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The map label for this gene is yoaE [H]

Identifier: 116688569

GI number: 116688569

Start: 612016

End: 614091

Strand: Direct

Name: yoaE [H]

Synonym: Bcen2424_0545

Alternate gene names: 116688569

Gene position: 612016-614091 (Clockwise)

Preceding gene: 116688568

Following gene: 116688570

Centisome position: 17.57

GC content: 64.16

Gene sequence:

>2076_bases
ATGAACGCCGCCACCCAGGTAGCGCGGGCCGTTTGCCCGCATGATTGTCCGGACACGTGCGCAATGCGTGTGACCGTCGA
GAACGGCAAGGCAATCAAGGTCACCGGCGATCCCGACCATCCGCCGACGCAGGGCGTGTTGTGCACGAAAGTCAGTCGCT
ACGCGGATCGCGTTCATCATCCCGATCGCCTCACTGTCCCGCTCAAGCGCGTCGGCGCAAAAGGGGAAGGGCGCTTCGTG
CCGATCAGCTGGAGCGAGGCGTTCGACGAGATCGGACGTCGCCTCGGTGAAATCACCGCACGCGCGCCGGAAGCGATCGT
GCCGTACAGCTATGCGGGAACGATGGGGCTCGTGCAAGGCGAGGGTATTGCCCAGCGTTTCTTCCACAAGCTCGGCGCTT
CGCGGCTGGAGCGTGCGATCTGCGCGGCGGCCGGCGCAGCGGGGCTGCGATACACATACGGCGGCAGTGTCGGCATGCAC
CTCGAGTACTTCGAGGAAAGTGAACTGATTCTGATATGGGGCGCGAATCCGATTGCATCGAGCCTGCACTTCTGGACCCG
CGCCCAGGAAGCGAAGCGCCGTGGCGCGCGTCTGGTCGCGATCGACCCGTATCGTTCGCTGACTGCGGAGAAATGCCATC
AGCACATTGCATTGAAGCCCGGTACCGATGGCGCGTTTGCGCTCGGCATGATGCATGTGTTGATCACCGAAAACCTGCTC
GATCACGATTACATCGCCGACCATACGCTCGGCTTCGACGCGCTCAAGGCGCGCGCAATGTCCTATCCGCCGGAACGCGT
TGCGCAGATCTGCGGCATCGATGCGTCGGAGCTGGTCGATCTTGCGCGTCGCTACGGCGCTACGCGCAAGGCGTCGATTC
GCCTCAACTACGGTATGCAGCGTGTTCGCGGCGGCGGCAATGCGGTGCGTGCGATTGCCAGCCTGCCGGCACTGACGGGG
GCGTGGCGCGACCGGGCGGGCGGCCTGCTGCTCTCGTCGTCGGAATCCGCGCCGGTCAACCAGGCGGCGCTGCTGCGTCC
GGATCTGATGCCGGGTTGGCCGCATAAGCTGCCGCGCATCATCAACATGAACGCGATCGGCGACGCACTGCTGCACCCCG
GCGACGCGACGTTCGGGCCGAAAGTCGAAGCGGTGATCGTGTACAACTCGAATCCGGTAGCGGTCGCGCCCGATTCGTCG
AAGGTGGCCGCAGGTTTCGCACGCGATGATTTGTTCACCGTCGTTCTCGAGCACTTCAAGACAGATACGGTCGATTTCGC
CGACATTGTATTGCCGGCGACGACGCAGCTCGAGCATTTGGATGTCCATAAGTCGTACGGCCATACCTACGTGATGGCGA
ACCTGCCGGCGATTCCGCCGGTGGGTGACGCGCGGCCCAACACGGAGATCTTCCGGGGAATCGCGCGCAGCATGGGACTC
GACGAACCGGCGCTTTATCACAGCGACGAAGAGGTCGCTCGCGCGGCGCTTCGCTGGGACGATCCGGCGCTCGACAGCGA
CTGGGACACGCTGAAGCAGGCCGGCTGGCTGAAGCTCAAGCTGCCTGAGGCGCCGTTCGCGAATGGTGGTTTCCGCACGC
CCTCCGGCAAGTGCGAGTTTTACAGCCCCCGGCTCGAACAGATGGGCATGGACCCGGTCCCTGACTACCTGCCTCCGTTC
GAGTCGGCTGAAGCCGCGCCCGAGCTTGCCGCGCGCTATCCGCTGGCGATGATTTCGCCACCGGCCCGCCACTTCCTGAA
CAGCACATTCGTGAACGTCGACAGCCTGCGCTCGACGGAAGGCGAGCCGCACCTCGACATGCACCCGTCCGACGCCGATG
CGCGCGGCATCGCGGAGGGCGACACCGTGCGCATCTTCAATGACCGTGGCTCGATGCAGGCGCTCGCGCGCATTACCGAC
CGTGCGCGTGCCGGGCTCGTCGTCGGATTGTCGATCTGGTGGAAGAAGCTGTCGCCGGACGGCAGGAACGCGAACGAGGT
GACGAGTCAGGCGCTGACCGATTTGGGGAATTCGGCGACGTTTTACGATTGTCTTGTGGAAGTTGAGCGGATTTGA

