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The map label for this gene is araF [H]

Identifier: 116688644

GI number: 116688644

Start: 692513

End: 693508

Strand: Direct

Name: araF [H]

Synonym: Bcen2424_0621

Alternate gene names: 116688644

Gene position: 692513-693508 (Clockwise)

Preceding gene: 116688643

Following gene: 116688645

Centisome position: 19.88

GC content: 66.37

Gene sequence:

>996_bases
ATGAAACGCAGAACGTTCGTAACGCTGGCCGCCGCGGCGGCGGTGGTGATGGGCAGCCCGGTCGCGCACGCGGCCGACCC
GGTCAAGATCGGCTTCCTGGTGAAGCAGCCGGAAGAGCCGTGGTTCCAGGACGAGTGGAAATTCGCCGAGATCGCCGCGA
AGCAGAAGGGCTTCACGCTCGTGAAGATCGGCGCGCCGTCCGGCGAGAAGGTGATGAGCGCGATCGACAACCTGTCCGCG
CAGAAGGCGCAGGGCTTCATCATCTGCACGCCCGACGTGAAACTCGGGCCGGGCATCGTTGCGAAGGCGAAGTCGCACAA
CCTGAAGATGATGACGGTCGACGACCGCCTCGTCGACGGCGCAGGCAAGCCGATCGAGTCGGTGCCGCACATGGGGATTT
CCGCGTACAACATCGGCAAGCAGGTCGGCGACGGCATCGCGGCCGAGATCAAGAAGCGCGGCTGGGACATGAAGGACGTC
GGCGCGATCGACATCACCTACGAGCAACTGCCGACCGCGCACGACCGCACGAGCGGCGCGACCGACGCGCTGGTCGCCGC
CGGCTTCCCGAAGGCGAACGTGATCGCGGCGCCGCAGGCGAAGACCGATACGGAGAACGCGTTCAACGCGGCGAACATCG
CGCTCACGAAGAACCCGCAGTTCAAGCACTGGGTCGCGTACGGCCTGAACGACGAGGCCGTGCTCGGCGCGGTGCGCGCG
GCGGAAGGGCGCGGCTTCAAGGCCGACAACATGATCGGCATCGGCATCGGCGGTTCCGATTCGGCGTTGAACGAGTTCAA
GAAGCCGCAGCCGACGGGCTTCTACGGCACCGTGATCATCAGCCCGAAGCGCCACGGCGAGGAAACCTCGGACCTGATGT
ACACGTGGATCACGCAGGGCAAGGCGCCGCCGCCGCTGACGCTGACGACCGGCATGCTGGCGACGCGCGACAACGTGGCG
AAGGTGCGCGAGGAGATGGGGCTCGCGTCGAAGTAA

Upstream 100 bases:

>100_bases
TAACGATTCGAAAGACCGCACGTGCGACGGCCGCGCGCACGCTGCGTCGAAACCAACAAGCGGCTGCATCCTGTTCGATG
AAAACAAGGAGACACTGGAG

Downstream 100 bases:

>100_bases
GCGGCCCGCCGGCTGCCGCGGCGATGCGGCGGCCGGCGGTCGATCGATCTGGAAGTGGGGAGACGACGTGTCAGCGGCAC
TGCGTTTTGACAATATCGGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKQKGFTLVKIGAPSGEKVMSAIDNLSA
QKAQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWDMKDV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALNEFKKPQPTGFYGTVIISPKRHGEETSDLMYTWITQGKAPPPLTLTTGMLATRDNVA
KVREEMGLASK

Sequences:

>Translated_331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKQKGFTLVKIGAPSGEKVMSAIDNLSA
QKAQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWDMKDV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALNEFKKPQPTGFYGTVIISPKRHGEETSDLMYTWITQGKAPPPLTLTTGMLATRDNVA
KVREEMGLASK
>Mature_331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKQKGFTLVKIGAPSGEKVMSAIDNLSA
QKAQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWDMKDV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALNEFKKPQPTGFYGTVIISPKRHGEETSDLMYTWITQGKAPPPLTLTTGMLATRDNVA
KVREEMGLASK

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=332, Percent_Identity=47.289156626506, Blast_Score=314, Evalue=4e-87,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35213; Mature: 35213

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKQKGFTL
CCCCEEEHHHHHHHHHHCCCCHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEE
VKIGAPSGEKVMSAIDNLSAQKAQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDG
EEEECCCCHHHHHHHHHCCHHHHCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCEECC
AGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWDMKDVGAIDITYEQLPTAHDRTSGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKADNMIGIGIGGSDSALNEFKKPQPTGFYGTVIISPKRHGEETSDLMYTWITQG
CCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEEEEEECC
KAPPPLTLTTGMLATRDNVAKVREEMGLASK
CCCCCEEEECCCEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKQKGFTL
CCCCEEEHHHHHHHHHHCCCCHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEE
VKIGAPSGEKVMSAIDNLSAQKAQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDG
EEEECCCCHHHHHHHHHCCHHHHCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCEECC
AGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWDMKDVGAIDITYEQLPTAHDRTSGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKADNMIGIGIGGSDSALNEFKKPQPTGFYGTVIISPKRHGEETSDLMYTWITQG
CCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEEEEEECC
KAPPPLTLTTGMLATRDNVAKVREEMGLASK
CCCCCEEEECCCEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]