BacMap

Definition	Burkholderia cenocepacia HI2424 chromosome 1, complete sequence.
Accession	NC_008542
Length	3,483,902

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The map label for this gene is fadB1 [H]

Identifier: 116688568

GI number: 116688568

Start: 605087

End: 605863

Strand: Direct

Name: fadB1 [H]

Synonym: Bcen2424_0544

Alternate gene names: 116688568

Gene position: 605087-605863 (Clockwise)

Preceding gene: 116688562

Following gene: 116688569

Centisome position: 17.37

GC content: 64.09

Gene sequence:

>777_bases
ATGGCTTACGAGAACATCCAGGTGGAGACCCGGGGGCGTGTCGGTCTGGTGACGCTGAACCGTCCGAAGGCGCTGAATGC
GCTGAACGATGCGCTGATGGATGAGTTGGGCGCGGCACTGAAGGCGTTCGATGCGGACGACGATATCGGTGCGATCGTCG
TGACCGGGAGCGAGAAGGCGTTCGCGGCCGGCGCGGACATCGGCATGATGGCGACCTACTCCTATATGGATGTTTATCGG
GGCGACTACATCACGCACAACTGGGAGACGGTCCGCGAGATCCGCAAACCGATCATTGCCGCAGTTTCGGGCTTTGCGCT
GGGCGGCGGCTGCGAACTCGCGATGATGTGCGACATCATCTTCGCGGCGGACACGGCCAAGTTCGGTCAACCGGAAATCA
AGCTGGGCGTCATGCCGGGTGCGGGCGGCACGCAGCGTCTGCCGCGCGCGGTATCGAAGGCGAAGGCGATGGACATGTGC
CTGACCGCGCGCTTCATGGACGCCGCCGAGGCCGAGCGTGCCGGGCTCGTTTCGCGCGTGCTGCCGGCCGACAAGGTGCT
CGAGGAGGCGATCGCCGCCGCGACGACGATCGCCGAGTTTTCGCTGCCGGCGGTCATGATGGTCAAGGAGTCGGTGAACC
GCGCGTACGAGACGACGCTGGCTGAAGGCGTTCACTTCGAGCGCCGGCTGTTCCATTCGCTGTTCGCAACCGAAGACCAG
AAGGAAGGGATGGCGGCATTCGTCGAGAAGCGGAAGCCGGTGTTCAAGCATCGCTGA

Upstream 100 bases:

>100_bases
TTGGATCGATTAATTAACCGACCGGTTGGTCGGAGAATGGTAGCATCAAACTATTCGCATGTGCGAGGCGTTTCTCATTT
CATTGATCGAGGAGAAATAG

Downstream 100 bases:

>100_bases
TCGACTGGCGTCGGTTGGGGTCCGGTGGCTGGCCTGCATCGGTTATCGGCGTAATCGCCTTGACCCGTTCATCAAAAGCC
CCCGTGCGCGGCAGCGCACG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYENIQVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDDIGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITHNWETVREIRKPIIAAVSGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERAGLVSRVLPADKVLEEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAYENIQVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDDIGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITHNWETVREIRKPIIAAVSGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERAGLVSRVLPADKVLEEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AYENIQVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDDIGAIVVTGSEKAFAAGADIGMMATYSYMDVYRG
DYITHNWETVREIRKPIIAAVSGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCL
TARFMDAAEAERAGLVSRVLPADKVLEEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=56.3706563706564, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=35.7692307692308, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=32.7067669172932, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=30.2040816326531, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=34.3283582089552, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=210, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.1475409836066, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI37594469, Length=214, Percent_Identity=28.9719626168224, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI37594471, Length=214, Percent_Identity=28.9719626168224, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=28.0487804878049, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=28.0487804878049, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=26.1603375527426, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=246, Percent_Identity=26.0162601626016, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.9027237354086, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.9027237354086, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=24.609375, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=24.609375, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=27.0142180094787, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=27.0142180094787, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=161, Percent_Identity=31.055900621118, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=37.037037037037, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=26, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=46.2151394422311, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=32.9545454545455, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=31.6205533596838, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=29.2307692307692, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=31.6666666666667, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788682, Length=206, Percent_Identity=32.5242718446602, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=55.3846153846154, Blast_Score=293, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.9094650205761, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=34.251968503937, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.9113924050633, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.8181818181818, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=35.5191256830601, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=218, Percent_Identity=35.7798165137615, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=35.7798165137615, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=35.7798165137615, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.953125, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=29.2592592592593, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=218, Percent_Identity=30.2752293577982, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=218, Percent_Identity=30.2752293577982, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=183, Percent_Identity=34.4262295081967, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=263, Percent_Identity=24.3346007604563, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=177, Percent_Identity=29.3785310734463, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=28.2722513089005, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=57.7519379844961, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=57.7519379844961, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=36.5758754863813, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=30.8880308880309, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=32.3943661971831, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=242, Percent_Identity=26.8595041322314, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=27.6041666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921000, Length=185, Percent_Identity=33.5135135135135, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583077, Length=185, Percent_Identity=33.5135135135135, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583079, Length=185, Percent_Identity=33.5135135135135, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571729, Length=231, Percent_Identity=28.1385281385281, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571730, Length=231, Percent_Identity=28.1385281385281, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27964; Mature: 27832

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYENIQVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDDIGAIVVTGSEKA
CCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITHNWETVREIRKPIIAAVSGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPADKVLEEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYENIQVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDDIGAIVVTGSEKA
CCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITHNWETVREIRKPIIAAVSGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPADKVLEEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]