Upstream 100 bases:

>100_bases
CTCGACCGCCGCGTAGATCATCCAAAATCGCTGCGAAATGAACGCTTTATTCGGCGCGTCCACGTAAAATTTGCGAACCC
CCATCTCTACATTCAAAACG

Downstream 100 bases:

>100_bases
TCGAATATCGCCGTATCATCAGACGAAATTGATGTGGAAGTTCGAAGTGGACATCTAACCCTGTTGTCTCGGGGCGGGGG
AGCCGTTACGATGCGTTTTA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MNAATQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRVGAKGEGRFV
PISWSEAFDEIGRRLGEITARAPEAIVPYSYAGTMGLVQGEGIAQRFFHKLGASRLERAICAAAGAAGLRYTYGGSVGMH
LEYFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARAMSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPALDSDWDTLKQAGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGMDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRSTEGEPHLDMHPSDADARGIAEGDTVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI

Sequences:

>Translated_691_residues
MNAATQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRVGAKGEGRFV
PISWSEAFDEIGRRLGEITARAPEAIVPYSYAGTMGLVQGEGIAQRFFHKLGASRLERAICAAAGAAGLRYTYGGSVGMH
LEYFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARAMSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPALDSDWDTLKQAGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGMDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRSTEGEPHLDMHPSDADARGIAEGDTVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI
>Mature_691_residues
MNAATQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRVGAKGEGRFV
PISWSEAFDEIGRRLGEITARAPEAIVPYSYAGTMGLVQGEGIAQRFFHKLGASRLERAICAAAGAAGLRYTYGGSVGMH
LEYFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARAMSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPALDSDWDTLKQAGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGMDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRSTEGEPHLDMHPSDADARGIAEGDTVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=765, Percent_Identity=28.6274509803922, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787870, Length=781, Percent_Identity=28.0409731113956, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI171474008, Length=761, Percent_Identity=27.5952693823916, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI3868721, Length=729, Percent_Identity=25.3772290809328, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI1787231, Length=733, Percent_Identity=25.5115961800819, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI87081994, Length=755, Percent_Identity=25.1655629139073, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI145693196, Length=725, Percent_Identity=27.0344827586207, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1788534, Length=348, Percent_Identity=21.8390804597701, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI3868720, Length=267, Percent_Identity=27.7153558052434, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1787778, Length=289, Percent_Identity=25.6055363321799, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI3868719, Length=232, Percent_Identity=26.7241379310345, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787741, Length=326, Percent_Identity=23.3128834355828, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75214; Mature: 75214

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAATQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRVGAKGEGRFVPISWSEAFDEIGRRLGEITARAPEAIVPYSYAGTMGLVQG
CCCEECCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EGIAQRFFHKLGASRLERAICAAAGAAGLRYTYGGSVGMHLEYFEESELILIWGANPIAS
CHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIADHTLGFDALKARAMSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQ
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCE
INMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSSKVAAGFARDDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHCCHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGDARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPALDSDWDTLKQAGWLKLKLPEAPFANGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEE
YSPRLEQMGMDPVPDYLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRSTE
ECCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEECCHHHHHHHHHHEEHHHHHCCC
GEPHLDMHPSDADARGIAEGDTVRIFNDRGSMQALARITDRARAGLVVGLSIWWKKLSPD
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GRNANEVTSQALTDLGNSATFYDCLVEVERI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNAATQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRVGAKGEGRFVPISWSEAFDEIGRRLGEITARAPEAIVPYSYAGTMGLVQG
CCCEECCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EGIAQRFFHKLGASRLERAICAAAGAAGLRYTYGGSVGMHLEYFEESELILIWGANPIAS
CHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIADHTLGFDALKARAMSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQ
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCE
INMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSSKVAAGFARDDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHCCHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGDARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPALDSDWDTLKQAGWLKLKLPEAPFANGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEE
YSPRLEQMGMDPVPDYLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRSTE
ECCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEECCHHHHHHHHHHEEHHHHHCCC
GEPHLDMHPSDADARGIAEGDTVRIFNDRGSMQALARITDRARAGLVVGLSIWWKKLSPD
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GRNANEVTSQALTDLGNSATFYDCLVEVERI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]