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The map label for this gene is pcnB [H]

Identifier: 116688781

GI number: 116688781

Start: 849061

End: 850617

Strand: Reverse

Name: pcnB [H]

Synonym: Bcen2424_0758

Alternate gene names: 116688781

Gene position: 850617-849061 (Counterclockwise)

Preceding gene: 116688782

Following gene: 116688780

Centisome position: 24.42

GC content: 69.3

Gene sequence:

>1557_bases
GTGATCAAAAAATTCATTCGCAAGCTCCTCGGCCAGGACGAAACCGAGCAAACGCATCCCGCAGCGGCCCCGGCCGACGA
AGCGGCCCCTGCTGCCCCGCGCGCCCCGAAGGGCGCCCGCGGCGGCGGCGCGAAGAGACCGCGCAGCAACCATGAGCCGA
CCGTCGTGCCGGCCAGCGTGCACGGCATCAACCCGGCGCTGATCTCGAAGAACGCGGTGCGCGTGACCGACACGCTGCAG
CAGGCGGGTTTCCGCGCGTTCATCGTCGGCGGCGCGGTGCGCGACCTGCTGCTCGGCATCGCGCCGAAGGATTTCGACGT
CGCGACCGACGCGACGCCGACCGAGGTGCAGCGCCTGTTCCGCCGTGCGCGCCTGATCGGCCGCCGCTTCCAGATCGTCC
ACGTGCAGTTCGGCCAGGACCTGATCGAAGTGTCGACGTTCCGCGCGCTGGTCGACGCGCCGCCCGAGGCGGCCGCGGCC
ACGGCCGAGCCGCCGCGGCGCCTGAAGCGCGACGAACTCGATCGCCGCACGCATGCGGTCGACGCGAGCGGCCGCGTGCT
GCGCGACAACGTGTGGGGCGAGCAGCACGAGGACGCCGCGCGCCGCGACTTCACGATCAACGCGATGTACTACGACCCGT
CGACGCAGACGGTGCTCGACTACCACGACGGGATGGCCGACGTACGCGCCCGCCTGTTGCGGATGATCGGCGATCCGGCC
ACGCGCTTTCGCGAGGATCCGGTGCGGATGCTGCGCGTCGTGCGCTTCGCGGCGAAGCTCGGCTTCGAGATCGAGCCGCA
TACGCGCGAGCCGATCCACGCGCTCGCCGACCTGATCAACAACGTGCCGGCCGCGCGCCTGTTCGACGAAATGCTGAAGC
TGCTGCTGTCGGGCCACGCGCTCGCGTGCCTGCAGCGGCTGCGCAAGGAAGGCCTGCATCACGGGCTGCTGCCGCTGCTC
GACGTCGTGCTCGAACAGCCGCAGGGCGAGAAATTTATCACGCTCGCGCTGAACAACACCGACGCGCGCGTGCGCGCCGG
CAAGCCGGTGTCGCCGGGCTTCCTGTTCGCGACGCTGCTGTGGCACGACATGCGCCAGCGCTTCGAGCAATACACGGCCG
CGGGCGAAATCCCGGTGCCGGCGCTCCATCGCGCGATGGACGACGTGCTCGACATGCAGACCGAGAAACTCGCGATCCAC
AAGCGTTACTCGGCCGACATGCGCGAGATCTGGGGCCTGCAGCTGCGCCTCGAGAAACGCTCGGGCCGCAGCGCGATGCG
GCTGCTGGAACACCAAAGGTTTAGAGCGGGGTATGATTTCCTCCTGTTACGCTGCGAATCCGGCGAGCTCGATGCGGAAG
TCGGACAGTGGTGGACGGATTTCATCGAAGGCGACGCGGCCGCTCGCGAGGCATTGCTCACGCAGGGCGGCTCGAAGGAA
AAATCGCCCCGCAAGCGGCGCCGCCGCGGCGGTGCGCGAAACCGCCAGCAGGGTGAAGGTGCCGCCGAGCAGGCGCCGGA
CACCGCAGGCGGTACCGCGAGCGGTTCCGACGACTGA

Upstream 100 bases:

>100_bases
TCGAGAAAGTCACCGACCCGATCGCGACCAACCCTGACGACACGCTGCGCGCGCATGCAAGCAACCGCGGCTGGCGTATC
CTCGATCTCTTCTGAACGTC

Downstream 100 bases:

>100_bases
CGCGCGCGCCGGCGAACGACGTAGGAAGTGATGCCATGACGGTTGCATATATCGGACTGGGGGCGAATCTCGGCGATGCG
CGCCAGACCCTGAAGGACGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MIKKFIRKLLGQDETEQTHPAAAPADEAAPAAPRAPKGARGGGAKRPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQDLIEVSTFRALVDAPPEAAAA
TAEPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPA
TRFREDPVRMLRVVRFAAKLGFEIEPHTREPIHALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLL
DVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAAGEIPVPALHRAMDDVLDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGSKE
KSPRKRRRRGGARNRQQGEGAAEQAPDTAGGTASGSDD

Sequences:

>Translated_518_residues
MIKKFIRKLLGQDETEQTHPAAAPADEAAPAAPRAPKGARGGGAKRPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQDLIEVSTFRALVDAPPEAAAA
TAEPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPA
TRFREDPVRMLRVVRFAAKLGFEIEPHTREPIHALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLL
DVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAAGEIPVPALHRAMDDVLDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGSKE
KSPRKRRRRGGARNRQQGEGAAEQAPDTAGGTASGSDD
>Mature_518_residues
MIKKFIRKLLGQDETEQTHPAAAPADEAAPAAPRAPKGARGGGAKRPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQDLIEVSTFRALVDAPPEAAAA
TAEPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPA
TRFREDPVRMLRVVRFAAKLGFEIEPHTREPIHALADLINNVPAARLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLL
DVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAAGEIPVPALHRAMDDVLDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGSKE
KSPRKRRRRGGARNRQQGEGAAEQAPDTAGGTASGSDD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=245, Percent_Identity=27.3469387755102, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI87081691, Length=467, Percent_Identity=41.541755888651, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1789436, Length=258, Percent_Identity=31.3953488372093, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57677; Mature: 57677

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKFIRKLLGQDETEQTHPAAAPADEAAPAAPRAPKGARGGGAKRPRSNHEPTVVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
HGINPALISKNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQDLIEVSTFRALVDAPPEAAAATAEPPRRLKRDELDRRTHAV
HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHC
DASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPA
CCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHH
TRFREDPVRMLRVVRFAAKLGFEIEPHTREPIHALADLINNVPAARLFDEMLKLLLSGHA
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
LACLQRLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
WHDMRQRFEQYTAAGEIPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
SGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGSKE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
KSPRKRRRRGGARNRQQGEGAAEQAPDTAGGTASGSDD
CCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKFIRKLLGQDETEQTHPAAAPADEAAPAAPRAPKGARGGGAKRPRSNHEPTVVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
HGINPALISKNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQDLIEVSTFRALVDAPPEAAAATAEPPRRLKRDELDRRTHAV
HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHC
DASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPA
CCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHH
TRFREDPVRMLRVVRFAAKLGFEIEPHTREPIHALADLINNVPAARLFDEMLKLLLSGHA
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
LACLQRLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
WHDMRQRFEQYTAAGEIPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
SGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGSKE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
KSPRKRRRRGGARNRQQGEGAAEQAPDTAGGTASGSDD
CCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]