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The map label for this gene is proB [H]

Identifier: 116688608

GI number: 116688608

Start: 655818

End: 656936

Strand: Direct

Name: proB [H]

Synonym: Bcen2424_0584

Alternate gene names: 116688608

Gene position: 655818-656936 (Clockwise)

Preceding gene: 116688607

Following gene: 116688611

Centisome position: 18.82

GC content: 67.92

Gene sequence:

>1119_bases
ATGCGTTCGATCATCGCGGATTCGAAGCGATTGGTGGTGAAAGTGGGCTCGAGCCTCGTGACCAACGACGGCAAGGGGCT
CGATCATGCTGCAATCGGCCGCTGGGCAGCCCAGATCGCGGCACTGCGTGCGCAGGGCAAGGAAGTCGTGCTCGTCAGTT
CGGGGGCGATTGCCGAAGGCATGCAGCGGCTCGGCTGGAGCAAGCGGCCGCGGGAAATCGACGAGCTGCAGGCCGCCGCC
GCGGTCGGGCAGATGGGGCTCGCGCAAGTCTACGAGAGCCGCTTCACCGAGCACGGCATCCGCACCGCGCAGATCCTGCT
CACGCACGCCGATCTGGCGGATCGCGAGCGCTACCTGAACGCGCGTTCGACGCTGCTCACGCTGCTGCGGCTCGGCGTCG
TGCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAAATCAAGTTCGGCGACAACGACACGCTCGGCGCGCTCGTC
GCGAACCTGATCGAAGGCGATGCGCTGATCATCCTCACCGACCAGTCGGGGCTGTTCACGGCCGATCCGCGCAAGGACCC
GAACGCGACGCTCGTCGGCGAGGCCAACGCGGGCGCACCGGAGCTCGAGGCGATGGCGGGCGGGGCCGGTTCGAGTCTCG
GTCGCGGCGGGATGCTGACGAAGATCCTCGCGGCGAAGCGCGCGGCACACAGCGGCGCGAACACCGTGATCGCGAGTGGC
CGCGAGGCCGACGTGCTCGTCCGTCTGGCGGCTGGCGAGGCGATCGGCACGCAACTGATCGCACGTACCGCGCGGATGGC
GGCACGCAAGCAATGGATGGCCGACCATCTGCAGGTGCGCGGCCACGTCGTGATCGATGCGGGCGCGGTCGAGAAGCTCA
CGGCCGGCGGCAAGAGCCTGCTGCCGATCGGCGTGATCGACGTGCAGGGCGCGTTCGCGCGCGGCGAAGTGATCGCCTGC
GTCGGCCCGGACGGGCGCGAGGTGGCGCGCGGGCTCACGAACTACAGCAGCGCGGAAACGAAGCTGATTCACCGCAAGCC
GAGCGGCGAGATCGAGACCGTGCTTGGCTACATGCTGGAGCCCGAACTGATTCATCGCGACAACCTCGTGCTGGTGTAA

Upstream 100 bases:

>100_bases
ACGACGCCTGAACGTTTGCTAACGCGCCGGGCGTGCCGCCCGGCGTTGGCGTTCAATCGCGCCCGGCCGGGCAGGCATCA
GATACAGGAGACAGTGCAGG

Downstream 100 bases:

>100_bases
TCGCCGGCCGGGTTTCGTCGTTCAAATGAAAAAGCCCGCGTGATGCGGGCTTTTTTGTGCGGCGGACGATGCGGGCGGCC
CGCATCGCGTGCCGTCAGCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVGEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
READVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVGEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
READVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVGEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
READVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=362, Percent_Identity=33.4254143646409, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI62912457, Length=360, Percent_Identity=33.6111111111111, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.4426229508197, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=35.5871886120996, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=35.5871886120996, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=36.6666666666667, Blast_Score=215, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.6086956521739, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21357643, Length=350, Percent_Identity=31.7142857142857, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39213; Mature: 39213

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVGEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
READVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCC
LPIGVIDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVGEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
READVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCC
LPIGVIDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA