDESPSFAANGQYVLYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC
>Mature Secondary Structure 
SLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFTNEANLPQQVTSI
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPETASVDLGAWKAKGANAFVAGSVNRDANGQYKVNFILY
HHHHHHHCCCEEECCCCCCCCCCCCEECCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPAWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYMVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNANGGGLRRLTQSSSIDTEPFYSPDGRWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRVSPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQTGAANAITNTNRDESPSFAANGQYVLYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA