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The map label for this gene is gcvA [H]

Identifier: 116688543

GI number: 116688543

Start: 577552

End: 578469

Strand: Reverse

Name: gcvA [H]

Synonym: Bcen2424_0519

Alternate gene names: 116688543

Gene position: 578469-577552 (Counterclockwise)

Preceding gene: 116688548

Following gene: 116688542

Centisome position: 16.6

GC content: 72.55

Gene sequence:

>918_bases
ATGAATCGTTCCCGCCTGCCGCCGCTCAACGCATTGCGCGCGTTCGAAGCCGCCGCCCGCCACCTGTCGGTGAAGTCGGC
GGCCGAGGAGCTGTCGGTCACGCCCGGCGCGGTGAGCCAGATGATCCGCACGCTCGAGACGCATCTCGGCGTGCAGCTGT
TCGAGCGCGTGAACCGCGGCATCCTGCTGACGGCCGCCGGCCGCGACTACCTGCCGCCCGTGCGCAACGCGTTCCGGCAG
ATCGCCGATGCGTCGCAGCGCGTGTCGGGCGCCGCCGACAGCGGCGTGCTGACGGTGAGCGTCACGCCGTTCTTCGCGTC
CGCGTGGCTGGTCCCACGCCTCGCGCAGTTCCGGCAGGCGTGCCCGGACGTCGACCTGCAGGTCCTCACGAGCCACGCGC
TCGCGGATTTCGCGCGCGACGGCGTCGACGTCGCGATCCGCCACGGCCTCGGGCGCTACATCGGGCTGTGCAGCGAACGC
CTGCTGACGGTGGAAATCGTCGCGCTCGCGTCGCCGGGCCTCGTCGCGCGGCTCGGCATGCCGGCGACGCCTGCCGAGCT
CGTCGGCTGGCCGCAGTTGCACGACGCCGAACGCAAGGGCTGGCACATCTGGTTCGGCGCGCAGCGGATCGACGATTTCG
GCCCGCCGCGCGGCCCCGCGTTCGACGATTCGGGCCTGCTGCTGCAGGCGATCGTCGCCGGCCAGGGCGCCGGGCTGCTG
CCGGCCGCGATGGTGCGCGGCGAGCTGGCGAGCGGCCGGCTCGTGCAACTTGCCGACGTCGCATGGCTCGACGATTTCGC
GTACTACCTCGTCTATCCGCCGCATCACGCGGAACGGCCGAAGGTTGCCGCGTTCCGCCGCTGGATTCTCGATGCCGCGC
AGGCCGACGGCGCGGCGTCGATGACCGGCGCGGCGTAG

Upstream 100 bases:

>100_bases
CAGGCAGCCGCGAAACAGGCGATCCGTTGCGTCAACGGTACGCGCGCATACGCGTCGTCTCAAACGATATTTCGTCGACC
TTTCGCTTTAGAAAAACTGA

Downstream 100 bases:

>100_bases
CGTCGCGCGGCGGCGCCGCGTCGATCCGCCAACATGCGGCGCGGGCCATCGGCCCGCCATCGATAACGACGAACCGAACG
AGAGAGACCATGCGTGCCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFRQACPDVDLQVLTSHALADFARDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPGLVARLGMPATPAELVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAAQADGAASMTGAA

Sequences:

>Translated_305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFRQACPDVDLQVLTSHALADFARDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPGLVARLGMPATPAELVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAAQADGAASMTGAA
>Mature_305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFRQACPDVDLQVLTSHALADFARDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPGLVARLGMPATPAELVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAAQADGAASMTGAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=41.296928327645, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=33.9100346020761, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.8474576271186, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=26.8292682926829, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786401, Length=267, Percent_Identity=28.0898876404494, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787128, Length=272, Percent_Identity=25, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081978, Length=266, Percent_Identity=31.203007518797, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=26.4, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=24.7311827956989, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=210, Percent_Identity=31.9047619047619, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=192, Percent_Identity=24.4791666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32763; Mature: 32763

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFRQA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
CPDVDLQVLTSHALADFARDGVDVAIRHGLGRYIGLCSERLLTVEIVALASPGLVARLGM
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHEECCC
PATPAELVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
CCCHHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCH
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAAQADGAAS
HHHHHHHHCCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
MTGAA
CCCCC
>Mature Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFRQA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
CPDVDLQVLTSHALADFARDGVDVAIRHGLGRYIGLCSERLLTVEIVALASPGLVARLGM
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHEECCC
PATPAELVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
CCCHHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCH
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAAQADGAAS
HHHHHHHHCCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
MTGAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]